RNEM Course on Protein identification by Mass Spectrometry

This Hands-On Training Course to be held at ITQB-UNL is dedicated to researchers using or willing to use mass spectrometry methods for protein identification. Participants will have the opportunity to bring their samples.

Main objectives

- to give practical insight into the most used MS strategies for protein identification
-  to provide hands-on experience on crucial areas, sample preparation and data evaluation
- to present major methods advantages and limitations
- to supply information on the preparation of protein identification data for publication

Important Information

Dates: 2nd-4th November 2011
Duration: 3 days
Registration deadline: 17 October
Payment deadline: 24 October
Maximum number participants: 30
Registration fee (includes course documentation, coffee breaks, lunches and protein identification in two samples): 200 euros

Payment information

Please include in the description of the payment your name and the words 'RNEM Course'. Also, all bank expenses should be covered by the participants.

Payment details:

Name: CTQB – Projectos NPS
Bank: Caixa Geral de Depósitos
Address: Rua Prior do Crato, 70 A - 1300 Lisboa
Bank Branch: Alcântara
NIB (for Portugal): 0035 0027 0006 4688 4300 3
IBAN (for international): PT50 0035 0027 0006 4688 4300 3
SWIFT/BIC: CGDIPTPL

Registration Form

Please e-mail this form before 17th October 2011 to palhinhas@itqb.unl.pt

Training Course content

Seminars

• Protein identification and characterization using MS data: a general overview
• Fundamentals on Soft Ionization and MS Instrumentation
• LC-ESI-MS/MS and MALDI-TOF-TOF data acquisition, processing and database search
• Processing protein identification data for publication
• Pathway analysis

Hands-On

•   Protein Digestion
•   Protein identification by MALDI-TOF-TOF

Supervised training

• Data processing and data base search
• De novo sequencing
• Preparing data for identification
• Pathway analysis

Demonstration

• LC-ESI-MS/MS data acquisition

Course Schedule

download the course schedule in PDF

Contacts

Ana Varela Coelho (Course Coordinator)  varela@itqb.unl.pt
Rui Palhinhas (Secretariat)  palhinhas@itqb.unl.pt