Personal tools
You are here: Home / Labs / Animal Cell Technology Unit / Publications / Research articles by topic / Downstream Processing
 

Downstream Processing

  1. Moreira AS, Faria TQ, Oliveira JG, Kavara A, Schofield M, Sanderson T, Collins M, Gantier R, Alves PM, Carrondo MJT, Peixoto C. 2021. Enhancing the purification of Lentiviral vectors for clinical applications. Separation and Purification Technology. 274: 118598. doi:10.1016/j.seppur.2021.118598 

  2. Mendes JP, Silva RJS, Berg M, Mathiasson L, Peixoto C, Alves PM, Carrondo MJT. 2021. Oncolytic virus purification with periodic counter-current chromatography. Biotechnology and Bioengineering. 118:3522–3532. doi:10.1002/bit.27779

  3. Carvalho SB, Peixoto C, Carrondo MJT, Silva R. 2021. Downstream processing for influenza vaccines and candidates: an update. Biotechnology and Bioengineering. doi: 10.1002/bit.27803

  4. Moleirinho MG, Feast S, Moreira AS, Silva RJS, Alves PM, Carrondo MJT, Huber T, Fee C, Peixoto C. 2020. 3D-printed ordered bed structures for chromatographic purification of enveloped and non-enveloped viral particles. Separation and Purification Technology. 254:117681. doi:10.1016/j.seppur.2020.117681

  5. Moleirinho MG, Fernandes RP, Carvalho SB, Bezemer S, Detmers F, Hermans P, Silva RJS, Alves PM, Carrondo MJT, Peixoto C. 2020. Baculovirus affinity removal in viral-based bioprocesses. Separation and Purification Technology. 241: 116693. doi:10.1016/j.seppur.2020.116693

  6. Moleirinho MG, Silva RJS, Alves PM, Carrondo MJT, Peixoto C. 2019. Current challenges in biotherapeutic particles manufacturing. Expert Opinion on Biological Therapy. doi:10.1080/14712598.2020.1693541

  7. Moreira AS, Silva AC, Sousa MFQ, Hagner-McWhirterc A, Ahlénc G, Lundgren M, Coroadinha AS, Alves PM, Peixoto C, Carrondo MJT. 2020. Establishing Suspension Cell Cultures for Improved Manufacturing of Oncolytic Adenovirus. Biotechnol J. doi:10.1002/biot.201900411

  8. Silva RJS, Moleirinho MG, Moreira AS, Xenopoulos A, Alves PM, Carrondo MJT, Peixoto C. 2020. A Flow-Through Chromatographic Strategy for Hepatitis C Virus-Like Particles Purification. Processes. 8(1): 85. doi:10.3390/pr8010085

  9. Marchel M, Soares HR, Vormittag P, Hubbuch J, Coroadinha AS, Marrucho IM. 2019. High-throughput screening of aqueous biphasic systems with ionic liquids as additives for extraction and purification of enveloped virus-like particles. Engineering Reports. 1(1): e12030. doi:10.1002/eng2.12030

  10. Moleirinho MG, Silva RJS, Carrondo MJT, Alves PM, Peixoto C. 2019. Exosome-based therapeutics: Purification using semi-continuous multi-column chromatography. Separation and Purification Technology. 224:515-523. doi: 10.1016/j.seppur.2019.04.060

  11. Carvalho SB, Silva RJS, Moleirinho MG, Cunha B, Moreira AS, Xenopoulos A, Alves PM, Carrondo MJT, Peixoto C. 2019 Membrane‐Based Approach for the Downstream Processing of Influenza Virus‐Like Particles. Biotechnol J.  14(8): e1800570. https://doi.org/10.1002/biot.201800570

  12. Carvalho SB, Silva JS, Moreira AS, Cunha B, Clemente JJ, Alves PM, Carrondo MJT, Xenopoulos A, Peixoto C. 2019 Efficient filtration strategies for the clarification of influenza virus-like particles derived from insect cells. Separation and Purification Technology. 218: 81-88. https://doi.org/10.1016/j.seppur.2019.02.040

  13. Carvalho SB, Moreira AS, Gomes J, et al. 2018 A detection and quantification label-free tool to speed up downstream processing of model mucins. PLoS One. 13(1):e0190974. https://doi.org/10.1371/journal.pone.0190974

  14. Carvalho SB, Fortuna AR, Wolff MW, et al. 2018 Purification of influenza virus-like particles using sulfated cellulose membrane adsorbers. J Chem Technol Biotechnol. 93(7). https://doi.org/10.1002/jctb.5474

