RNEM Course on Protein Identification by Mass Spectrometry
| When |
27 Jan, 2010 09:00 AM
to
28 Jan, 2010 06:00 PM |
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The first edition of this Training Course to be held at ITQB-UNL is dedicated to researchers using or willing to use mass spectrometry methods for protein identification. Participants will have the opportunity to meet the ITQB/IBET-Analytical Services Unit facilities and profit from RNEM Institutions expertise.
RNEM Institutions involved: ITQB-UNL FC-UL CNC-UC UA
Main objectives
- to give practical insight into the most used MS strategies for protein identification
- to provide hands-on experience on crucial areas, sample preparation and data evaluation
- to present major methods advantages and limitations
- to supply information on the preparation of protein identification data for publication
Training Course
duration: 2 days
dates: 27th-28th January 2010
Maximum number participants: 40, first 20 registration will allow participation in supervised training
Registration fee (includes course documentation, coffee breaks and lunches):
Normal: 50 euros
Students (undergraduate, master and PhD): 40 euros
Training Course content
Seminars
- Protein identification and characterization using MS data: a general overview
- Fundamentals on Soft Ionization and MS Instrumentation
- LC-ESI-MS/MS and MALDI-TOF-TOF data acquisition, processing and database search
- Protein quantification by mass spectrometry approaches
- Characterization of post-translational modifications
- The high-resolution profits
Demonstration
- Protein Digestion
- LC-ESI-MS/MS and MALDI-TOF-TOF data acquisition
Supervised training
- Data processing and data base search
- De novo sequencing
Schedule
Day 1
- 9:00-10:00 Seminar: Protein identification and characterization using MS data: a general overview
- 10:00-11:00 Seminar: Fundamentals on Soft Ionization and MS Instrumentation
- Coffee break
- 11:30-12:30 Seminar: LC-ESI-MS/MS and MALDI-TOF-TOF data acquisition, processing and database search I
- Lunch
- 14:00-15:00 Seminar: LC-ESI-MS/MS and MALDI-TOF-TOF data acquisition, processing and database search II
- 15:00-17:00 Demonstrations
Day 2
- 9:00-10:00 Seminar: Protein quantification by mass spectrometry approaches
- 10:00-11:00 Seminar: Characterization of post-translational modifications
- Coffee break
- 11:30-12:30 Seminar: The high-resolution profits
- Lunch
- 14:00-17:00 Supervised training
Course Presentations
User name and password required - please contact course organizers
