INPhINIT Fellowship @ ITQB NOVA
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Cyber Suber: Modeling and Enhancing the Antimicrobial Properties of Suberin (Manuel N. Melo)
CENTRE
ITQB - Microbiologia Molecular, Estrutural e Celular (MOSTMICRO)
AREA OF KNOWLEDGE
Life Sciences Panel
GROUP OF DISCIPLINES
Biotechnology, Bioinformatics, Pharmaceuticals, Food Technology
GROUP LEADER
Dr. Manuel Nuno de Sousa Pereira Simões de Melo (supervisor); Cristina Maria da Costa Silva Pereira (co-supervisor)
m.n.melo@itqb.unl.pt
spereira@itqb.unl.pt
RESEARCH PROJECT/RESEARCH GROUP
Multiscale Modeling Lab website
https://www.itqb.unl.pt/labs/multiscale-modeling
POSITION DESCRIPTION
-Research Project / Research Group Description:
Suberin, the main component of cork, is a ubiquitous plant polymer that builds a defensive barrier in the cell wall of plants. The Applied and Environmental Mycology (AEM) lab at ITQB NOVA developed an ionic liquid-based method that allows the extraction of suberin while retaining its native barrier properties, including antimicrobial. In this multidisciplinary project we will use biomolecular coarse-grain modeling, to characterize the macromolecular structure, post-extraction self-assembly, solvent interactions, and antimicrobial activity of suberin structural variants. Computational work will be carried out at the Multiscale Modeling Lab, under Manuel N. Melo’s supervision. It will be experimentally validated at the AEM lab, under Cristina Silva Pereira’s supervision, employing nuclear magnetic resonance (NMR), gas chromatography tandem mass spectrometry (GC-MS), and advanced microscopy methods to monitor suberin’s physicochemical properties and bactericidal activity. From this combined effort, strategies will be sought to then potentiate the antimicrobial properties of suberin, by adjusting its extraction process, chemical treatment, or final formulation.
By aiming to develop new antimicrobial solutions, this project addresses the critical ad timely problem of bacterial resistance. The use of suberin further connects the outcomes of this project as added value to the cork industry, of which Portugal and Spain, the hosts of INPhINIT, are the world’s two top producers and exporters. To enlarge the diversity of functional structural variants of suberin other raw materials will be explored, e.g. potato peel, Pinus barks and roots, creating alternative value chains.
-Job position description:
The candidate to this position should have a background in the general areas of Biochemistry, Chemistry, or Biology. They should be motivated to work in a multidisciplinary project that combines computation with experiment, under the supervision and co-supervision of Manuel N. Melo and Cristina Silva Pereira, two top researchers in their fields.
The candidate will receive specific training in advanced coarse-grain modeling methods — which is an area of active growth — and in NMR, CG-MS and microscopy. The candidate will also be integrated in the MolBioS PhD Program, which will complement the training with its comprehensive curricular component on microbiology and structural biology. Beyond the areas of specific training, it is expected that after this PhD project the candidate will develop valuable expertise in computer programming and in the use of LINUX operating systems.
On a day-to-day basis, the candidate will report to the supervisor and co-supervisor, and develop work to tackle the following objectives:
- Write software to build suberin connectivity models in accordance with experimental input and for different extraction conditions;
- Parameterize and simulate coarse-grain molecular models for the suberin polymer and for the membranes of model organisms;
- Describe and enhance the antimicrobial properties of suberin;
- Enhance shelf life of suberin-based products;
- Experimentally validate hypotheses stemming from computational work.
OTHER RELEVANT WEBSITES
Applied and Environmental Mycology Lab website
https://itqbnovaaem.wixsite.com/aem-lab
Exploring anammox bacteria towards a more sustainable environment (Filipe Folgosa)
CENTRE
ITQB - Microbiologia Molecular, Estrutural e Celular (MOSTMICRO)
AREA OF KNOWLEDGE
Life Sciences Panel
GROUP OF DISCIPLINES
Human Biology, Microbiology, Molecular Biology, Genetics, Cell Biology, Genomics and Proteomics, Biochemistry, Basic Neuroscience
GROUP LEADER
Dr. Filipe dos Santos Folgosa
f.folgosa@itqb.unl.pt
RESEARCH PROJECT/RESEARCH GROUP
“Functional Biochemistry of Metalloenzymes”’s laboratory.
https://www.itqb.unl.pt/labs/Functional-Biochemistry-of-Metalloenzymes
POSITION DESCRIPTION
-Research Project / Research Group Description:
The growing environmental awareness has imposed new global challenges in order to find more efficient solutions to wastes’ management, inevitably increasing its associated costs. Wastewater (WW) management is one of the current issues, presenting several technological limitations that impairs its utilization in an efficient and global manner. The use of microorganisms as a supplement in WW’s treatment facilities to improve efficiency at lower costs is a solution.
