Marrying Qual and Qual Aspects in - Omics World
Michaela Scigelova, Thermo Fisher Scientific, Bremen, Germany
| When |
02 Nov, 2011
from
11:00 am to 12:00 pm |
|---|---|
| Where | Auditorium |
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Seminar
included in the “Hands-On Course on Protein Identification by Mass Spectrometry“
Title: Marrying Qual and Qual Aspects in -Omics World
Speaker: Michaela Scigelova
Affiliation: Thermo Fisher Scientific, Bremen, Germany
Over the last decade we have seen considerable developments in applying LC-MS techniques within the areas of various –omics. From purely qualitative approach focused on cataloguing analytes in samples representing various origin or environmental conditions, the field has matured into a discipline relying heavily on quantitative analysis. Albeit relative quantitation methods have their place within discovery strategies, it is the absolute quantitative dimension that has become a clear focus of leading biomarker research projects.
Absolute targeted quantitation approach has been addressed by triple quadrupole-based mass spectrometers (MS). The notion of high throughput and cost efficiency historically associated with such an instrumentation, however, has been challenged recently by large biomarker discovery studies.1,2 Instead, they highlighted the importance of high resolution MS not only in the discovery phase, where they have been already widely in use, but also for a considerable part of the biomarker verification workflow, effectively engaging the triple quadrupole-based systems only at the very last step of the biomarker verification process.
The strategy of ‘directed analysis’ which relies on high resolution mass spectrometers for a substantial (and ever expanding) part of the biomarker verification workflow is merging the qual and quan aspects of the analytical work. Mass spectrometric instrumentation must address the challenges of this double-edged application. The presentation will introduce Q Exactive, a new hybrid mass spectrometer, and highlight unique capabilities resulting from a marriage between two well known mass measurement devices, a quadrupole mass filter and an Orbitrap analyzer.
References:
1. Whiteaker J. R. et al. Nat.Biotechnol. 2011, doi:10.1038/nbt.1900
2. Addona T. A. et al., Nat. Biotechnol. 2011, doi:10.1038/nbt.1899
