SCAN:Spectro-Electrochmistry of Heme Proteins – Towards Biotechnological Application

SCAN Seminar ITQB

Title: Spectro-Electrochmistry of Heme Proteins – Towards Biotechnological Application

Speaker: Murat Sezer

From:  Raman Spectroscopy of Metalloproteins Laboratory

Abstract:

Oxygen tolerant hydrogenases and heme containing dye decolorising peroxidases are particularly interesting enzymes for biotechnological application. While hydrogenases may play a key role in the development of novel hydrogen-based energy conversion and storage technologies, dye decolorising peroxidases may be exploited as biocatalysts in the decomposition of ecologically harmful industrial dyes, and also in the development of versatile bio-electronic sensing devices. A major strategy in the design of bio-electronic devices is the immobilisation of redox-active enzymes onto conductive support materials that allow for electrochemical control of the enzymatic activity. In this talk I will show how electrochemical methods can be complemented by surface enhanced vibrational spectroscopy to screen structural features, catalytic efficacy and stability of immobilised enzymes, and their electronic coupling with the conductive support. In particular, two examples will be discussed: oxygen tolerant membrane bound [NiFe]-hydrogenase from Ralstonia eutropha H16 and recombinant dye decolorising peroxidase from Pseudomonas putida.