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Cell and Process Engineering and Modeling

  1. Tenreiro MF, Almeida HV, Calmeiro T, Fortunato E, Ferreira L, Alves PM, Serra M. 2021. Interindividual heterogeneity affects the outcome of human cardiac tissue decellularization. Scientific Reports. 11: 20834. doi:10.1038/s41598-021-00226-5

  2. Tenreiro MF, Louro AF, Alves PM, Serra M. 2021. Next generation of heart regenerative therapies: progress and promise of cardiac tissue engineering. npj regenerative medicine. 6:30 (2021). doi: 10.1038/s41536-021-00140-4

  3. Sebastião MJ, Marcos-Silva L, Gomes-Alves P, Alves PM. 2021. Proteomic and Glyco(proteo)mic tools in the profiling of cardiac progenitors and pluripotent stem cell derived cardiomyocytes: Accelerating translation into therapy. Biotechnology Advances. doi:10.1016/j.biotechadv.2021.107755

  4. Isidro IA, Vicente P, Pais DAM, Almeida JI, Domingues M, Abecasis B, Zapata-Linares N, Rodriguez-Madoz JR, Prosper F, Aspegren A, Alves PM, Serra M. 2021. Online monitoring of hiPSC expansion and hepatic differentiation in 3D culture by dielectric spectroscopy. Biotechnology & Bioengineering. doi:10.1002/bit.27751

  5. Almeida HV, Tenreiro MF, Louro AF, Abecasis B, Santinha D, Calmeiro T, Fortunato E, Ferreira L, Alves PM, Serra M. 2021. Human Extracellular-Matrix Functionalization of 3D hiPSC-Based Cardiac Tissues Improves Cardiomyocyte Maturation. ACS Applied Bio Materials. 4(2): 1888. doi:10.1021/acsabm.0c01490

  6. Sousa BB, Sousa MFQ, Marques MC, Seixas JD, Brito JA, Matias PM, Bernardes GJL, Roldão A. 2020. A scalable insect cell-based production process of the human recombinant BMX for in-vitro covalent ligand high-throughput screening. Bioprocess and Biosystems Engineering. 43(9). doi:10.1007/s00449-020-02421-6

  7. Moleirinho MG, Silva RJS, Alves PM, Carrondo MJT, Peixoto C. 2019. Current challenges in biotherapeutic particles manufacturing. Expert Opinion on Biological Therapy. doi:10.1080/14712598.2020.1693541

  8. Sebastião MJ, Gomes-Alves P, Reis I, Sanchez B, Palacios I, Serra M, Alves PM. 2019. Bioreactor-based 3D human myocardial ischemia/reperfusion in vitro model: a novel tool to unveil key paracrine factors upon AMI. Translational Research. doi: 10.1016/j.trsl.2019.09.001

  9. Abecasis B, Gomes-Alves P, Rosa S, Gouveia PJ, Ferreira LS, Serra M, Alves PM. 2019. Unveiling the molecular crosstalk in a human induced pluripotent stem cell derived cardiac model. Biotechnol Bioeng. https://doi.org/10.1002/bit.26929

  10. Ana Teresa Serra, Margarida Serra, Ana Carina Silva, et al. 2018. Scalable culture strategies for the expansion of patient-derived cancer stem cell lines Stem Cells International. Stem Cells International https://doi.org/10.1155/2019/8347595

  11. Serra M, Cunha B, Peixoto C, Gomes-Alves P, Alves PM. 2018. Advancing Manufacture of hMSC Therapies: technological challenges in cell bioprocessing and characterization. Current Opinion in Chemical Engineering. 22: 226- 235. https://doi.org/10.1016/j.coche.2018.11.003

  12. Hu D, Linders A, Yamak A, et al. 2018. Metabolic Maturation of Human Pluripotent Stem Cell Derived Cardiomyocytes by Inhibition of HIF1α and LDHA Basic Science Research Cell Signaling/Signal Transduction Contractile Function Metabolism Stem Cells.1066-1079. doi:10.1161/CIRCRESAHA.118.313249.

  13. Sebastião MJ, Menta R, Serra M, et al. 2018. Human cardiac stem cells inhibit lymphocyte proliferation through paracrine mechanisms that correlate with indoleamine 2,3-dioxygenase induction and activity. Stem Cell Res Ther. 2(290):1-7. https://doi.org/10.1186/s13287-018-1010-2

  14. Samaras JJ, Abecasis B, Serra M, Ducci A, Micheletti M. 2018. Impact of Hydrodynamics on iPSC-derived Cardiomyocyte Differentiation Processes. J Biotechnol. 287(March):18-27. doi:10.1016/j.jbiotec.2018.07.028.

