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Cell Therapy/Stem Cell Engineering

  1. Almeida JI, Tenreiro MF, Martinez-Santamaria L, Aspizua SG, Gisbert JP, Alves PM, Serra M, Baptista PM. 2021. Hallmarks of the human intestinal microbiome on liver maturation and function. doi:10.1016/j.jhep.2021.10.015

  2. Sebastião MJ, Serra M, Gomes-Alves P, Alves PM. 2021. Stem cells characterization: OMICS reinforcing analytics. Current Opinion in Biotechnology. 20;71:175-181. doi: 10.1016/j.copbio.2021.07.021

  3. Tenreiro MF, Louro AF, Alves PM, Serra M. 2021. Next generation of heart regenerative therapies: progress and promise of cardiac tissue engineering. npj regenerative medicine. 6:30 (2021). doi: 10.1038/s41536-021-00140-4

  4. Sebastião MJ, Marcos-Silva L, Gomes-Alves P, Alves PM. 2021. Proteomic and Glyco(proteo)mic tools in the profiling of cardiac progenitors and pluripotent stem cell derived cardiomyocytes: Accelerating translation into therapy. Biotechnology Advances. doi:10.1016/j.biotechadv.2021.107755

  5. Isidro IA, Vicente P, Pais DAM, Almeida JI, Domingues M, Abecasis B, Zapata-Linares N, Rodriguez-Madoz JR, Prosper F, Aspegren A, Alves PM, Serra M. 2021. Online monitoring of hiPSC expansion and hepatic differentiation in 3D culture by dielectric spectroscopy. Biotechnology & Bioengineering. doi:10.1002/bit.27751

  6. Abecasis B, Canhão PGM, Almeida HV, Calmeiro T, Fortunato E, Gomes-Alves P, Serra M, Alves PM. 2020. Towards a microencapsulated 3D hiPSC-derived in vitro cardiac microtissue for recapitulation of human heart microenvironment features. Frontiers in Bioengineering and Biotechnology. 8:580744. doi:10.3389/fbioe.2020.580744

  7. Vasconcelos e Sá J, Simão D, Terrasso AP, Silva MM, Brito C, Isidro IA, Alves PM, Carrondo MJT. 2020. Unveiling dynamic metabolic signatures in human induced pluripotent and neural stem cells. PLOS Computational Biology. 16(4): e1007780. doi:10.1371/journal.pcbi.1007780

  8. Abecasis B, Gomes-Alves P, Rosa S, Gouveia PJ, Ferreira LS, Serra M, Alves PM. 2019. Unveiling the molecular crosstalk in a human induced pluripotent stem cell derived cardiac model. Biotechnol Bioeng. https://doi.org/10.1002/bit.26929

  9. Torán JL, López JA, Gomes-Alves P, Aguilar S, Torroja C, Trevisan-Herraz M, Moscoso I, Sebastião MJ, Serra M, Brito C, Cruz FM, Sepúlveda JC, Abad JL, Galán-Arriola C, Ibanez B, Martínez F, Fernández ME, Fernández-Aviles F, Palacios I, R-Borlado L, Vázquez J, Alves PM, Bernad A. 2019. Definition of a cell surface signature for human cardiac progenitor cells after comprehensive comparative transcriptomic and proteomic characterization. Sci Rep. Mar 15;9(1):4647. https://doi.org/10.1038/s41598-019-39571-x

  10. Sebastião MJ, Serra M, Pereira R, Palacios I, Gomes-Alves P, Alves PM. 2019. Human cardiac progenitor cell activation and regeneration mechanisms: exploring a novel myocardial ischemia/reperfusion in vitro model. Stem Cell Res Ther. Mar 7;10(1):77. https://doi.org/10.1186/s13287-019-1174-4 

  11. Rego D, Redondo L, Casaleiro S, Sousa AP, Abreu S, Serra M, Espirito Santo V. 2019. Application of pulsed electric fields for the valorization of platelets with no therapeutic value for transfusion medicine. Technology 7:40-45. https://doi.org/10.1142/S2339547819500031

  12. Correia C, Koshkin A, Duarte P, et al. 2018. 3D aggregate culture improves metabolic maturation of human pluripotent stem cell derived cardiomyocytes. Biotechnol Bioeng. 115(3):630-644. https://doi.org/10.1002/bit.26504