Anammox bacteria have shown good performance in WW treatment as they are able to bypass the denitrification step of the nitrogen cycle, do not require expensive carbon sources and are able to survive to temperatures up to 85oC. However, a better understanding of the anammox process is needed to tackle some of its limitations.
This proposal aims to clarify the anammox cycle by uncovering its NO generation tools as well as the ability of these organisms to utilize different extracellular substrates to accomplish this process. To tackle this objective, we intend to understand 1) the role of two proteins, identified as participants of the NO generation; 2) to identify other proteins involved in this mechanism and 3) how they influence the efficiency of these organisms in WW treatment.
Understanding these aspects of the anammox process will have a great impact on the use of these bacteria for biotechnological applications, contributing for more efficient wastewater treatment methodologies, namely for ammonium bioremediation. This will contribute to mitigation of one of the most alarming environmental problems in the World highlighted in the Goal 6 of the 2030 UN Agenda for Sustainable Development.
The work proposed will be performed in the “Functional Biochemistry of Metalloenzymes” laboratory, which has a solid experience and is internationally recognized in this research field. This will give to the student the access to all the equipment and know-how necessary for the successful accomplishment of the project.
-Job position description:
The successful candidate selected for the INPhINIT Fellowships Programme will engage in the “Molecular Biosciences” PhD programme coordinated by ITQB NOVA. Our laboratory is part of the MOSTMICRO-ITQB Research Unit, also coordinated by ITQB-NOVA, a renowned academic and research centre in Portugal. ITQB NOVA carries out research and postgraduate training in life sciences, chemistry and associated technologies, aimed at improving human health and the environment. The unique conditions at ITQB NOVA provide an excellent environment for young students to develop a research career.
The successful candidate will have the opportunity to learn several techniques such as cloning, expression and protein purification, biochemical and spectroscopic, structural and functional characterization of proteins. Finally, at the last stage, the candidate will also learn to produce complemented bacterial strains that will be used in laboratory scale WW treatment studies.
By participating on this project, the successful candidate will also have the chance to engage in international stays in other renowned research centers. A thorough formation in different skills (such as scientific writing, oral communication) will also be offered by the host institution.
It is expected that the accomplishment of the goals established for this proposal will translate into high quality publications and/or patent submissions. In a later stage, it is also expected that the work performed will translate into the creation of a new alternative to the current WW treatment processes. Also, due to the nature of this research work, it is also expected that it becomes attractive to science dissemination activities not only in international scientific meetings but also to the society in general using a diverse set of activities and approaches, for example in ITQB NOVA Open Day.
OTHER RELEVANT WEBSITES
“Molecular Biosciences” PhD programme
https://www.itqb.unl.pt/education/phd-molecular-bioscience/phd-molecular-bioscience
ITQB NOVA
Exploring the dynamics of the interaction between mammalian host cells and facultative intracellular bacterial pathogens (Pedro Matos Pereira)
CENTRE
ITQB - Microbiologia Molecular, Estrutural e Celular (MOSTMICRO)
AREA OF KNOWLEDGE
Life Sciences Panel
GROUP OF DISCIPLINES
Human Biology, Microbiology, Molecular Biology, Genetics, Cell Biology, Genomics and Proteomics, Biochemistry, Basic Neuroscience
GROUP LEADER
Dr. Pedro Matos Pereira
pmatos@itqb.unl.pt
RESEARCH PROJECT/RESEARCH GROUP
IMIB group at ITQB NOVA webpage
https://www.itqb.unl.pt/research/biology/intracellular-microbial-infection-biology
POSITION DESCRIPTION
-Research Project / Research Group Description:
In Europe, the burden caused by antibiotic resistant bacterial infections is equivalent to influenza, HIV/Aids and Tuberculosis combined. A chief factor suggested to contribute for this heavy socio-economic burden is the capacity of bacterial pathogens, which until recently were thought to be extracellular, to infect, persist and divide inside host cells. At IMIB we are interested in understanding the mechanisms underpinning the interaction of these facultative intracellular bacterial pathogen with host cells. With this objective in mind, we are interested in exploring the interplay between these facultative intracellular bacterial pathogens and host-cells autonomous immunity (mechanisms host cells use to detect and clear intracellular pathogens) in a context of bacterial infection and super-infection (bacterial plus viral infection). We also want to develop ways of streamlining the discovery of molecules with antimicrobial potential, while reducing the need for the use of animal models. As such we want to build organ-on-a-chip platforms with robust readouts, compatible with different technologies (from imaging to genomics).