  15. Silva MM, Gomes-Alves P, Rosa S, et al. 2018. Full-length human CCBE1 production and purification: leveraging bioprocess development for high quality glycosylation attributes and functionality. J Biotechnol. 285(May):6-14. https://doi.org/10.1016/j.jbiotec.2018.08.015

  16. Correia C, Koshkin A, Duarte P, et al. 2018. 3D aggregate culture improves metabolic maturation of human pluripotent stem cell derived cardiomyocytes. Biotechnol Bioeng. 115(3):630-644. https://doi.org/10.1002/bit.26504.

  17. Sa JV, Kleiderman S, Brito C, Sonnewald U, Leist M, Teixeira AP, Alves PM.  2017.  Quantification of Metabolic Rearrangements During Neural Stem Cells Differentiation into Astrocytes by Metabolic Flux Analysis.  Neurochemical Research. 42, 244-253. http://dx.doi.org/10.1007/s11064-016-1907-z

  18. Teixeira AP and Fussenegger M. 2017. Synthetic biology-inspired therapies for metabolic diseases. Current Opinion in Biotechnology. 47:59–66.  http://dx.doi.org/10.1016/j.copbio.2017.06.004

  19. Carinhas N, Koshkin A, Pais DAM, Alves PM and Teixeira AP. 2017. 13C-metabolic flux analysis of human adenovirus infection: Implications for viral vector production. Biotechnol. Bioeng., 114: 195–207. https://doi.org/10.1016/10.1002/bit.26063

  20. Abecasis B, Aguiar T, Arnault E, Costa R, Gomes-Alves P, Aspegren A, Serra M, Alves PM. 2017.  Expansion of 3D human induced pluripotent stem cell aggregates in bioreactors: Bioprocess intensification and scaling-up approaches.  J Biotechnol. 246:81-93. http://dx.doi.org/10.1016/j.jbiotec.2017.01.004

  21. Isidro IA, Portela RM, Clemente JJ, Cunha AE, Oliveira R.  2016.  Hybrid metabolic flux analysis and recombinant protein prediction in Pichia pastoris X-33 cultures expressing a single-chain antibody fragment.  Bioprocess Biosyst Eng.  39(9):1351-63. https://doi.org/10.1007/s00449-016-1611-z

  22. Carinhas N, Pais DAM, Koshkin A, Fernandes P, Coroadinha A, Carrondo MJT, Alves PM, Teixeira AP. 2016. Metabolic flux profilling of MDCK cells during growth and canine adenovirus vector production. Scientific Reports 6: 23529. http://www.nature.com/articles/srep23529

  23. Sousa MFQ, Silva MM, Roldão A, Alves PM, Serra M, Giroux D, Hashimura Y, Wesselschmidt R, Lee B, Carrondo MJT. 2015. Production of oncolytic adenovirus and human mesenchymal stem cells in a single-use, Vertical-Wheel bioreactor system: Impact of bioreactor design on performance of microcarrier-based cell culture processes. Biotechnol Progress. http://dx.doi.org/10.1002/btpr.2158.

  24. Silva AC, Simão D, Sousa MFQ, Küppers C, Lucas T, Cruz P, Carrondo MJ,  Kochanek S, Alves PM 2015 Human Amniocyte-derived Cells for the Production of Adenoviral Vectors. Biotechnology Journal, http://dx.doi.org/10.1002/biot.201400765

  25. Rodrigues AF, Carrondo MJ, Alves PM, Coroadinha AS 2015. Cellular targets for improved manufacturing of virus-based biopharmaceuticals in animal cells. Trends in Biotechnology, 32(12):602-7. http://dx.doi.org/10.1016/j.tibtech.2014.09.010

  26. Duarte TM, Carinhas N, Barreiro LC, Carrondo MJT, Alves PM, Teixeira AP. 2014. Metabolic responses of CHO cells to limitation of key amino acids. Biotechnol Bioeng. Volume 111(10): 2095–2106. http://dx.doi.org/10.1002/bit.25266

  27. Rodrigues AF, Formas-Oliveira AS, Bandeira, VS, Alves PM, Hu WS, Coroadinha AS. 2013. Metabolic pathways recruited in the production of a recombinant enveloped virus: mining targets for process and cell engineering. Metab. Eng. 20: 131-137. http://dx.doi.org/10.1016/j.ymben.2013.10.001

  28. Monteiro F, Bernal V, Saelens X, Lozano AB, Bernal C , Sevilla A, Carrondo MJT, Alves PM. 2013 Metabolic profiling of insect cell lines: Unveiling cell line determinants behind system’s Productivity. Biotechnol Bioeng. 20: 131-145. http://dx.doi.org/10.1002/bit.25142