  15. Serra M, Cunha B, Peixoto C, Gomes-Alves P, Alves PM 2018 Advancing Manufacture of hMSC Therapies: technological challenges in cell bioprocessing and characterization. Current Opinion in Chemical Engineering.  22: 226-235. https://doi.org/10.1016/j.coche.2018.11.003

  16. Cunha B, Aguiar T, Carvalho SB, Silva MM, Gomes RA, Carrondo MJT, Gomes-Alves P, Peixoto C, Serra M, Alves PM. 2017. Bioprocess integration for human mesenchymal stem cells: from up to downstream processing scale-up to cell proteome characterization.  J Biotechnol. S0168-1656(17)30031-7.  https://doi.org/10.1016/j.jbiotec.2017.01.014

  17. Carvalho SB, Freire JM, Moleirinho MG, Monteiro F, Gaspar D, Castanho MA, Carrondo MJ, Alves PM, Bernardes GJ, Peixoto C. 2016. Bioorthogonal Strategy for Bioprocessing of Specific-Site-Functionalized Enveloped Influenza-Virus-Like Particles. Bioconjugate Chemistry. 2386–2399. https://doi.org/10.1021/acs.bioconjchem.6b00372

  18. Cunha B, Silva RJ, Aguiar T, Serra M, Daicic J, Maloisel JL, Clachan J, Åkerblom A, Carrondo MJ, Peixoto C, Alves PM. 2016. Improving washing strategies of human mesenchymal stem cells using negative mode expanded bed chromatography. J Chromatogr A. 2016 Jan 15;1429:292-303. http://dx.doi.org/10.1016/j.chroma.2015.12.052

  19. Silva R, Mota JP, Peixoto C, Alves PM, Carrondo MJT. 2015. Improving the downstream processing of vaccine and gene therapy vectors with continuous chromatography. Pharmaceutical Bioprocessing 3(8): 489-505. http://dx.doi.org/10.4155/pbp.15.29

  20. Nestola P, Peixoto C, Villain L, Alves PM, Carrondo MJT, Mota JPB. 2015. Rational development of two flowthrough purification strategies for adenovirus type 5 and retrovirus-like particles, Journal of Chromatography A. http://dx.doi.org/10.1016/j.chroma.2015.11.037

  21. Cunha B, Aguiar T, Silva MM, Silva R, Sousa MF, Pineda E, Peixoto C, Carrondo MJ, Serra M, Alves PM. 2015. Exploring continuous and integrated strategies for the up- and downstream processing of human mesenchymal stem cells. Journal of Biotechnology - special issue on integrated continuous biomanufacturing, ahead of print 2015 Mar 4, http://dx.doi.org/10.1016/j.jbiotec.2015.02.023  

  22. Cunha B, Peixoto C, Silva M, Carrondo MJT, Serra M, Alves PM 2015. Filtration methodologies for the clarification and concentration of human mesenchymal stem cells. Journal of Membrane Science, 478:117-129. http://dx.doi.org/10.1016/j.memsci.2014.12.041

  23. Nestola P, Peixoto C, Silva RJS, Alves PM, Mota JPB, Carrondo MJT 2015. Improved virus purification processes for vaccines and gene therapy. Biotechnology and Bioengineering, 112(5):843-57. http://dx.doi.org/10.1002/bit.25545

  24. Nestola P, Silva RJS, Peixoto C, Alves PM, Carrondo MJT, Mota JPB 2015. Robust design of adenovirus purification by two-column, simulated countercurrent, size-exclusion chromatography. Journal of Biotechnology; in press. http://dx.doi.org/10.1016/j.jbiotec.2015.01.030

  25. Nestola P, Martins DL, Peixoto C, Roederstein S, Schleuss T, Alves PM, Mota JPB, Carrondo MJ. 2014. Evaluation of Novel Large Cut-Off Ultrafiltration Membranes for Adenovirus Serotype 5 (Ad5) Concentration. PLoS ONE, 9(12):-115802. http://dx.doi.org/10.1371/journal.pone.0115802

  26. Vlaming M, van Duijn E, Dillingh MR, Brands R, Windhorst AD, Hendrikse NH, Bosgra S, Burggraaf J, de Koning MC, Fidder A, Mocking J, Sandman H, de Ligt R, Fabriek BO, Pasman WJ, Seinen W, Alves T, Carrondo M, Peixoto C, Peeters P, Vaes W. 2015. Microdosing of a carbon-14 labeled protein in healthy volunteers accurately predicts its pharmacokinetics at therapeutic dosages. Clin Pharmacol Ther. Apr13. http://dx.doi.org/10.1002/cpt.131