  13. Sebastião MJ, Pereira R, Serra M, Gomes-Alves P, Alves PM. 2018. Unveiling Human Cardiac Fibroblast Membrane Proteome. Proteomics.18(13):e1700446. https://doi.org/10.1002/pmic.201700446

  14. Simão D, Silva MM, Terrasso AP, et al. 2018. Recapitulation of Human Neural Microenvironment Signatures in iPSC-Derived NPC 3D Differentiation. Stem cell reports.11(2):552-564. https://doi.org/10.1016/j.stemcr.2018.06.020.

  15. Samaras JJ, Abecasis B, Serra M, Ducci A, Micheletti M. 2018. Impact of Hydrodynamics on iPSC-derived Cardiomyocyte Differentiation Processes. J Biotechnol. 287(March):18-27. https://doi.org/10.1016/j.jbiotec.2018.07.028.

  16. Sebastião MJ, Menta R, Serra M, et al. 2018. Human cardiac stem cells inhibit lymphocyte proliferation through paracrine mechanisms that correlate with indoleamine 2,3-dioxygenase induction and activity. Stem Cell Res Ther. 2(290):1-7. https://doi.org/10.1186/s13287-018-1010-2

  17. Hu D, Linders A, Yamak A, et al. 2018. Metabolic Maturation of Human Pluripotent Stem Cell Derived Cardiomyocytes by Inhibition of HIF1α and LDHA Basic Science Research Cell Signaling/Signal Transduction Contractile Function Metabolism Stem Cells. 1066-1079. https://doi.org/10.1161/CIRCRESAHA.118.313249.

  18. Serra M, Cunha B, Peixoto C, Gomes-Alves P, Alves PM. 2018. Advancing Manufacture of hMSC Therapies: technological challenges in cell bioprocessing and characterization. Current Opinion in Chemical Engineering. 22: 226-235 https://doi.org/10.1016/j.coche.2018.11.003

  19. Ana Teresa Serra, Margarida Serra, Ana Carina Silva, et al. 2018. Scalable culture strategies for the expansion of patient-derived cancer stem cell lines Stem Cells International. Stem Cells International https://doi.org/10.1155/2019/8347595

  20. Cunha B, Aguiar T, Carvalho SB, Silva MM, Gomes RA, Carrondo MJT, Gomes-Alves P, Peixoto C, Serra M, Alves PM. 2017. Bioprocess integration for human mesenchymal stem cells: from up to downstream processing scale-up to cell proteome characterization.  J Biotechnol. S0168-1656(17)30031-7.  https://doi.org/10.1016/j.jbiotec.2017.01.014

  21. Abecasis B, Aguiar T, Arnault E, Costa R, Gomes-Alves P, Aspegren A, Serra M, Alves PM. 2017. Expansion of 3D human induced pluripotent stem cell aggregates in bioreactors: Bioprocess intensification and scaling-up approaches.  J Biotechnol. 246:81-93. http://dx.doi.org/10.1016/j.jbiotec.2017.01.004

  22. Sá JV, Kleiderman S, Brito C, Sonnewald U, Leist M, Teixeira AP, Alves PM.  2016.  Quantification of Metabolic Rearrangements During Neural Stem Cells Differentiation into Astrocytes by Metabolic Flux Analysis.  Neurochemical Research.  ahead of print .  http://dx.doi.org/10.1007/s11064-016-1907-z

  23. Gouveia PJ, Rosa S, Ricotti L, Abecasis B, Almeida HV, Monteiro L, Nunes J, Carvalho FS, Serra M, Luchkin S, Kholkin AL, Alves PM, Oliveira PJ, Carvalho R, Menciassi A, das Neves RP, Ferreira LS. 2017. Flexible nanofilms coated with aligned piezoelectric microfibers preserve the contractility of cardiomyocytes. Biomaterials. 139:213-228. https://doi.org/10.1016/j.biomaterials.2017.05.048

  24. Santo VE, Rebelo SP, Estrada MF, Alves PM, Boghaert E, Brito C. 2017. Drug screening in 3D in vitro tumor models: overcoming current pitfalls of efficacy read-outs.  Biotechnology Journal. 1600505. http://dx.doi.org/10.1002/biot.201600505    