  29. Vidigal J, Dias MM, Fernandes F, Patrone M, Bispo C, Andrade C, Gardner R, Carrondo MJT, Alves PM, Teixeira AP 2013 A cell sorting protocol for selecting high-producing sub-populations of Sf9 and High FiveTM cells, J Biotech. 168 (4): 436-49.  http://dx.doi.org/10.1016/j.jbiotec.2013.10.020

  30. Carinhas N, Duarte TM, Barreiro LC, Carrondo MJT, Alves PM, Teixeira AP. 2013. Metabolic signatures of GS‑CHO cell clones associated with butyrate treatment and culture phase transition. Biotechnol Bioeng, 110 (12): 3244-57  http://dx.doi.org/10.1002/bit.24983

  31. Fernandes P, Peixoto C, Santiago VM, Kremer EJ, Coroadinha AS, Alves PM, 2013. Bioprocess development for canine adenovirus type 2 vectors. Gene Ther. Apr;20(4):353-60. http://dx.doi.org/10.1038/gt.2012.52

  32. Fernandes P, Santiago VM, Rodrigues AF, Tomás H, Kremer EJ, Alves PM, Coroadinha AS. 2013. Impact of E1 and Cre on Adenovirus Vector Amplification: Developing MDCK CAV-2-E1 and E1-Cre Transcomplementing Cell Lines. PLoS One. 2013;8(4):e60342. http://dx.doi.org/10.1371/journal.pone.0060342

  33. Fonseca CP, Fonseca LL, Montezinho LP, Alves PM, Santos H, Castro MM, Geraldes CF. 2013. (23)Na multiple quantum filtered NMR characterisation of Na(+) binding and dynamics in animal cells: a comparative study and effect of Na(+)/Li (+) competition. Eur Biophys J. Apr 6.  42(7): 503-519 http://dx.doi.org/10.1007/s00249-013-0899-8

  34. Carrondo MJ, Alves PM, Carinhas N, Glassey J, Hesse F, Merten OW, Micheletti M, Noll T, Oliveira R, Reichl U, Staby A, Teixeira AP, Weichert H, Mandenius CF, 2012. How can measurement, monitoring, modeling and control advance cell culture in industrial biotechnology? Biotechnol J, 7: 1522–1529. http://dx.doi.org/10.1002/biot.201200226

  35. Carinhas N, Oliveira R, Alves PM, Carrondo MJ, Teixeira AP, 2012. Systems biotechnology of animal cells: the road to prediction. Trends Biotechnol, 30(7):377-85 http://dx.doi.org/10.1016/j.tibtech.2012.03.004

  36. Almeida AS, Queiroga CS, Sousa MF, Alves PM, Vieira HL, 2012 Carbon monoxide modulates apoptosis by reinforcing oxidative metabolism in astrocytes: role of BCL-2. J Biol Chem, 287 (14): 10761-70. http://dx.doi.org/10.1074/jbc.M111.306738

  37. Queiroga CS, Tomasi S, Wideroe M, Alves PM, Vercelli A, Vieira HL, 2012. Preconditioning triggered by Carbon Monoxide (CO) provides neuronal protection following perinatal hypoxia-ischemia. Plos One, 7(8): e42632. http://dx.doi.org/10.1371/journal.pone.0042632

  38. Queiroga CS, Almeida AS and Vieira HL, 2012. Carbon monoxide targeting mitochondria. Biochem Res Int, 2012:749845. http://dx.doi.org/10.1155/2012/749845

  39. Tavares L, Figueira I, McDougall GJ, Vieira HL, Stewart D, Alves PM, Ferreira RB, Santos CN, 2012. Neuroprotective effects of digested polyphenols from wild blackberry species. Eur J Nutr. http://dx.doi.org/10.1007/s00394-012-0307-7

  40. Rodrigues AF, Amaral AI, Veríssimo V, Alves PM, Coroadinha AS, 2012. Adaptation of retrovirus producer cells to serum deprivation: Implications in lipid biosynthesis and vector production. Biotechnol Bioeng, 109 (5): 1269-79. http://dx.doi.org/10.1002/bit.24410

  41. Roldão A, Mellado MC, Lima JC, Carrondo MJ, Alves PM, Oliveira R, 2012. On the effect of thermodynamic equilibrium on the assembly efficiency of complex multi-layered virus-like particles (VLP): the case of rotavirus VLP. PLoS Comput Biol, 8(2): e1002367. http://dx.doi.org/10.1371/journal.pcbi.1002367

  42. Teixeira AP, Duarte TM, Carrondo MJ, Alves PM, 2011. Synchronous fluorescence spectroscopy as a novel tool to enable PAT applications in bioprocesses. Biotechnol Bioeng, 108(8):1852-61. http://dx.doi.org/10.1002/bit.23131

