SCAN:Biochemical Systems Analysis of the trehalose cycle in Saccharomyces cerevisiae
Luís L. Fonseca Post-Doctoral fellow at Cell Physiology and NMR Laboratory
| When |
29 Jun, 2011
from
12:00 pm to 01:00 pm |
|---|---|
| Where | Auditorium |
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ITQB- Scan Seminar
Title: Biochemical Systems Analysis of the trehalose cycle in Saccharomyces cerevisiae
Speaker: Luís L. Fonseca
From: Post-Doctoral at Cell Physiology and NMR Laboratory
Abstract:
Saccharomyces cerevisiae is a well-known model organism to study heat shock and adaptation. One of the most striking features of this organism is the accumulation of huge amounts of trehalose in response to heat stress. Trehalose is a non-reducing disaccharide of glucose, which has been extensively implicated in protection of proteins, membranes and other biomaterials against a variety of stresses. In this work we studied heat adaptation by comparing optimally grown cells (30ºC, control cells) and heat-adapted cells (by exposure to 39ºC for 40 min during growth), and used in vivo 13C NMR spectroscopy to dynamically probe the metabolism of these cultures at optimal or supra-optimal temperatures. Generalized mass action equations within the framework of the Biochemical Systems Theory were used and extended to model glucose metabolism and the trehalose cycle under the different experimental conditions examined. Time series data acquired by in vivo 13C-NMR were used to parameterize this kinetic model. From this computational analysis it was possible to determine the minimum set of temperature-dependent processes and obtain insights into how heat-adaptation changes the expression of the proteins involved in trehalose metabolism.
