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Vaccines

  1. Dias MM, Vidigal J, Sequeira DP, Alves PM, Teixeira AP, Roldão A. 2021. Insect High FiveTM cell line development using site-specific flipase recombination technology. G3 Genes|Genomes|Genetics. doi: 10.1093/g3journal/jkab166

  2. Fernandes B, Correia R, Sousa M, Carrondo MJT, Roldão A. 2021. Integrating high cell density cultures with adapted laboratory evolution for improved Gag-HA virus-like particles production in stable insect cell lines. Biotechnology and Bioengineering. doi: 10.1002/bit.27766

  3. Correia R, Meneses L, Richheimer C, Alves PM, Carrondo MJT, Duarte ARC, Paiva A, Roldão A. 2021. Improved storage of influenza HA-VLPs using a trehalose-glycerol natural deep eutectic solvent system. Vaccine. doi:10.1016/j.vaccine.2021.04.033

  4. Carvalho SB, Peixoto C, Carrondo MJT, Silva R. 2021. Downstream processing for influenza vaccines and candidates: an update. Biotechnology and Bioengineering. doi: 10.1002/bit.27803

  5. Correia R, Fernandes B, Alves PM, Carrondo MJT, Roldao A. 2020. Improving Influenza HA-Vlps Production in Insect High Five Cells via Adaptive Laboratory Evolution. Vaccines. 8(4), 589. doi:10.3390/vaccines8040589

  6. Guerreiro MR, Fernandes AR, Coroadinha AS. 2020. Evaluation of Structurally Distorted Split GFP Fluorescent Sensors for Cell-Based Detection of Viral Proteolytic Activity. Sensors. 21(1), 24. doi:10.3390/s21010024

  7. Moreira AS, Cavaco D, Faria T, Alves PM, Carrondo MJT, Peixoto C. 2020. Advances in  Lentivirus Purification. Biotechnology Journal. doi:10.1002/biot.202000019

  8. Pais DAM, Brown C, Neuman A, Mathur K, Isidro IA, Alves PM, Slade PG. 2020. Dielectric spectroscopy to improve the production of rAAV used in Gene Therapy. Processes. 8(11):1456. doi:10.3390/pr8111456

  9. Pais DA, Galrão PR, Kryzhanska A, Barbau J, Isidro IA, Alves PM. 2020. Holographic Imaging of Insect Cell Cultures: Online Non-Invasive Monitoring of Adeno-Associated Virus Production and Cell Concentration. Processes 8(4): 487. doi:10.3390/pr8040487

  10. Silva RJS, Moleirinho MG, Moreira AS, Xenopoulos A, Alves PM, Carrondo MJT, Peixoto C. 2020. A Flow-Through Chromatographic Strategy for Hepatitis C Virus-Like Particles Purification. Processes. 8(1): 85. doi:10.3390/pr8010085

  11. Marchel M, Soares HR, Vormittag P, Hubbuch J, Coroadinha AS, Marrucho IM. 2019. High-throughput screening of aqueous biphasic systems with ionic liquids as additives for extraction and purification of enveloped virus-like particles. Engineering Reports. 1(1): e12030. doi:10.1002/eng2.12030

  12. Suárez-Patiño SF, Bernardino TC, Núñez EGF, Astray RM, Pereira CA, Soares HR, Coroadinha AS, Jorge SAC. 2019. Semliki Forest Virus replicon particles production in serum-free medium BHK-21 cell cultures and their use to express different proteins. Cytotechnology. 71(5): 949-962. doi:10.1007/s10616-019-00337-y

  13. Fernandes B, Vidigal J, Correia R, Carrondo MJT, Alves PM, Teixeira AP, Roldão A. 2019. Adaptive laboratory evolution of stable insect cell lines for improved HIV-Gag VLPs production. J Biotechnol. doi:10.1016/j.jbiotec.2019.10.004

  14. Sousa M, Fenge C, Rupprecht J, Tappe A, Greller G, Alves P, Carrondo M, Roldão A. 2019. Process intensification for Peste des Petites Ruminants Virus vaccine production. Vaccine. https://doi.org/10.1016/j.vaccine.2019.07.009