  43. Tostões RM, Leite SB, Miranda JP, Sousa MF, Wang DIC, Carrondo MJ, Alves PM, 2011. Perfusion of 3D encapsulated hepatocytes-a synergistic effect enhancing long-term functionality in bioreactors. Biotechnol Bioeng, 108: 41-49. http://dx.doi.org/10.1002/bit.22920

  44. Rodrigues AF, Guerreiro MR, Santiago VM, Dalba C, Klatzmann D, Alves PM, Carrondo MJ, Coroadinha AS, 2011. Down-regulation of CD81 tetraspanin in human cells producing retroviral-based particles: tailoring vector composition. Biotechnol Bioeng, 108(11):2623-33. http://dx.doi.org/10.1002/bit.23231

  45. Carinhas N, Robitaille AM, Moes S, Carrondo MJ, Jenoe P, Oliveira R, Alves PM, 2011. Quantitative proteomics of Spodoptera frugiperda cells during growth and baculovirus infection. Plos One, 6(10):e26444. http://dx.doi.org/10.1371/journal.pone.0026444

  46. Queiroga CS, Almeida AS, Alves PM, Brenner C, Vieira HL, 2011. Carbon monoxide prevents hepatic mitochondrial membrane permeabilization. BMC Cell Biol 12:10. http://dx.doi.org/10.1186/1471-2121-12-10

  47. Teixeira AP, Dias JM, Carinhas N, Sousa MF, Clemente JJ, Cunha AE, Von Stosch M, Alves PM, Carrondo MJ, Oliveira R, 2011. Cell functional enviromics: unravelling the function of environmental factors. BMC Syst Biol, 5:92. http://dx.doi.org/10.1186/1752-0509-5-92

  48. Carinhas N, Bernal V, Teixeira AP, Carrondo MJ, Alves PM, Oliveira R, 2011. Hybrid metabolic flux analysis: combining stoichiometric and statistical constraints to model the formation of complex recombinant products. BMC Syst Biol, 5:34. http://dx.doi.org/10.1186/1752-0509-5-34

  49. Teixeira AP, Duarte TM, Oliveira R, Carrondo MJ, Alves PM, 2011. High-throughput analysis of animal cell cultures using two dimensional fluorometry. J Biotechnol, 151:255-60. http://dx.doi.org/10.1016/j.jbiotec.2010.11.015

  50. Roldão A, Vicente T, Peixoto C, Carrondo MJ, Alves PM, 2011. Quality control and analytical methods for baculovirus based products. J Invertebr Pathol, 107:S94-S105. http://dx.doi.org/10.1016/j.jip.2011.05.009

  51. Carvalhal AV, Santos SS, Carrondo MJ, 2011. Extracellular purine and pyrimidine catabolism in cell culture. Biotechnol Prog, 27:1373-82. http://dx.doi.org/10.1002/btpr.656

  52. Carinhas N, Bernal V, Monteiro F, Carrondo MJ, Oliveira R, Alves PM, 2010. Improving baculovirus production at high cell density through manipulation of energy metabolism. Metabol Eng, 12:39-52. http://dx.doi.org/10.1016/j.ymben.2009.08.008

  53. Queiroga CS, Almeida AS, Martel C, Brenner C, Alves PM, Vieira HL, 2010. Glutathionylation of adenine nucleotide translocase induced by carbon monoxide prevents mitochondrial membrane permeabilisation and apoptosis. J Biol Chem, 285(22):17077-17088. http://dx.doi.org/10.1074/jbc.M109.065052

  54. Gama-Norton L, Herrmann S, Schucht R, Coroadinha AS, Löw R, Alves PM, Bartholomae CC, Schmidt M, Baum C, Schambach A, Hauser H, Wirth D, 2010. Retroviral vector performance in defined chromosomal Loci of modular packaging cell lines. Hum Gene Ther, 21(8):979-91. http://dx.doi.org/10.1089/hum.2009.089

  55. Teixeira AP, Oliveira R, Alves P, Carrondo MJ, 2009. Advances in on-line monitoring and control of mammalian cell cultures: supporting the PAT initiative. Biotechnol Adv, 27:726-32. http://dx.doi.org/10.1016/j.biotechadv.2009.05.003

  56. Carinhas N, Bernal V, Yokomizo AY, Carrondo MJ, Oliveira R, Alves PM, 2009. Baculovirus production for gene therapy: the role of cell density, multiplicity of infection and medium exchange. Appl Microbiol Biotechnol, 81(6):1041-49. http://dx.doi.org/10.1007/s00253-008-1727-4

  57. Roldão A, Oliveira R, Carrondo MJ, Alves PM, 2009. Error assessment in recombinant baculovirus titration: evaluation of different methods. J Virol Methods, 159:69-80. http://dx.doi.org/10.1016/j.jviromet.2009.03.007

