1st CERMAX practical course on basic NMR

Scope:

A two day course with theoretical and practical sessions covering basic aspects of the technique and oriented towards both beginners and current users of NMR.

Topics:

Basic theoretical aspects of NMR. Introduction to the most commonly used 1D and 2D homonuclear and heteronuclear techniques. Data acquisition and processing with a “hands-on” approach.

Applications:

The course is open and free of charge; the practical sessions are restricted to twelve ITQB students. To apply to this course please send an e-mail to lamosa@itqb.unl.pt stating your interest accompanied by a short CV.

Tutors:

Patrick Groves, Pedro Lamosa, Helena Matias and Manolis Matzapetakis.

Program

2nd of June

  9.00 – Introduction to NMR spectroscopy  (the phenomenon, basic properties of the signal)
10.00 – Instrumental aspects of the spectrometer  

10.30 – Break

11.00 – Practical aspects of acquisition and processing  (shimming, tuning, acquisition parameters, solvent suppression, pulse programs, 90 degree pulse and T1 determination)

13.00 – Lunch Break

14.30 – Practical sessions

                                         - Sample preparation (30 min)  
                                         - Acquisition (1H, presat, 13C, APT) 
                                         - Processing and analyzing 1D data 

3rd of June

  9.00 – Introduction to 2D NMR spectroscopy  (COSY and TOCSY)
10.00 – NOE and NOESY   

10.30 – Break

11.00 – INEPT, HSQC and HMBC  
11.30 – Assignment strategies and small molecule structure (a tutorial)

13.00 – Lunch Break

14.30 – Practical sessions
                                           - Acquisition (COSY, HSQC)  
                                           - Processing and analyzing 2D data