  15. Soares HR, Ferreira-Fernandes M, Marchel M, Alves PM, Coroadinha AS. 2019. Enhancing Hepatitis C Virus pseudoparticles infectivity through p7NS2 cellular expression. J Virol Methods. 11:113714. https://doi.org/10.1016/j.jviromet.2019.113714​

  16. Carvalho SB, Silva RJS, Moleirinho MG, Cunha B, Moreira AS, Xenopoulos A, Alves PM, Carrondo MJT, Peixoto C. 2019. Membrane‐Based Approach for the Downstream Processing of Influenza Virus‐Like Particles. Biotechnol J. 14(8): e1800570. https://doi.org/10.1002/biot.201800570 ​

  17. Pais DAM, Portela RMC, Carrondo MJT, Isidro IA, Alves PM. 2019. Enabling PAT in insect cell bioprocesses: In situ monitoring of recombinant adeno-associated virus production by fluorescence spectroscopy. Biotechnol Bioeng. Jul 17 https://doi.org/10.1002/bit.27117

  18. Carvalho SB, Silva JS, Moreira AS, Cunha B, Clemente JJ, Alves PM, Carrondo MJT, Xenopoulos A, Peixoto C. 2019. Efficient filtration strategies for the clarification of influenza virus-like particles derived from insect cells. Separation and Purification Technology. 218: 81-88. doi: 10.1016/j.seppur.2019.02.040

  19. Charlton Hume HK, Vidigal J, Carrondo MJT, Middelberg APJ, Roldão A, Lua LHL. 2019. Synthetic biology for bioengineering virus-like particle vaccines. Biotechnol Bioeng. Apr;116(4):919-935. https://doi.org/10.1002/bit.26890

  20. Soares HR, Castro R, Tomás HA, Carrondo MJT, Alves PM, Coroadinha AS. 2019. Pseudotyping retrovirus like particles vaccine candidates with Hepatitis C virus envelope protein E2 requires the cellular expression of CD81. AMB Express. Feb 7;9(1):22. https://doi.org/10.1186/s13568-019-0741-5.​

  21. Vidigal J, Fernandes B, Dias MM, et al. 2018. RMCE-based insect cell platform to produce membrane proteins captured on HIV-1 Gag virus-like particles. Appl Microbiol Biotechnol.102(2):655-666. https://doi.org/10.1007/s00253-017-8628-3.​

  22. Sequeira DP, Correia R, Carrondo MJT, Roldão A, Teixeira AP, Alves PM. 2018. Combining stable insect cell lines with baculovirus-mediated expression for multi-HA influenza VLP production. Vaccine. 36(22):3112-3123. https://doi.org/10.1016/j.vaccine.2017.02.043.​

  23. Soares HR, Almeida AI, Tomás HA, Alves PM, Coroadinha AS. 2018. Flexible pseudotyping of retrovirus using recombinase-mediated cassette exchange. Biotechnol Lett. 40(4):633-639. https://doi.org/10.1007/s10529-018-2515-6.​

  24. Carvalho SB, Moreira AS, Gomes J, et al. 2018. A detection and quantification label-free tool to speed up downstream processing of model mucins. PLoS One. 13(1):e0190974. https://doi.org/10.1371/journal.pone.0190974.​

  25. Carvalho SB, Fortuna AR, Wolff MW, et al. 2017. Purification of influenza virus-like particles using sulfated cellulose membrane adsorbers. J Chem Technol Biotechnol. 93(7). https://doi.org/10.1002/jctb.5474

  26. Monteiro F, Bernal V, Alves PM. 2017. Combining stable insect cell lines with baculovirus-mediated expression for multi-HA influenza VLP production. BIOTECHNOLOGY AND BIOENGINEERING.  114, 674-684.  http://dx.doi.org/10.1002/bit.26089