  58. Teixeira AP, Portugal CA, Carinhas N, Dias JM, Crespo JP, Alves PM, Carrondo MJ, Oliveira R, 2009. In situ 2D fluorometry and chemometric monitoring of mammalian cell cultures. Biotechnol Bioeng, 102(4):1098-106. http://dx.doi.org/10.1002/bit.22125

  59. Bernal V, Carinhas N, Yokomizo A, Carrondo MJ, Alves PM, 2009. Cell density effect in the Baculovirus-Insect cells system: a quantitative analysis of energetic metabolism. Biotechnol Bioeng, 104(1):162-80. http://dx.doi.org/10.1002/bit.22364

  60. Rodrigues AF, Carmo M, Alves PM, Coroadinha AS, 2009. Retroviral vector production under serum deprivation: the role of lipids. Biotechnol Bioeng, 104(6):1171-81. http://dx.doi.org/10.1002/bit.22499

  61. Teixeira AP, Santos SS, Carinhas N, Oliveira R, Alves PM, 2008. Combining metabolic flux analysis tools and 13C-NMR to estimate intracellular fluxes of cultured astrocytes. Neurochem Int, 52:478-86. http://dx.doi.org/10.1016/j.neuint.2007.08.007

  62. Roldão A, Carrondo MJ, Alves PM, Oliveira, R, 2008. Stochastic simulation of protein expression in the Baculovirus/Insect cell system. Comp Chem Eng, 32:68-77. http://dx.doi.org/10.1016/j.compchemeng.2007.04.017

  63. Vieira HL, Queiroga CS, Alves PM, 2008. Preconditioning induced by Carbon Monoxide provides neuronal protection against apoptosis. J Neurochem, 107:375-84. http://dx.doi.org/10.1111/j.1471-4159.2008.05610.x

  64. Clemente JJ, Monteiro SMS, Carrondo MJ, Cunha AE, 2008. Predicting sporulation events in a bioreactor using an Electronic Nose. Biotechnol Bioeng, 101(3):545-52. http://dx.doi.org/10.1002/bit.21920

  65. Teixeira A, Alves C, Alves PM, Carrondo MJ, Oliveira R, 2007. Hybrid elementary flux analysis/nonparametric modeling: application for bioprocess control. BMC Bioinformatics, 8(1):30. http://dx.doi.org/10.1186/1471-2105-8-30

  66. Roldão A, Vieira HL, Charpilienne A, Poncet D, Roy P, Carrondo MJ, Alves PM, Oliveira R, 2007. Modeling rotavirus-like particles production in a baculovirus expression vector system: infection kinetics, baculovirus DNA replication, mRNA synthesis and protein production. J Biotechnol, 128(4):875-94. http://dx.doi.org/10.1016/j.jbiotec.2007.01.003

  67. Teixeira AP, Carinhas N, Dias JM, Cruz PE, Alves PM, Carrondo MJ, Oliveira R, 2007. Hybrid semi-parametric mathematical systems: bridging the gap between biology and process engineering. J Biotechnol, 132:418-25. http://dx.doi.org/10.1016/j.jbiotec.2007.08.020

  68. Vieira HL, Pereira ACP, Carrondo MJ, Alves PM, 2006. Catalase effect on apoptosis for the improvement of recombinant protein production in baculovirus-insect cell system. Bioprocess Biosyst Eng, 29(5-6):409-14. http://dx.doi.org/10.1007/s00449-006-0093-9

  69. Roldão A, Vieira HL, Alves PM, Oliveira R, Carrondo MJ, 2006. Intracellular dynamics in Rotavirus-like particles production: Evaluation of multigene and monocistronic infection strategies. Proc Biochem, 41:2188-99 http://doi:10.1016/j.procbio.2006.06.019

  70. Teixeira AP, Clemente JJ, Cunha AE, Carrondo MJ, Oliveira R, 2006. Bioprocess iterative batch-to-batch optimization based on hybrid parametric/nonparametric models. Biotechnol Prog, 22(1):247-58. http://dx.doi.org/10.1021/bp0502328

  71. Teixeira A, Cunha AE, Clemente JJ, Moreira JL, Cruz HJ, Alves PM, Carrondo MJ, Oliveira R, 2005. Modelling and optimization of a recombinant BHK-21 cultivation process using hybrid grey-box systems. J Biotechnol, 118:290-303. http://dx.doi.org/10.1016/j.jbiotec.2005.04.024

  72. Oliveira R, Clemente JJ, Cunha AE, Carrondo MJ, 2005. Adaptive dissolved oxygen control through the glycerol feeding in a recombinant Pichia pastoris cultivation in conditions of oxygen transfer limitation. J Biotechnol, 116(1):35-50. http://dx.doi.org/10.1016/j.jbiotec.2004.09.016