  25. Gomes-Alves P, Serra M, Brito C, Ricardo CP, Cunha R, Sousa MF, Sanchez B, Bernad A, Carrondo MJT, Rodriguez-Borlado L, Alves PM. 2016. In vitro expansion of human cardiac progenitor cells: exploring 'omics tools for characterization of cell-based allogeneic products Translational Research. http://www.sciencedirect.com/science/article/pii/S1931524416000554

  26. Correia C, Koshkin A, Carido M, Espinha N, Šarić T, Lima PA, Serra M, Alves PM. 2016. Effective Hypothermic Storage of Human Pluripotent Stem Cell-Derived Cardiomyocytes Compatible With Global Distribution of Cells for Clinical Applications and Toxicology Testing. Stem Cells Transl Med. http://stemcellstm.alphamedpress.org/content/early/2016/03/25/sctm.2015-0238.full.pdf

  27. Cunha B, Silva RJ, Aguiar T, Serra M, Daicic J, Maloisel JL, Clachan J, Åkerblom A, Carrondo MJ, Peixoto C, Alves PM. 2016. Improving washing strategies of human mesenchymal stem cells using negative mode expanded bed chromatography. J Chromatogr A. 2016 Jan 15;1429:292-303. http://dx.doi.org/10.1016/j.chroma.2015.12.052

  28. Kleiderman S, Sá JV, Teixeira AP, Brito C, Gutbier S, Evje LG, Hadera MG, Glaab E, Henry M, Sachinidis A, Alves PM, Sonnewald U, Leist M. 2015. Functional and phenotypic differences of pure populations of stem cell-derived astrocytes and neuronal precursor cells. Glia. 2015 Dec 22. http://dx.doi.org/10.1002/glia.22954

  29. Silva MM, Rodrigues AF, Correia C, Sousa MQF, Brito C, Coroadinha AS, Serra M, Alves PM. 2015. Robust expansion of human pluripotent stem cells: integration of bioprocess design with transcriptomic and metabolomic characterization. Stem Cells and Translational Medicine, in press.

  30. Cunha B, Aguiar T, Silva MM, Silva R, Sousa MF, Pineda E, Peixoto C, Carrondo MJ, Serra M, Alves PM. 2015. Exploring continuous and integrated strategies for the up- and downstream processing of human mesenchymal stem cells. Journal of Biotechnology - special issue on integrated continuous biomanufacturing, ahead of print 2015 Mar 4, http://dx.doi.org/10.1016/j.jbiotec.2015.02.023  

  31. Suter-Dick L, Alves PM, Blaauboer BJ, Bremm KD, Brito C, Coecke S, Flick B, Fowler P, Hescheler J, Ingelman-Sundberg M, Jennings P, Kelm JM, Manou I, Mistry P, Moretto A, Roth A, Stedman D, van de Water B, Beilmann M. 2015. Stem Cell-Derived Systems in Toxicology Assessment. Stem Cells Dev. Mar 31. [Epub ahead of print] http://dx.doi.org/10.1089/scd.2014.0540  

  32. Cunha B, Peixoto C, Silva MM, Carrondo MJT, Serra M, Alves PM, 2015

  33. Filtration methodologies for the clarification and concentration of human mesenchymal stem cells. J Memb Sci, Volume 478, 117-129. http://dx.doi.org/10.1016/j.memsci.2014.12.041

  34. Gomes-Alves, P., Serra, M., Brito, C., R-Borlado L, Lopez JA, Vazquez J, Carrondo MJT, Bernad A & Alves PM, 2015. Exploring analytical proteomics platforms toward the definition of human cardiac stem cells receptome. Proteomics 15, 1332-7. http://dx.doi.org/10.1002/pmic.20140031

  35. Correia C, Serra M, Espinha N, Sousa M, Brito C, Burkert K, Zheng Y, Hescheler J, Carrondo MJ, Sarić T, Alves PM. 2014. Combining Hypoxia and Bioreactor Hydrodynamics Boosts Induced Pluripotent Stem Cell Differentiation Towards Cardiomyocytes. Stem Cell Reviews and Reports. Published on line 15 July 2014. http://dx.doi.org/10.1007/s12015-014-9533-0

