5th CERMAX practical course on basic NMR

5th CERMAX practical course on basic NMR

The course is open and free of charge for NMR network members but practical sessions are restricted to 12 students. To apply please send an e-mail to lamosa@itqb.unl.pt stating your interest accompanied by a short CV.

Program

2nd of July
9.00 – Introduction to NMR spectroscopy (the phenomenon, basic properties of the signal).
10.00 – Instrumental aspects of the spectrometer /Rules for spectrometer use.
10.45 – Break
11.15 – Introduction to TopSpin software.
13.00 – Lunch break
14.30 – Practical aspects of acquisition and processing (acquisition parameters, pulse programs, 90º pulse and T1 determination).
15.30 – Break
16.00 – Spectrometer Guided tour and sample preparation

3rd of July
10.00 – The Nuclear Overhauser Effect
10.30 – Break
11.00 – Practical session I
Acquisition (1D, presat, p90, APT)
Processing and analyzing 1D
13.00 – Lunch break
14.30 – Practical sessions I (cont)

4th of July
9.00 – Introduction to 2D NMR spectroscopy (Homonuclear correlation)
10.00 – INEPT, HSQC and HMBC
11.00 – Break
11.30 – DOSY
12.15 – Protein structure
13.00 – Lunch break
14.30 – Practical session II
Acquisition (COSY, HSQC)
Processing and analyzing 2D

5th of July
10.00 – Assignment strategies in small molecules (tutorial and exercises)
11.00 – Break
11.30 – Assignment strategies (cont)
13.00 – Lunch break
14.30 – Practical session III
Acquisition exercises

Faculty:

• Helena Matias
• Manolis Matzapetakis
• Patrick Groves
• Pedro Lamosa