-Job position description:
The selected applicant will undertake an exciting and interdisciplinary PhD project involving molecular biology, microbiology, mammalian cell biology and complex multi-cell systems (e.g. organ-on-a-chip), advanced fluorescence microscopy (e.g. super-resolution microscopy) and image analysis.
OTHER RELEVANT WEBSITES
GoogleSchoolar profile of team leader Pedro M. Pereira
https://scholar.google.com/citations?user=I-dQvQ0AAAAJ&hl=en
Linkedin profile of team leader Pedro M. Pereira
https://www.linkedin.com/in/pedro-matos-pereira-53494576/
Protein disorder-based mechanisms of host subversion by bacterial pathogens (Tiago N. Cordeiro)
CENTRE
ITQB - Microbiologia Molecular, Estrutural e Celular (MOSTMICRO)
AREA OF KNOWLEDGE
Life Sciences Panel
GROUP OF DISCIPLINES
Human Biology, Microbiology, Molecular Biology, Genetics, Cell Biology, Genomics and Proteomics, Biochemistry, Basic Neuroscience
GROUP LEADER
Dr. Tiago Neto Cordeiro
tiago.cordeiro@itqb.unl.pt
RESEARCH PROJECT/RESEARCH GROUP
The site describes the research interests and ongoing research projects in the Dynamic Structural Biology lab @ ITQB, headed by Tiago N Cordeiro. The site lists the present members of the group and also includes links to recent publications.
https://www.itqb.unl.pt/research/biological-chemistry/dynamic-structural-biology
POSITION DESCRIPTION
-Research Project / Research Group Description:
Emerging infections are a worldwide burden to human public health, reflecting the remarkable capability of pathogens to subvert host processes and immunity, often by producing host-like proteins as a way to control host signaling during infection.
Although intrinsically disordered proteins (IDPs) represent only 5% of typical bacterial proteomes, it has become clear that they control critical aspects of bacterial biology. We recently found that bacterial pathogens can produce disordered effector proteins that display a range of host-like interactions to modulate human signaling and assist infection. Given the importance of IDPs in eukaryotic signaling and regulation, such disordered effectors emerge as an efficient way to take over host processes. Our lab aims to illuminate how these sophisticated pathogen-encoded proteins work inside host cells from an integrative structural biophysics perspective. We focus on pathogen-host interactions mediated by type III secretion system (T3SS) effectors produced by life-threatening human pathogens. Within this framework, the future candidate will investigate the role of phase-separation in virulence by characterizing liquid-like phase-separated states forms of pathogen-host protein complexes.
She/He will combine various biophysical methods relevant to state-of-the-art integrated structural biology and single-molecule fluorescence microscopy exploiting directly from preliminary results and a proven track of the lab's expertise. Elucidating the dynamic-structural principles underlying the function of disordered effectors that mimic host-like IDPs to drive liquid-liquid phase-separation will provide essential insights into the mechanisms of host subversion and potentially establish a basis for anti-infection strategies.
Key words: Intrinsically disordered proteins, Host-Pathogens Interactions, Integrative structural biology and Single-molecule biophysics.
-Job position description:
The candidate will be responsible for determining the structure, dynamics, and molecular interactions of biological assemblies mediated by disordered proteins encoded by pathogensto control host cells. In particular, the project will focus on liquid-liquid separated forms of virulent effectors bound to host components associated with blocking host immune response during infection.
The project will be performed in the context of a broader collaboration between several groups at the ITQB-NOVA, aiming to decipher the structural bases of pathogen-driven phase separation and in line with a recently funded research project. Besides our lab, the candidate will work closely with Zach Hensel's and Carlos Frazão's Labs. As such, mentorship and knowledge transfer will occur through distinct channels, expertise, and point-of-views.
The project is a primary focus of the lab, with facilities for molecular biology, protein handling and expression, and access to state-of-the-art technology platforms dedicated to structural biology and bioimaging.
We expect:
The ideal candidate will be exposed to a broad range of research questions that require a genuine interest in basic biochemistry to advanced structural biology and single-molecule imaging methods. Also, with a curiosity-driven mindset to collaborate well within a multidisciplinary environment and a positive attitude towards challenges. MSc within a Life science discipline, such as biophysics, biochemistry, chemistry, computational biology, biology or similar will be highly appreciated.