  27. Sequeira DP, Correia R, Carrondo MJT, Roldao A, Teixeira AP, Alves PM.  2017.  Combining stable insect cell lines with baculovirus-mediated expression for multi-HA influenza VLP production.  Vaccine. pii: S0264-410X(17)30246-3. http://dx.doi.org/10.1016/j.vaccine.2017.02.043

  28. Patrone M, Coroadinha AS, Teixeira AP, Alves PM. 2016. Palmitoylation strengthens cholesterol-dependent multimerization and fusion activity of human cytomegalovirus gB. Journal of Biochemical Chemistry. http://dx.doi.org/10.1074/jbc.M115.682252

  29. Silva AC, Teixeira AP, Alves PM. 2016. Impact of Adenovirus infection in host cell metabolism evaluated by 1H NMR spectroscopy. J Biotechnol. http://dx.doi.org/10.1016/j.jbiotec.2016.05.025

  30. Soares HR, Castro R, Tomas HA, Rodrigues AF, Gomes-Alves P, Bellier B, Klatzmann D, Carrondo MJ, Alves PM, Coroadinha AS. 2016. Tetraspanins displayed in retrovirus-derived virus-like particles and their immunogenicity. Vaccine. http://dx.doi.org/10.1016/j.vaccine.2015.12.015

  31. Silva AC, Simão D, Sousa MFQ, Küppers C, Lucas T, Cruz P, Carrondo MJ,  Kochanek S, Alves PM 2015 Human Amniocyte-derived Cells for the Production of Adenoviral Vectors. Biotechnology Journal, http://dx.doi.org/10.1002/biot.201400765

  32. Castro R, Fernandes P, Laske T, Sousa MFQ, Genzel Y, Scharfenberg K, Alves PM, Coroadinha AS. 2015. Production of canine adenovirus type 2 in serum-free suspension cultures of MDCK cells. Appl Microbiol Biotechnol http://dx.doi.org/10.1007/s00253-015-6636-8

  33. Silva AC, Yami M, Libeau G, Carrondo MJ, Alves PM. 2014. Testing a new formulation for Peste des Petits Ruminants vaccine in Ethiopia. Vaccine, 32(24): 2878–81  http://dx.doi.org/10.1016/j.vaccine.2014.02.039 

  34. Patrone M, Carinhas N, Sousa MQ, Peixoto C, Ciferri C, Carfì A, Alves PM. 2014. Enhanced expression of full-length human cytomegalovirus fusion protein in non-swelling baculovirus-infected cells with a minimal fed-batch strategy. PloS One. http://dx.doi.org/10.1371/journal.pone.0090753

  35. Fernandes F, Dias MM, Vidigal J, Sousa MF, Patrone M, Teixeira AP, Alves PM. 2014. Production of rotavirus core-like particles in Sf9 cells using recombinase-mediated cassette exchange. J Biotechnol. http://dx.doi.org/10.1016/j.jbiotec.2013.11.020

  36. Fernandes F, Teixeira AP, Carinhas N, Carrondo MJT, Alves PM. 2013. Insect cells as production platform of complex VLPs. Expert Rev Vaccines. Feb;12(2):225-36.  http://dx.doi.org/10.1586/erv.12.153

  37. Marcelino I, Almeida AM, Brito C, Meyer D, Barreto M, Sheikboudou C, Franco CF, Martinez D, Lefrançois T, Vachiéry N, Carrondo MJ, Coelho A, Alves PM, 2012. Proteomic analyses of Ehrlichia ruminantium highlight differential expression of MAP1-family proteins. Vet Microbiol, 156 (2012) 305–314. http://dx.doi.org/10.1016/j.vetmic.2011.11.022

  38. Rodrigues AF, Guerreiro MR, Santiago VM, Dalba C, Klatzmann D, Alves PM, Carrondo MJ, Coroadinha AS, 2011. Down-regulation of CD81 tetraspanin in human cells producing retroviral-based particles: tailoring vector composition. Biotechnol Bioeng, 108(11):2623-33. http://dx.doi.org/10.1002/bit.23231

  39. Silva AC, Carrondo MJ, and Alves PM, 2011. Strategies for improved stability of Peste des Petits Ruminants vaccine. Vaccine, 29:4983-91. http://dx.doi.org/10.1016/j.vaccine.2011.04.102