  36. Gualda EJ, Simão D, Pinto C, Alves PM, Brito C. 2014. Imaging of human differentiated 3D neural aggregates using light sheet fluorescence. Frontiers in Cellular Neuroscience. 8:221. http://dx.doi.org/10.3389/fncel.2014.00221

  37. Malpique R, Tostões R, Beier AF, Serra M, Brito C, Schulz JC, Björquist P, Zimmermann H, Alves PM, 2012. Surface-based cryopreservation strategies for human embryonic stem cells: A comparative study. Biotechnol Prog, 28(4):1079-87. http://dx.doi.org/10.1002/btpr.1572

  38. Serra M, Brito C, Correia C, Alves PM, 2012. Process engineering of human pluripotent stem cells for clinical applications. Trends Biotechnol, 30(6):350-9. http://dx.doi.org/10.1016/j.tibtech.2012.03.003

  39. Brito C, Simão D, Costa I, Malpique R, Pereira CI, Fernandes P, Serra M, Schwarz S, Schwarz J, Kremer EJ, Alves PM. 2012. “3D cultures of human neural progenitor cells: Dopaminergic differentiation and genetic modification”, Methods 56, 452–460. http://dx.doi.org/10.1016/j.ymeth.2012.03.005

  40. Serra M, Correia C, Malpique R, Brito C, Jensen J, Bjorquist P, Carrondo MJ, Alves PM, 2011. Microencapsulation technology: a powerful tool for integrating expansion and cryopreservation of human embryonic stem cells. Plos One, 6(8) e23212. http://dx.doi.org/10.1371/journal.pone.0023212

  41. Vieira HL, Alves PM, Vercelli A, 2011. Modulation of neuronal stem cell differentiation by hypoxia and reactive oxygen species. Prog Neurobiol, 93:444-55. http://dx.doi.org/10.1016/j.pneurobio.2011.01.007

  42. Serra M, Brito C, Sousa MF, Jensen J, Tostões RM, Clemente JJ, Hyllner J, Strehl R, Carrondo MJ, Alves PM, 2010. Improving expansion of pluripotent human embryonic stem cells in perfused bioreactors through oxygen control. J Biotechnol, 148(4):208-15. http://dx.doi.org/10.1016/j.jbiotec.2010.06.015

  43. Malpique R, Osório LM, Ferreira DS, Ehrhart F, Brito C, Zimmermann H, Alves PM, 2010. Alginate encapsulation as a novel strategy for the cryopreservation of neurospheres. Tissue Eng Part C Methods, 16(5):965-77. http://dx.doi.org/10.1089/ten.tec.2009.0660

  44. Malpique R, Ehrhart F, Katsen-Globa A, Zimmermann H, Alves PM, 2009. Cryopreservation of adherent cells: strategies to improve cell viability and function after thawing. Tissue Eng Part C Methods, 15(3):373-86. http://dx.doi.org/10.1089/ten.tec.2008.0410

  45. Serra M, Brito C, Costa E, Sousa MF, Alves PM, 2009. Integrating human stem cell expansion and neuronal differentiation in bioreactors. BMC Biotechnol, 9:82. http://dx.doi.org/10.1186/1472-6750-9-82

  46. Serra M, Brito C, Leite SB, Gorjup E, von Briesen H, Carrondo MJ, Alves PM, 2009. Stirred bioreactors for the expansion of adult pancreatic stem cells. Ann Anat, 191:104-15. http://dx.doi.org/10.1016/j.aanat.2008.09.005

  47. Malpique R, Katsen-Globa A, Carrondo MJ, Zimmermann H, Alves PM, 2007. Cryopreservation in micro-volumes: impact upon Caco-2 Colon adenocarcinoma cells proliferation and differentiation. Biotechnol Bioeng, 98:155-66. http://dx.doi.org/10.1002/bit.21382

  48. Serra M, Leite SB, Brito C, Costa J, Carrondo MJ, Alves PM, 2007. Novel culture strategy for human stem cell proliferation and neuronal differentiation. J Neurosci Res, 85(16):3557-66. http://dx.doi.org/10.1002/jnr.21451

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