OTHER RELEVANT WEBSITES
Research group tweets and views
https://twitter.com/CordeiroLab
The group leader research and bibliographic output
https://www.researchgate.net/profile/Tiago-Cordeiro-4
The site describes the research interests and ongoing research projects in the Single Molecule Microbiology lab @ ITQB NOVA, headed by Zach Hensel
Study of the traits that define the inheritance of the microbiome in Solanaceae and their effects: from targeted biocontrol to transgenerational priming to improve production (Juan Ignacio Vílchez)
CENTRE
GREEN-IT - Biorecursos para a Sustentabilidade
AREA OF KNOWLEDGE
Life Sciences Panel
GROUP OF DISCIPLINES
Agriculture, Veterinary Studies, Animal Production, Forest Sciences
GROUP LEADER
Dr. Juan Ignacio Vílchez
nacho.vilchez@itqb.unl.pt
RESEARCH PROJECT/RESEARCH GROUP
Online site that includes all information about group members, objectives, former projects and most relevant publications
https://www.itqb.unl.pt/research/plant-sciences/Plant-Microbiome-Interaction/iplantmicro
POSITION DESCRIPTION
-Research Project / Research Group Description:
In the last decade we have observed an increasing in climatic and productive challenges for agriculture. Facing a growing global population, these conditionings will suppose a serious problem. In this way, it is time to look for more sustainable models for agricultural management that will ensure its production and general supply in the future.
Solanaceae are among the most common crop plants. The food production together with industrial use (textile, pharmaceutical or horticulture), make this family of plants very important. However, this family has a high range of pathogens that condition their develop and production. Thus, they are capable of infecting reproductive organs, making plants susceptible to transferring them to the next generation (seedborne). This phenomenon is also reported for beneficial strains, suggesting some filtering system. We hypothesize these traits are mostly linked to Solanaceae characteristic chemical compounds (alkaloids, terpenes). Moreover, the presence of microbiota in seeds seems to depend on a series of skills as mobility, scavenging of reactive oxygen species, detoxifying or buffering compounds, cellulolytic capacity, chemotactic sensitivity, resistance stage (cystic, sporulation), or molecular camouflage.
Unrevealing these microbiota-plant traits in seedborne population would represent a revolution in terms of agricultural management: we propose to use these traits through to actively select a beneficial microbiota in next generation (seedborne), which will allow the application of biocontrol models, facing stress conditions or prepare a preventive hardening. This pathway would be essential in the fight against the effects of climate change, as well as against the soil degradation that condition the production of food and products of industrial interest, aligning our bioengineering project with the UN 2030 objectives providing a sustainable alternative and guaranteeing food production worldwide.
-Job position description:
The PhD student will participate in 4 main tasks:
- Task 1: Selection of species from Solanaceae family, their propagation and adjustment of conditions to carry out comparative studies.
- Task 2: Greenhouse tests to determine the relationship with the microbiota: isolation of strains, molecular pathways and phenotyping.
- Task 3: Analysis of the effects of the selected compounds on the microorganisms.
- Task 4: Testing of compounds to influence the selection of strains within the natural microbiota in the next generation (seedborne).
Among others, this student will be involved in the use of devices and software:
- HPLC and GC-MS.
- Multiskan™ FC Microplate Photometer.
- Scanning Electron Microscope, bright field and confocal fluorescence microscopes.
- Phenotyping devices as MultispeQ, FluorPen FP 110 or ThermaCAM7.
- Cytoscape v3.0.
- EZRhizo and RhizoV (root architecture analysis).
At the end of the project, we expect the student will be able to develop independently technics as:
- Culture-dependent microbiota studies
- In vitro/in planta tests
- Greenhouse and field tests
- Microscopy techniques
- Molecular biology
- Chemical treatments
- HQ-sequencing and analysis
Moreover, our research group together with all groups in our research center (ITQB) and research unit (GREEN-IT), we have an internal collaboration network. Since we have multiple common programs and projects, our students have access to specific equipment, facilities, services, techniques and resources, as well as periodical training and assistance in their use. They will be as well guided in paper writing and conferences presentations. Finally, iPlantMicro Lab has an extensive network of collaborators, including high-impact multidisciplinary groups in Spain, Ireland, France, UK, Germany, Turkey, Brazil, Argentina, India, Korea and China. The student would have access to international training, to specialized research technologies, and to the advice of some of the highest-level experts in the world.