  40. Roldão A, Mellado MC, Castilho LR, Carrondo MJ, Alves PM, 2010. Virus-like particles in vaccine development. Expert Rev Vaccines, 9(10):1149-76. http://dx.doi.org/10.1586/erv.10.115

  41. Matias AA, Serra AT, Silva AC, Perdigão R, Ferreira TB, Marcelino I, Silva S, Coelho AV, Alves PM, Duarte CM, 2010. Portuguese winemaking residues as a potential source of natural anti-adenoviral agents.Int. J Food Sci Nutr, 61(4):357-68. http://dx.doi.org/10.3109/09637480903430990

  42. Adakal H, Stachurski F, Konkobo M, Zoungrana S, Meyer DF, Pinarello V, Aprelon R, Marcelino I, Alves PM, Martinez D, Lefrancois T, Vachiéry N, 2010. Efficiency of inactivated vaccines against heartwater in Burkina Faso: Impact of Ehrlichia ruminantium genetic diversity. Vaccine, 28(29):4573-80. http://dx.doi.org/10.1016/j.vaccine.2010.04.087

  43. Mellado MC, Mena JA, Lopes A, Ramirez OT, Carrondo MJ, Palomares LA, Alves PM, 2009. Impact of physicochemical parameters on in vitro assembly and disassembly kinetics of recombinant triple-layered rotavirus-like particles. Biotechnol Bioeng, 104(4):674-86. http://dx.doi.org/10.1002/bit.22430

  44. Ferreira TB, Perdigão R, Silva AC, Zhang C, Aunins JG, Carrondo MJ, Alves PM, 2009. 293 cell cycle synchronisation in adenovirus vector production. Biotechnol Prog, 25(1):235-43. http://dx.doi.org/10.1002/btpr.64

  45. Mellado MC, Peixoto C, Cruz PE, Carrondo MJ, Alves PM, 2008. Purification of recombinant rotavirus VP7 for the study of in vitro rotavirus-like particles assembly. J Chromatogr B, 874:89-94. http://dx.doi.org/10.1016/j.jchromb.2008.09.002

  46. Roldão A, Carrondo MJ, Alves PM, Oliveira, R, 2008. Stochastic simulation of protein expression in the Baculovirus/Insect cell system. Comp Chem Eng, 32:68-77. http://dx.doi.org/10.1016/j.compchemeng.2007.04.017

  47. Silva AC, Delgado I, Sousa MF, Carrondo MJ, Alves PM, 2008. Scalable culture systems using different cell lines for the Production of Peste des Petits Ruminants Vaccine. Vaccine, 26:3305-11. http://dx.doi.org/10.1016/j.vaccine.2008.03.077

  48. Mellado MC, Franco C, Coelho AV, AlvesPM, Simplício AL, 2008. Sodium dodecyl sulfate-capillary gel electrophoresis analysis of rotavirus-like particles. J Chromatogr A, 1192:166-72. http://dx.doi.org/10.1016/j.chroma.2008.03.035

  49. Vicente T, Sousa MF, Peixoto C, Mota JP, Alves PM, Carrondo MJ, 2008. Anion exchange membrane chromatography for purification of rotavirus-like particles. J Membr Sci, 311:270-83. http://dx.doi.org/10.1016/j.memsci.2007.12.021

  50. Ferreira TB, Carrondo MJ, Alves PM, 2007. Effect of ammonia production on intracellular pH consequent effect on adenovirus vector production. J Biotechnol, 129(3):433-38. http://dx.doi.org/10.1016/j.jbiotec.2007.01.010

  51. Peixoto C, Sousa MF, Silva AC, Carrondo MJ, Alves PM, 2007. Downstream processing of triple-layered rotavirus like particles. J Biotechnol, 127:452-61 http://dx.doi.org/10.1016/j.jbiotec.2006.08.002

