Corynebacterium glutamicum tailored for efficient isobutanol production
Bastian Blombach- University of Ulm, Germany
| When |
16 Mar, 2011
from
10:30 am to 11:30 am |
|---|---|
| Where | Auditorium |
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ITQB- Seminar
Title: "Corynebacterium glutamicum tailored for efficient isobutanol production"
Speaker: Bastian Blombach
Affiliation: University of Ulm, Germany
Host: Helena Santos- Head of Cell Physiology and NMR
Abstract:
We recently engineered Corynebacterium glutamicum for aerobic production of 2-ketoisovalerate by inactivation of the pyruvate dehydrogenase complex, pyruvate:quinone oxidoreductase, transaminase B, and additional overexpression of the ilvBNCD genes, encoding acetohydroxyacid synthase, acetohydroxyacid isomeroreductase, and dihydroxyacid dehydratase. Based on this strain, we engineered C. glutamicum for the production of isobutanol from glucose under oxygen deprivation conditions by inactivation of L-lactate and malate dehydrogenases, implementation of ketoacid decarboxylase from Lactococcus lactis, alcohol dehydrogenase 2 (ADH2) from Saccharomyces cerevisiae, and expression of the transhydrogenase genes pntAB from Escherichia coli. The resulting strain produced isobutanol with a substrate specific yield (YP/S) of 0.60 ± 0.02 mol per mol of glucose. Interestingly, a chromosomally encoded alcohol dehydrogenase rather than the plasmid-encoded ADH2 from S. cerevisiae was involved in isobutanol formation with C. glutamicum and overexpression of the corresponding adhA gene increased the YP/S to 0.77 ± 0.01 mol isobutanol per mol of glucose. Inactivation of the malic enzyme significantly reduced the YP/S, indicating that the metabolic cycle consisting of pyruvate and/or phosphoenolpyruvate carboxylase, malate dehydrogenase and malic enzyme is responsible for the conversion of NADH+H+ to NADPH+H+. In fed-batch fermentations with an aerobic growth phase and an oxygen-depleted production phase, the most promising strain C. glutamicum ∆aceE ∆pqo ∆ilvE ∆ldhA ∆mdh (pJC4ilvBNCD-pntAB) (pBB1kivd-adhA) produced about 175 mM isobutanol with a volumetric productivity of 4.4 mM h-1, and showed an overall YP/S of about 0.48 mol per mol of glucose in the production phase.