  27. Gonçalves CSM, Sousa M, Martins DL, Barroso T, Pina AS, Peixoto C, Ricardo A, Roque CA. 2015. Biobased Monoliths for Adenovirus Purification. ACS Appl. Mater. Interfaces, 7 (12), pp 6605–6612. http://dx.doi.org/10.1021/am508907b

  28. Nestola P, Silva RJS, Peixoto C, Alves PM, Carrondo MJT, Mota JPB. 2014. Adenovirus purification by two-column, size-exclusion, simulated countercurrent chromatography. Journal of Chromatography A,1347: 111–121. http://dx.doi.org/10.1016/j.chroma.2014.04.079  

  29. Nestola P, Villain L, Peixoto C, Martins DL, Alves PM, Carrondo MJT, Mota JPB. 2014. Impact of grafting on the design of new membrane adsorbers for adenovirus purification. Journal of Biotechnology, 181: 1–11. http://dx.doi.org/10.1016/j.jbiotec.2014.04.003

  30. Fernandes P, Peixoto C, Santiago VM, Kremer EJ, Coroadinha AS, Alves PM, 2013. Bioprocess development for canine adenovirus type 2 vectors. Gene Ther. Apr;20(4):353-60. http://dx.doi.org/10.1038/gt.2012.52

  31. Bandeira V, Peixoto C, Rodrigues AF, Cruz PE, Alves PM, Coroadinha AS, Carrondo MJ, 2012. Downstream processing of lentiviral vectors: releasing bottlenecks. Hum Gene Ther Methods, 23(4):255-63. http://dx.doi.org/10.1089/hgtb.2012.059

  32. Vicente T, Mota JP, Peixoto C, Alves PM, Carrondo MJ, 2011. Rational design and optimization of downstream processes of virus particles for biopharmaceutical applications: current advances. Biotechnol Adv, 29: 869-78. http://dx.doi.org/10.1016/j.biotechadv.2011.07.004

  33. Lesch HP, Laitinen A, Peixoto C, Vicente T, Makkonen ME, Laitinen L, Pikkarainen JT, Samaranayake H, Alves PM, Carrondo MJ, Ylä-Herttuala S, Airenne KJ, 2011. Production and purification of lentiviral vectors generated in 293T suspension cells with baculoviral vectors. Gene Ther, 18:531-38. http://dx.doi.org/10.1038/gt.2010.162

  34. Vicente T, Roldão A, Peixoto C, Carrondo MJ, Alves PM, 2011. Large-scale production and purification of VLP-based vaccines. J Invertebr Pathol, 107:S42-S48. http://dx.doi.org/10.1016/j.jip.2011.05.004

  35. Vicente T, Fáber R, Alves PM, Carrondo MJ, Mota JP, 2011. Impact of ligand density on the optimization of ion-exchange membrane chromatography for viral vector purification. Biotechnol Bioeng, 108(6):1347-59. http://dx.doi.org/10.1002/bit.23058

  36. Roldão A, Vicente T, Peixoto C, Carrondo MJ, Alves PM, 2011. Quality control and analytical methods for baculovirus based products. J Invertebr Pathol, 107:S94-S105. http://dx.doi.org/10.1016/j.jip.2011.05.009

  37. Vicente T, Mota JP, Peixoto C, Alves PM, Carrondo MJ, 2010. Analysis of adsorption of a baculovirus bioreaction bulk on an ion-exchange surface by surface plasmon resonance. J Biotechnol, 148(4):171-81. http://dx.doi.org/10.1016/j.jbiotec.2010.05.005

  38. Vicente T, Mota JP, Peixoto C, Alves PM, Carrondo MJ, 2010. Modeling protein binding and elution over a chromatographic surface probed by surface plasmon resonance. J Chromatogr A, 1217:2032-41. http://dx.doi.org/10.1016/j.chroma.2010.01.068

  39. Vicente T, Peixoto C, Alves PM, Carrondo MJ, 2010. Modeling electrostatic interactions of baculovirus vectors for ion-exchange process development. J Chromatogr A, 1217:3754-64. http://dx.doi.org/10.1016/j.chroma.2010.03.059

  40. Vicente T, Peixoto C, Carrondo MJ, Alves PM, 2009. Purification of recombinant baculoviruses for gene therapy using membrane processes. Gene Ther, 16:766-775. http://dx.doi.org/10.1038/gt.2009.33

  41. Mellado MC, Peixoto C, Cruz PE, Carrondo MJ, Alves PM, 2008. Purification of recombinant rotavirus VP7 for the study of in vitro rotavirus-like particles assembly. J Chromatogr B, 874:89-94. http://dx.doi.org/10.1016/j.jchromb.2008.09.002