  52. Peixoto C, Marcelino I, Amaral AI, Carrondo MJ, Alves PM, 2007. Purification by membrane technology of an intracellular Ehrlichia ruminantium candidate vaccine against heartwater. Proc Biochem, 42(7):1084-89. http://dx.doi.org/10.1016/j.procbio.2007.04.012

  53. Roldão A, Vieira HL, Charpilienne A, Poncet D, Roy P, Carrondo MJ, Alves PM, Oliveira R, 2007. Modeling rotavirus-like particles production in a baculovirus expression vector system: infection kinetics, baculovirus DNA replication, mRNA synthesis and protein production. J Biotechnol, 128(4):875-94. http://dx.doi.org/10.1016/j.jbiotec.2007.01.003

  54. Faburay B, Geysen D, Ceesay A, Marcelino I, Alves PM, Taoufik A, Postigo M, Bell-Sakyi L, Jongejan F, 2007. Immunisation of sheep against heartwater in The Gambia using inactivated and attenuated Ehrlichia ruminantium vaccines. Vaccine, 25(46):7939-47. http://dx.doi.org/10.1016/j.vaccine.2007.09.002

  55. Marcelino I, Vachiéry N, Amaral AI, Roldão A, Lefrançois T, Carrondo MJ, Alves PM, Martinez D, 2007. Effect of the purification process and the storage conditions on the efficacy of an inactivated vaccine against heartwater. Vaccine, 25(26):4903-13. http://dx.doi.org/10.1016/j.vaccine.2007.04.055

  56. Lameiro MH, Malpique R, Silva AC, Alves PM, Melo E, 2006. Encapsulation of adenoviral vectors into chitosan-bile salt microparticles for mucosal vaccination, J Biotechnol, 126:152-62. http://dx.doi.org/10.1016/j.jbiotec.2006.04.030

  57. Marcelino I, Sousa MF, Veríssimo C, Cunha A, Carrondo MJ, Alves PM, 2006. Process development for the mass production of Ehrlichia ruminantium. Vaccine, 24(10):1716-25. http://dx.doi.org/10.1016/j.vaccine.2005.08.109

  58. Roldão A, Vieira HL, Alves PM, Oliveira R, Carrondo MJ, 2006. Intracellular dynamics in rotavirus-like particles production: Evaluation of multigene and monocistronic infection strategies. Proc Biochem, 41:2188-99. http://doi:10.1016/j.procbio.2006.06.019

  59. Peixoto C, Ferreira TB, Carrondo MJ, Cruz PE, Alves PM, 2006. Purification of adenoviral vectors using expanded bed chromatography. J Virol Methods, 132:121-6. http://dx.doi.org/10.1016/j.jviromet.2005.10.002

  60. Zhang C, Ferreira TB, Cruz PE, Alves PM, Haury M, Carrondo MJ, 2006. The importance of 293 cell cycle phase on adenovirus vector production, Enzyme Microb Technol. 39:1328-32. http://dx.doi.org/10.1016/j.enzmictec.2006.03.021

  61. Antonis AF, Bruschke CJ, Rueda P, Maranga L, Casal JI, Vela C, Hilgers LA, Belt PB, Weerdmeester K, Carrondo MJ, Langeveld JP, 2006. A novel recombinant virus-like particle vaccine for prevention of porcine parvovirus-induced reproductive failure. Vaccine, 24(26):5481-90. http://dx.doi.org/10.1016/j.vaccine.2006.03.089

  62. Ferreira TB, Ferreira AL, Carrondo MJ, Alves PM, 2005. Effect of refeed strategies and non-ammoniagenic medium on adenovirus production at high cell densities. J Biotechnol, 119:272-80. http://dx.doi.org/10.1016/j.jbiotec.2005.03.009

  63. Vieira HL, Estevão C, Roldão A, Peixoto CC, Sousa MF, Cruz PE, Carrondo MJ, Alves PM, 2005. Triple layered rotavirus VLP assembly: kinetics of vector replication, mRNA stability and recombinant protein production. J Biotechnol, 120(1):72-82. http://dx.doi.org/10.1016/j.jbiotec.2005.03.026

