Cryo-EM Seminar @ ITQB NOVA - Sharon Wolf

IMpaCT project is happy to announce our Cryo-EM seminar with our expert partner guest, Sharon Wolf from the Weizmann Institute of Science.

Date: 26 July 2022

Time: 2pm Lisbon Time / 3pm CET

Location: @ ITQB NOVA 2.13 room & Webinar

Meeting ID: 811 2217 1665

Passcode: 168895

The Power of cryoSTEM Tomography

Sharon Wolf

Weizmann Institute of Science 

Cryo-electron microscopy has evolved to be a powerful method for solving the structures of macromolecules without the need for crystallization, and also for imaging ultrastructure in vitrified, close-to-native-state cells and tissues. However, today's modern electron microscopes are capable of other imaging modes than the wide-field transmission mode that is most associated with the field. IndeedScanning Transmission Electron Microscopy (STEM) is the standard imaging modality for high resolution studies in Materials Sciences, but is underutilized for biological specimens, and in particular for tomography.  I will introduce STEM tomography, and present the advantages this modality offers for studying cellular ultrastructure, chemical characterization and other unique benefits of the method.