  73. Monteiro SM, Clemente JJ, Henriques AO, Gomes RJ, Carrondo MJ, Cunha AE, 2005. A procedure for high-yield spore production by Bacillus subtilis. Biotechnol Prog, 21(4):1026-31. http://dx.doi.org/10.1021/bp050062z

  74. Kreij K, Mandenius CF, Clemente JJ, Cunha AE, Monteiro SM, Carrondo MJ, Hesse F, de Los Molinas MM, Wagner R, Merten OW, Gény-Fiamma C, Leger W, Wiesinger-Mayr H, Müller D, Katinger H, Mårtensson P, Bachinger T, Mitrovics J, 2005. On-line detection of microbial contaminations in animal cell reactor cultures using an electronic nose device. Cytotechnology, 48(1-3):41-58. http://dx.doi.org/10.1007/s10616-005-3587-4

  75. Maranga L, Cunha A, Clemente J, Cruz PE, Carrondo MJ, 2004. Scale-up of virus-like particles production: effects of sparging, agitation and bioreactor scale on cell growth, infection kinetics and productivity. J Biotechnol, 107:55-64. http://dx.doi.org/10.1016/j.jbiotec.2003.09.012

  76. Cunha AE, Clemente JJ, Gomes R, Pinto F, Thomaz M, Miranda S, Pinto R, Moosmayer D, Donner P, Carrondo MJ, 2004. Methanol induction optimization for scFv antibody fragment production in Pichia pastoris. Biotechnol Bioeng, 86(4):458-67. http://dx.doi.org/10.1002/bit.20051

  77. Carvalhal AV, Santos SS, Calado J, Haury M, Carrondo MJ, 2003. Cell growth arrest by nucleotides, nucleosides and bases as a tool for improved production of recombinant proteins. Biotechnol Prog, 19:69-83. http://dx.doi.org/10.1021/bp0255917

  78. Carvalhal AV, Marcelino I, Carrondo MJ, 2003. Metabolic changes during cell growth inhibition by p27 overexpression. Appl Microbiol Biotechnol, 63:164-73. http://dx.doi.org/10.1007/s00253-003-1385-5

  79. Maranga L, AS Coroadinha, MJ Carrondo, 2002. Insect cell culture medium supplementation with fetal bovine serum and bovine serum albumin: effects on baculovirus adsorption and infection kinetics. Biotechnol Prog, 18:855-61. http://dx.doi.org/10.1021/bp025514b

  80. Carvalhal A, Coroadinha AS, Alves PM, Moreira JL, Hauser H, Carrondo MJ, 2002. Metabolic changes during cell growth inhibition by the IRF-1 system. Enzyme Microb Technol, 30:95-109. http://dx.doi.org/10.1016/S0141-0229(01)00460-4

  81. Cruz PE, L Maranga, MJ Carrondo, 2002. Integrated process optimization: lessons from retrovirus and virus-like particle production. J Biotechnol, 99:199-214. http://dx.doi.org/10.1016/S0168-1656(02)00210-9

  82. Fonseca LL, Alves PM, Carrondo MJ, Santos H, 2001. Effect of ethanol on the metabolism of primary astrocytes studied by [13C]- and [31P]-NMR spectroscopy. J Neurosci Res, 66:803-11. http://dx.doi.org/10.1002/jnr.10039

  83. Carvalhal AV, Moreira JL, Carrondo MJ, 2001. Strategies to modulate BHK cell proliferation by the regulation of IRF-1 expression. J Biotechnol, 92:47-59. http://dx.doi.org/10.1016/S0168-1656(01)00365-0

  84. Merten OW, Cruz PE, Rochette C, Geny-Fiamma C, Bouquet C, Goncalves D, Danos O, Carrondo MJ, 2001. Comparison of different bioreactor systems for the production of high titer retroviral vectors. Biotechnol Prog, 17:326-35. http://dx.doi.org/10.1021/bp000162z

  85. Cruz HJ, Dias EM, Peixoto CM, Moreira JL, Carrondo MJ, 2000. Product quality of a recombinant fusion protein expressed in immobilised baby hamster cells grown in protein-free medium. Biotechnol Lett, 22:677-82. http://dx.doi.org/10.1023/A:1005631602517

  86. Cruz PE, Almeida JS, Murphy PN, Moreira JL, Carrondo MJ, 2000. Modeling retrovirus production for gene therapy. 1. Determination of optimal bioreaction mode and harvest strategy. Biotechnol Prog, 16:213-21. http://dx.doi.org/10.1021/bp9901466