OTHER RELEVANT WEBSITES
Website of iPlantMicro Lab at GREEN-IT Unit
https://www.itqb.unl.pt/green-it/groups/iplantmicro
Research Gate of PI
https://www.researchgate.net/profile/Juan-Ignacio-Vilchez
PI’s ORCID
https://orcid.org/0000-0003-4524-7384
The Life electric: structural and functional characterization of cytochromes that enable bacteria to survive by producing electricity (Ricardo O. Louro)
CENTRE
ITQB - Microbiologia Molecular, Estrutural e Celular (MOSTMICRO)
AREA OF KNOWLEDGE
Life Sciences Panel
GROUP OF DISCIPLINES
Human Biology, Microbiology, Molecular Biology, Genetics, Cell Biology, Genomics and Proteomics, Biochemistry, Basic Neuroscience
GROUP LEADER
Dr. Ricardo O. Louro
louro@itqb.unl.pt
RESEARCH PROJECT/RESEARCH GROUP
Inorganic Biochemistry and NMR lab website
https://www.itqb.unl.pt/labs/inorganic-biochemistry-and-nmr/home
POSITION DESCRIPTION
-Research Project / Research Group Description:
In line with the notion expressed by the Nobel Prize winner Albert Szent-Györgyi that ‘Life is nothing but an electron looking for a place to rest’, some bacteria are capable of living by delivering electrons to electrical circuits. This enabled the development of BioElectrical Technologies that are powered by the microbial metabolism, and that operate in conditions of low ecological footprint. Despite great strides in the development of these technologies, no detailed characterization of the redox enzymes at the beginning of these novel bioenergetics redox chains has been reported thus far. This is the crucial biochemical step where the metabolism diverges from the traditional aerobic respiration with oxygen or anaerobic respiration of numerous compounds such as sulfate or nitrate that have in common the fact of being soluble. These key enzymes interface the quinone pool of the cytoplasmic membrane with the specific electron transfer chains that deliver the electrons to the electrical circuit outside of the cell. In the model electricity generating bacteria of the genus Shewanella, the key enzyme is CymA. It is a tetraheme cytochrome of ~20kDa with a N-terminal alpha helix inserted in the cytoplasmic membrane. NMR spectroscopy is uniquely suited for the detailed structural and functional characterization of an enzyme of this size that is membrane bound. Its characterization will conclude the structural and functional characterization of this unique electron transfer chain allowing, for the first time, to have a complete functional and structural picture of how “Life Electric” operates
The laboratory is composed by a mature research team headed by the PI and another assistant researcher that are recognized leaders in the international panorama of biochemical studies in BioElectrochemical Technologies. We are currently funded by one national research project, two European projects, one bilateral project and engaged in a COST action, all relevant for this proposal.
-Job position description:
This PhD research work comprises
i)Protein expression and purification. The candidate will follow-up on methodologies developed in the lab for the expression of the target proteins and develop strategies for their efficient expression and purification from cells grown in minimal medium. This will enable the preparation of isotopically labelled protein for NMR spectroscopy studies.
ii)NMR data collection. The candidate will prepare samples with protein in the oxidized and in the reduced state. Cyanide will be used as strong-field ligand to convert the high-spin heme into its low spin form to facilitate spectral assignment. Standard strategies methods will be used for data collection of the diamagnetic reduced samples, whereas non-standard methods recently developed by the host lab will be used for data collection in the oxidized sample.
iii)Signal assignment and characterization of interactions with physiological partners. Computer-aided signal assignment will be performed iteratively with additional data collection that may prove to be necessary to complete the task. The assignment will provide the opportunity to identify the binding region of physiological redox partners.
iv)Structural calculation. Nuclear overhauser effect distance constrains will be used for structural calculation of the reduced diamagnetic protein whereas these data will be complemented by paramagnetic constrains (pseudocontact shifts and paramagnetic relaxation enhancements) will be used to calculate the structure in the oxidized paramagnetic state. T1, T2 and heteroniclear NOE will be used to assess the amplitude and timescale of the motions of the protein, and how these change with redox state and upon binding with physiological partners.
This workplan provides advanced training on the whole pipeline of modern structural biology workflow using the top-rated know-how and facilities of the host lab and privileged access to European research infrastructure.