  64. Ferreira TB, Alves PM, Aunins JG, Carrondo MJ, 2005. Use of adenoviral vectors as veterinary vaccines. Gene Ther, 12(S1):73-83. http://dx.doi.org/10.1038/sj.gt.3302618

  65. Ferreira TB, Ferreira AL, Carrondo MJ, Alves PM, 2005. Two different serum-free media and osmolality effect upon human 293 cell growth and adenovirus production. Biotechnol Lett, 27(22): 1809-13. http://dx.doi.org/10.1007/s10529-005-3558-z

  66. Peixoto CC, Marcelino I, Vachiéry N, Bensaid A, Martinez D, Carrondo MJ, Alves PM, 2005. Quantification of Ehrlichia ruminantium by real time PCR. Vet Microbiol, 107:273-8. http://dx.doi.org/10.1016/j.vetmic.2005.02.001

  67. Marcelino I, Veríssimo C, Sousa MF, Carrondo MJ, Alves PM, 2005. Characterization of Ehrlichia ruminantium replication and release kinetics in endothelial cell cultures. Vet Microbiol, 110:87-96. http://dx.doi.org/10.1016/j.vetmic.2005.07.012

  68. Maranga L, Brazão TF, Carrondo MJ, 2003. Virus like particles production at low multiplicities of infection with the baculovirus insect cell system. Biotechnol  Bioeng, 84:245-53. http://dx.doi.org/10.1002/bit.10773

  69. Maranga L, Mendonca RZ, Bengala A, Peixoto CC, Moraes RH, Pereira CA, Carrondo MJ, 2003. Enhancement of Sf-9 cell growth and longevity through supplementation of culture medium with hemolymph. Biotechnol Prog, 19:58-63. http://dx.doi.org/10.1021/bp025583q

  70. Cruz PE, L Maranga, MJ Carrondo, 2002. Integrated process optimization: lessons from retrovirus and virus-like particle production. J Biotechnol, 99:199-214. http://dx.doi.org/10.1016/S0168-1656(02)00210-9

  71. Maranga L, Rueda P, Antonis AF, Vela C, Langeveld JP, Casal JI, Carrondo MJ, 2002. Large scale production and downstream processing of a recombinant porcine parvovirus vaccine. Appl Microbiol Biotechnol, 59:45-50. http://dx.doi.org/10.1007/s00253-002-0976-x

  72. Maranga L, PE Cruz, JG Aunins, MJ Carrondo, 2002. Production of core and virus-like particles with baculovirus infected insect cells. Adv Biochem Eng Biotechnol, 74:183-206. http://dx.doi.org/10.1007/3-540-45736-4_9

  73. Cruz PE, Peixoto CC, Devos K, Moreira JL, Saman E, Carrondo MJ, 2000. Characterization and downstream processing of HIV-1 core and virus-like-particles produced in serum free medium. Enzyme Microb Technol, 26:61-70. http://dx.doi.org/10.1016/S0141-0229(99)00128-3

  74. Cruz PE, Martins PC, Alves PM, Peixoto CC, Santos H, Moreira JL, Carrondo MJ, 1999. Proteolytic activity in infected and non infected insect cells: degradation of HIV-1 Pr55gag particles. Biotechnol Bioeng, 65:133-43. http://dx.doi.org/10.1002/(SICI)1097-0290(19991020)65:2%3C133::AID-BIT2%3E3.0.CO;2-X

  75. Cruz PE, Peixoto CC, Moreira JL, Carrondo MJ, 1998. Production and quality analysis of Pr55gag particles produced in baculovirus-infected insect cells. J Chem Technol Biotechnol, 72:149-58. http://dx.doi.org/10.1002/(SICI)1097-4660(199806)72:2%3C149::AID-JCTB886%3E3.3.CO;2-3

  76. Cruz PE, Peixoto CC, Moreira JL, Carrondo MJ, 1998. Optimization of the production of virus-like particles in insect cells. Biotechnol Bioeng, 60:408-18. http://dx.doi.org/10.1002/(SICI)1097-0290(19981120)60:4<408::AID-BIT2>3.0.CO;2-Q

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