  87. Cruz PE, Goncalves D, Almeida J, Moreira JL, Carrondo MJ, 2000. Modeling retrovirus production for gene therapy. 2. Integrated optimization of bioreaction and downstream processing. Biotechnol Prog, 16:350-57. http://dx.doi.org/10.1021/bp000017l

  88. Cruz HJ, Peixoto CM, Nimtz M, Alves PM, Dias EM, Moreira JL, Carrondo MJ, 2000. Metabolic shifts do not influence the glycosylation patterns of a recombinant fusion protein expressed in BHK cells. Biotechnol Bioeng, 69:129-39. http://dx.doi.org/10.1002/(SICI)1097-0290(20000720)69:2<129::AID-BIT2>3.0.CO;2-5

  89. Cruz HJ, Freitas CM, Alves PM, Moreira JL, Carrondo MJ, 2000. Effects of ammonia and lactate on growth, metabolism, and productivity of BHK cells. Enzyme Microb Technol, 27:45-52. http://dx.doi.org/10.1016/S0141-0229(00)00151-4

  90. Cruz HJ, Moreira JL, Carrondo MJ, 2000. Metabolically optimised BHK cell fed-batch cultures. J Biotechnol, 80:109-18. http://dx.doi.org/10.1016/S0168-1656(00)00254-6

  91. Carvalhal AV, Moreira JL, Cruz H, Mueller P, Hauser H, Carrondo MJ, 2000. Manipulation of culture conditions for BHK cell growth inhibition by IRF-1 activation. Cytechnology, 32:135-45. http://dx.doi.org/10.1023/A:1008139304964

  92. Cruz HJ, JL Moreira, MJ Carrondo, 1999. Metabolic shifts by nutrient manipulation in continuous cultures of BHK cells. Biotechnol Bioeng, 66:104-13. http://dx.doi.org/10.1002/(SICI)1097-0290(1999)66:2%3C104::AID-BIT3%3E3.3.CO;2-R

  93. Cruz PE, Martins PC, Alves PM, Peixoto CC, Santos H, Moreira JL, Carrondo MJ,  1999. Proteolytic activity in infected and non infected insect cells: degradation of HIV-1 Pr55gag particles. Biotechnol Bioeng, 65:133-43. http://dx.doi.org/10.1002/(SICI)1097-0290(19991020)65:2%3C133::AID-BIT2%3E3.0.CO;2-X

  94. Cruz HJ, Ferreira AS, Freitas CM, Moreira JL, Carrondo MJ, 1999. Metabolic responses to different glucose and glutamine levels in baby hamster kidney cell culture. Appl Microbiol Biotechnol, 51: 579-85. http://dx.doi.org/10.1007/s002530051435

  95. Burger C, Carrondo MJ, Cruz H, Cuffe M, Dias E, Griffiths JB, Hayes K, Hauser H, Looby D, Mielke C, Moreira JL, Rieke E, Savage AV, Stacey GN, Welge T, 1999. An integrated strategy for the process development of a recombinant antibody-cytokine fusion protein expressed in BHK cells. Appl Microbiol Biotechnol, 52:345-53. http://dx.doi.org/10.1007/s002530051530

  96. Cruz HJ, Moreira JL, Stacey G, Dias EM, Hayes K, Looby D, Griffiths B, Carrondo MJ, 1998. Adaptation of BHK cells producing a recombinant protein to serum-free media and protein-free medium. Cytotechnology, 26:59-64. http://dx.doi.org/10.1023/A:1007951813755

  97. Cruz PE, Peixoto CC, Moreira JL, Carrondo MJ, 1998. Optimization of the production of virus-like particles in insect cells. Biotechnol Bioeng, 60:408-18. http://dx.doi.org/10.1002/(SICI)1097-0290(19981120)60:4<408::AID-BIT2>3.0.CO;2-Q

  98. Cruz PE, Peixoto CC, Moreira JL, Carrondo MJ, 1998. Production and quality analysis of Pr55gag particles produced in baculovirus-infected insect cells. J Chem Technol Biotechnol, 72:149-58. http://dx.doi.org/10.1002/(SICI)1097-4660(199806)72:2%3C149::AID-JCTB886%3E3.3.CO;2-3

  99. Kirchhoff S, Kröger A, Cruz H, Tümmler M, Schaper F, Köster M, Hauser H, 1996. Regulation of cell growth by IRF-1 in BHK-21 cells. Cytotechnology, 22:147-56. http://dx.doi.org/10.1007/BF00353934

  100. Alves PM, JL Moreira, JM Rodrigues, JG Aunins, MJ Carrondo, 1996. Two-dimensional versus three-dimensional culture systems: effects on growth and productivity of BHK cells. Biotechnol Bioeng, 52:429-32. http://dx.doi.org/10.1002/(SICI)1097-0290(19961105)52:3<429::AID-BIT9>3.0.CO;2-M