The role of the ER membrane complex (EMC) in the biogenesis of the transmembrane proteins (Pedro Domingos)
CENTRE
ITQB - Microbiologia Molecular, Estrutural e Celular (MOSTMICRO)
AREA OF KNOWLEDGE
Life Sciences Panel
GROUP OF DISCIPLINES
Human Biology, Microbiology, Molecular Biology, Genetics, Cell Biology, Genomics and Proteomics, Biochemistry, Basic Neuroscience
GROUP LEADER
Dr. Pedro Manuel Dias Neto Domingos
domingp@itqb.unl.pt
RESEARCH PROJECT/RESEARCH GROUP
Website of the Laboratory of Cell Signalling in Drosophila
https://www.itqb.unl.pt/research/biology/cell-signaling-in-drosophila
POSITION DESCRIPTION
-Research Project / Research Group Description:
The Laboratory of Cell Signalling in Drosophila, headed by Pedro Domingos, has been interested in the the mechanisms regulating proteostasis in the Endoplasmic Reticulum (ER), including the physiological role of the ER stress signalling pathways during development and in disease models [1,2]. Recently, we were awarded by La Caixa Foundation a collaborative grant to study the organismal role of the ER Membrane Complex, using both Drosophila (in Pedro Domingos’ group) and mammallian models (in the Laboratory of Membrane Traffic, headed by Colin Adrain, the project leader of La Caixa Foundation grant HR17-00595 “Organismal role of the ER membrane complex: a conserved machinery for membrane protein biogenesis”).
The biogenesis of membrane proteins in the ER often involves the import machinery comprised by the signal recognition particle (SRP) and the Sec61 import channel. SRP recognizes hydrophobic regions present in N-terminal signal peptides or transmembrane domains (TMDs) within client polypeptides emerging from the ribosome, coordinating their Sec61-dependent co-translational insertion into the ER membrane [3].
Another family of membrane proteins - the tail anchored (TA) proteins - contain a TMD at their extreme C-terminus, which is shielded within the ribosome during translation. These proteins undergo post-translational membrane insertion, mediated by a complex called the TRC (TMD recognition complex) in mammals, also called the GET (guided entry of TA) pathway in yeast [3].
Recently, it was shown that a third protein complex called the EMC (ER membrane complex) [4] is required for the import into the ER of a subset of multi-TMD and TA proteins that contain TMDs with low hydrophobicity [5] [6] [7].
-Job position description:
The PhD student position will exploite the observation that EMC TA client proteins exhibit reduced TMD hydrophobicity [5]. We did a pilot experiment to predict EMC clients in the Drosophila genome. Our analysis identified ~300 TA proteins from which we isolated a group of proteins of reduced TMD hydrophobicity, which we screened for candidate EMC clients. This analysis revealed 2 potential novel EMC TA clients: Fan, which controls sperm individualization [8], and Xport-A [9]. Intriguingly, Xport-A is required for the biogenesis of both Rhodopsin-1 (Rh1) and TRP [9], proteins whose biogenesis is affected in EMC mutants [10], raising the possibility that EMC may affect the biogenesis of Rh-1 and TRP indirectly, by controlling the biogenesis of Xport-A. The PhD candidate will test this hypothesis in a set of rescue experiments, where constructs of Xport-A with an increasingly more hydrophobic TMD, will be expressed to attempt to rescue Xport-A and Rh-1 biogenesis. The PhD candidate will expand our screen for EMC clients in Drosophila, in the context of the consortium that was recently funded by La Caixa Foundation.
REFERENCES
1. Ryoo, H.D., et al. Embo J, 2007. 26(1): p. 242-52.
2. Coelho, DS, et al. Cell Reports, 2013. 5(3):791-801.
3. Shao, S. and R.S. Hegde. Annu Rev Cell Dev Biol, 2011. 27: p. 25-56.
4. Christianson, J.C., et al. Nat Cell Biol, 2011. 14(1): p. 93-105.
5. Guna, A., et al. Science, 2018. 359(6374): p. 470-473.
6. Shurtleff, M.J., et al. Elife, 2018. 7.
7. Chitwood, P.J., et al. Cell, 2018. 175(6): p. 1507-1519 e16.
8. Ma, Z., Z. Liu, and X. Development, 2010. 137(22): p. 3775-84.
9. Rosenbaum, et al. Neuron, 2011. 72(4): p. 602-15.
10.Satoh, T., et al. Elife, 2015. 4:e06306
OTHER RELEVANT WEBSITES
Website of ITQB-UNL, host of the Laboratory of Cell Signalling in Drosophila
Website of the Laboratory of Membrane traffic, headed by Colin Adrain, a collaborator in this project.
http://www.igc.gulbenkian.pt/cadrain