  42. Peixoto C, Ferreira TB, Sousa MF, Carrondo MJ, Alves PM, 2008. Towards purification of adenovirus vectors based on membrane technology. Biotechnol Prog, 24:1290-96. http://dx.doi.org/10.1002/btpr.25

  43. Rodrigues T, Alves A, Lopes A, Carrondo MJ, Alves PM, Cruz PE, 2008. Removal of envelope protein-free retroviral vectors by anion exchange chromatography to improve product quality. J Sep Sci, 31:3509-18. http://dx.doi.org/10.1002/jssc.200800195

  44. Mellado MC, Franco C, Coelho AV, AlvesPM, Simplício AL, 2008. Sodium dodecyl sulfate-capillary gel electrophoresis analysis of rotavirus-like particles. J Chromatogr A, 1192:166-72. http://dx.doi.org/10.1016/j.chroma.2008.03.035

  45. Vicente T, Sousa MF, Peixoto C, Mota JP, Alves PM, Carrondo MJ, 2008. Anion exchange membrane chromatography for purification of rotavirus-like particles. J Membr Sci, 311:270-83. http://dx.doi.org/10.1016/j.memsci.2007.12.021

  46. Peixoto C, Marcelino I, Amaral AI, Carrondo MJ, Alves PM, 2007. Purification by membrane technology of an intracellular Ehrlichia ruminantium candidate vaccine against heartwater. Proc Biochem, 42(7):1084-89. http://dx.doi.org/10.1016/j.procbio.2007.04.012

  47. Rodrigues T, Carvalho A, Carmo M, Carrondo MJ, Alves PM, Cruz PE, 2007. Scaleable purification process for gene therapy retroviral vectors. J Gene Med, 9(4):233-43. http://dx.doi.org/10.1002/jgm.1021

  48. Peixoto C, Sousa MF, Silva AC, Carrondo MJ, Alves PM, 2007. Downstream processing of triple-layered rotavirus like particles. J Biotechnol, 127:452-61 http://dx.doi.org/10.1016/j.jbiotec.2006.08.002

  49. Rodrigues T, Carrondo MJ, Alves PM, Cruz PE, 2007. Purification of retroviral vectors for clinical application: biological implication and technological challenges. J Biotechnol, 127:520-41. http://dx.doi.org/10.1016/j.jbiotec.2006.07.028

  50. Rodrigues T, Carvalho A, Roldao A, Carrondo MJ, Alves PM, Cruz PE, 2006. Screening anion-exchange chromatographic matrices for isolation of onco-retroviral vectors. J Chromatogr B, 837(1-2):59-68. http://dx.doi.org/10.1016/j.jchromb.2006.03.061

  51. Peixoto C, Ferreira TB, Carrondo MJ, Cruz PE, Alves PM, 2006. Purification of adenoviral vectors using expanded bed chromatography. J Virol Methods, 132:121-6. http://dx.doi.org/10.1016/j.jviromet.2005.10.002

  52. Peixoto CC, Marcelino I, Vachiéry N, Bensaid A, Martinez D, Carrondo MJ, Alves PM, 2005. Quantification of Ehrlichia ruminantium by real time PCR. Vet Microbiol, 107:273-8. http://dx.doi.org/10.1016/j.vetmic.2005.02.001

  53. Souza AP, Peixoto CC, Maranga L, Carvalhal AV, Moraes RH, Mendonça RZ, Pereira CA, Carrondo MJ, Mendonça RZ, 2005. Purification and characterization of an anti-apoptotic protein isolated from Lonomia obliqua Hemolymph. Biotechnol Prog, 21:99-105. http://dx.doi.org/10.1021/bp049831p

  54. Carmo M,  Peixoto CC, Coroadinha AS, Alves PM, Cruz PE, Carrondo MJ, 2004. Quantitation of MLV-based retroviral vectors using real-time RT-PCR. J Virol Methods, 119:115-9. http://dx.doi.org/10.1016/j.jviromet.2004.03.007

  55. Maranga L, Rueda P, Antonis AF, Vela C, Langeveld JP, Casal JI, Carrondo MJ, 2002. Large scale production and downstream processing of a recombinant porcine parvovirus vaccine. Appl Microbiol Biotechnol, 59:45-50. http://dx.doi.org/10.1007/s00253-002-0976-x

  56. Cruz PE, Peixoto CC, Devos K, Moreira JL, Saman E, Carrondo MJ, 2000. Characterization and downstream processing of HIV-1 core and virus-like-particles produced in serum free medium. Enzyme Microb Technol, 26:61-70. http://dx.doi.org/10.1016/S0141-0229(99)00128-3

 

Document Actions