  101. Moreira JL, Santana PC, Feliciano AS, Cruz PE, Racher AJ, Griffiths JB, Carrondo MJ, 1995. Effect of viscosity upon hydrodynamically controlled natural aggregates of animal cells grown in stirred vessels. Biotechnol Prog, 11:575-83. http://dx.doi.org/10.1021/bp00035a012

  102. Moreira JL, Cruz PE, Santana P, Aunins JG, Carrondo MJ, 1995. Formation and disruption of animal cell aggregates in stirred vessels: mechanisms and kinetic studies. Chem Eng Sci, 50:2747-64. http://dx.doi.org/10.1016/0009-2509(95)00118-O

  103. Xavier AL, Gonçalves J, Moreira JL, Carrondo MJ, 1995. Operational patterns affecting lactic acid production in ultrafiltration cell recycle bioreactor. Biotechnol Bioeng, 45:320-27. http://dx.doi.org/10.1002/bit.260450406

  104. Moreira JL, Alves PM, Aunins JG, Carrondo MJ, 1995. Hydrodynamic effects on BHK cells grown as suspended natural aggregates. Biotechnol Bioeng, 46:351-60. http://dx.doi.org/10.1002/bit.260460408

  105. Moreira JL, Alves PM, Feliciano A, Aunins JG, Carrondo MJ, 1995. Serum-free and serum-containing media for growth of suspended BHK aggregates in stirred vessels. Enzyme Microb Tech, 17:437-44. http://dx.doi.org/10.1016/0141-0229(94)00071-X

  106. Almeida JS, Reis MA, Carrondo MJ, 1995. Competition between nitrate and nitrite reduction in denitrification by Pseudomonas fluorescens. Biotechnol Bioeng, 46(5):476-84. http://dx.doi.org/10.1002/bit.260460512

  107. Almeida JS, Júlio SM, Reis MA, Carrondo MJ, 1995. Nitrite inhibition of denitrification by Pseudomonas fluorescens. Biotechnol Bioeng, 46(3):194-201. http://dx.doi.org/10.1002/bit.260460303

  108. Racher AJ, Moreira JL, Alves PM, Wirth M, Weidle UH, Hauser H, Carrondo MJ, Griffiths JB, 1994. Expression of recombinant antibody and secreted alkaline phosphatase in mammalian cells. Influence of cell line and culture system upon production kinetics. Appl Microbiol Biotechnol, 40:851-6. http://dx.doi.org/10.1007/BF00173987

  109. Moreira JL, Alves PM, Aunins JG, Carrondo MJ, 1994. Changes in animal cell natural aggregates in suspended batch cultures. Appl Microbiol Biotechnol, 41: 203-9. http://dx.doi.org/10.1007/BF00186960

  110. Moreira JL, Feliciano AS, Santana PC, Cruz PE, Aunins JG, Carrondo MJ, 1994. Repeated-batch cultures of baby hamster kidney cell aggregates in stirred vessels. Cytotechnology, 15:337-49. http://dx.doi.org/10.1007/BF00762409

  111. Moreira JL, Alves PM, Rodrigues JM, Cruz PE, Aunins JG, Carrondo MJ, 1994. Studies of baby hamster kidney natural cell aggregation in suspended batch cultures. Ann N Y Acad Sci, 745:122-33. http://dx.doi.org/10.1111/j.1749-6632.1994.tb44368.x

  112. Moreira JL, Wirth M, Fitzek M, Hauser H, 1992. Evaluation of reporter genes in mammalian cell lines. Meth Mol Cell Biol, 3:23-2.

  113. Reis MA, Almeida JS, Lemos PC, Carrondo MJ, 1992. Effect of hydrogen sulfide on growth of sulfate reducing bacteria. Biotechnol Bioeng, 40(5):593-600. http://dx.doi.org/10.1002/bit.260400506

  114. Crespo JP, Almeida JS, Moura MJ, Carrondo MJ, 1990. Modeling of immobilized cell reactor for propionic acid fermentation. Biotechnol Bioeng, 36(7):705-16. http://dx.doi.org/10.1002/bit.260360708

  115. Carrondo MJ, Reboredo F, Ganho RM, Oliveira JF, 1984. Analysis of sediments for heavy metals by a rapid electrothermal atomic-absorption procedure. Talanta, 31(7):561-4. http://dx.doi.org/10.1016/0039-9140(84)80141-1

  116. Carrondo MJ, Lester JN, Perry R, 1979. An investigation of a flameless atomic-absorption method for determination of aluminium, calcium, iron and magnesium in sewage sludge. Talanta, 26(10):929-33. http://dx.doi.org/10.1016/0039-9140(79)80128-9

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