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Methods for Biosciences II (Tools for Structural Biology)
Coordinators
Carlos Frazão / Ricardo Louro
Objectives
This course aims at offering PhD students a sound and proficient understanding of the main methodologies that support Structural Biology, in a thematic and complementary perspective, towards the analysis and understanding of structure-function relationships in biological systems.
This is a structuring curricular unit within the profile “Molecular Mechanisms of Biological Processes”.
Syllabus
Methods for 3D structural determination of biological macromolecules:
a. X-ray Crystallography.
b SAXS.
c. NMR (2D and 3D).
d. Electron Microscopy (cryo-EM)
e. EPR.
f. Comparison of structural determination methods.
Evaluation
Evaluation of the acquisition by the students of concepts and techniques taught and discussed, will be accomplished throughout the different practicum sessions, when dealing with applications of the techniques under study or solving questions or tasks in practical cases presented during the various sessions.
Main Bibliography
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“Practical Approaches to Biological Inorganic Chemistry” 2nd Edition, edited by Ricardo O. Louro and Robert R. Crichton, (2019) Elsevier B.V. Amsterdam, The Netherlands ISBN 978-0-444-64225-7
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“Biomolecular Crystallography: Principles, Practice, and Application to Structural Biology”, B. Rupp, Garland Science (2009).
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“Structural Biology; Practical NMR applications” Q. Teng, Springer Science +Business Media, Inc, NY (2005).
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Proteins: From Analytics to Structural Genomics (Hardcover) by Robert A. Meyers (Editor) , Vols. I and II, Wiley-VCH Verlag (2007).
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