SCAN: Photoprotection systems in Quercus ilex L. and the application of the Near-Infrared Reflectance Spectroscopy (NIRS) as an ecophysiological tool to detect oxidative stress
Marta Pintó-Marijuan Post-Doctoral
| When |
15 Jun, 2011
from
12:00 pm to 01:00 pm |
|---|---|
| Where | Auditorium |
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iCal
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ITQB-SCAN Seminar
Title: Photoprotection systems in Quercus ilex L. and the application of the Near-Infrared Reflectance Spectroscopy (NIRS) as an ecophysiological tool to detect oxidative stress
Speaker: Marta Pintó-Marijuan
From: Plant Molecular Ecophysiology Laboratory
Abstract:
Quantification of chloroplast pigments and antioxidant compounds in ecophysiological studies can be a laborious and expensive task. For rapid evaluation of such compounds, we built a database correlating Near Infrared Reflectance Spectroscopy (NIRS) and chemical analyses of holm oak (Quercus ilex) leaves sampled at different years, locations and under different physiological conditions. Predictive calibration equations for the concentration of chloroplast pigments and antioxidant compounds were established. Different lyophilisation conditions (duration and addition of several preservatives) were tested to compare preservation of the lyophilized and the frozen material. Numerous results can be generated by a single measurement of about one minute. The measurement requires only a small amount of material (ca. 2 g DW), little time for material preparation (lyophilisation and grinding) and does not consume reagents.
The NIRS database was used to characterize the antioxidant compounds responses in two different studies (1) in adult holm oak leaves and resprouts after clear cut in their natural environment and (2) in the responses of holm oak resprouts under elevated CO2 in controlled-environmental conditions.
Our results showed the applicability of NIRS to plant ecological and physiological research allowing quantification of the antioxidative and photoprotective defence compounds in lyophilised Q. ilex leaves. This becomes the first NIRS database to be assembled for the evaluation of multiple pigments and antioxidants, and it provides a suitable alternative to conventional analysis for large sets of samples.
