2009

2009

1. Teixeira AP, Oliveira R, Alves P, Carrondo MJ, 2009. Advances in on-line monitoring and control of mammalian cell cultures: supporting the PAT initiative. Biotechnol Adv, 27:726-32. http://dx.doi.org/10.1016/j.biotechadv.2009.05.003
2. Carmo M, Dias JD, Panet A, Coroadinha AS, Carrondo MJ, Alves PM, Cruz PE, 2009. Thermosensitivity of the reverse transcription process as an inactivation mechanism of lentiviral vectors. Hum Gene Ther, 20(10):1168-76. http://dx.doi.org/10.1089/hum.2009.068
3. Miranda JP, Leite SB, Muller-Vieira U, Rodrigues A, Carrondo MJ, Alves PM, 2009. Toward extended functional hepatocyte in vitro culture. Tissue Eng Part C Methods, 15:1-11. http://dx.doi.org/10.1089/ten.tec.2008.0352
4. Malpique R, Ehrhart F, Katsen-Globa A, Zimmermann H, Alves PM, 2009. Cryopreservation of adherent cells: strategies to improve cell viability and function after thawing. Tissue Eng Part C Methods, 15(3):373-86. http://dx.doi.org/10.1089/ten.tec.2008.0410
5. Mellado MC, Mena JA, Lopes A, Ramirez OT, Carrondo MJ, Palomares LA, Alves PM, 2009. Impact of physicochemical parameters on in vitro assembly and disassembly kinetics of recombinant triple-layered rotavirus-like particles. Biotechnol Bioeng, 104(4):674-86. http://dx.doi.org/10.1002/bit.22430
6. Rodrigues AF, Carmo M, Alves PM, Coroadinha AS, 2009. Retroviral vector production under serum deprivation: the role of lipids. Biotechnol Bioeng, 104(6):1171-81. http://dx.doi.org/10.1002/bit.22499
7. Bernal V, Carinhas N, Yokomizo A, Carrondo MJ, Alves PM, 2009. Cell density effect in the Baculovirus-Insect cells system: a quantitative analysis of energetic metabolism. Biotechnol Bioeng, 104(1):162-80. http://dx.doi.org/10.1002/bit.22364
8. Teixeira AP, Portugal CA, Carinhas N, Dias JM, Crespo JP, Alves PM, Carrondo MJ, Oliveira R, 2009. In situ 2D fluorometry and chemometric monitoring of mammalian cell cultures. Biotechnol Bioeng, 102(4):1098-106. http://dx.doi.org/10.1002/bit.22125
9. Vicente T, Peixoto C, Carrondo MJ, Alves PM, 2009. Purification of recombinant baculoviruses for gene therapy using membrane processes. Gene Ther, 16:766-775. http://dx.doi.org/10.1038/gt.2009.33
10. Carinhas N, Bernal V, Yokomizo AY, Carrondo MJ, Oliveira R, Alves PM, 2009. Baculovirus production for gene therapy: the role of cell density, multiplicity of infection and medium exchange. Appl Microbiol Biotechnol, 81(6):1041-49. http://dx.doi.org/10.1007/s00253-008-1727-4
11. Bannenberg GL, Vieira HL, 2009. Therapeutic applications of the gaseous mediators carbon monoxide and hydrogen sulfide. Expert Opin Ther Pat, 19:663-82. http://dx.doi.org/10.1517/13543770902858824
12. Serra M, Brito C, Costa E, Sousa MF, Alves PM, 2009. Integrating human stem cell expansion and neuronal differentiation in bioreactors. BMC Biotechnol, 9:82. http://dx.doi.org/10.1186/1472-6750-9-82
13. Carmo M, Alves A, Rodrigues AF, Coroadinha AS, Carrondo MJ, Alves PM, Cruz PE, 2009 Stabilization of gammaretroviral and lentiviral vectors: from production to gene transfer. J Gene Med, 11:670-78. http://dx.doi.org/10.1002/jgm.1353
14. Ferreira TB, Perdigão R, Silva AC, Zhang C, Aunins JG, Carrondo MJ, Alves PM, 2009. 293 cell cycle synchronisation in adenovirus vector production. Biotechnol Prog, 25(1):235-43. http://dx.doi.org/10.1002/btpr.64
15. Roldão A, Oliveira R, Carrondo MJ, Alves PM, 2009. Error assessment in recombinant baculovirus titration: evaluation of different Methods. J Virol Methods, 159:69-80. http://dx.doi.org/10.1016/j.jviromet.2009.03.007
16. Serra M, Brito C, Leite SB, Gorjup E, von Briesen H, Carrondo MJ, Alves PM, 2009. Stirred bioreactors for the expansion of adult pancreatic stem cells. Ann Anat, 191:104-15. http://dx.doi.org/10.1016/j.aanat.2008.09.005
17. Vieira HL, Cunha L, Goldmacher VS, Alves PM, 2009. The effect of the cell death suppressor vMIA on the production of a recombinant protein in the adenovirus-293 expression system. Protein Expr Purif, 64(2):179-84. http://dx.doi.org/10.1016/j.pep.2008.11.004
18. Güll I, Alves PM, Gabor F, Wirth M, 2009. Viability of human adenocarcinoma cell line Caco-2: influence of cryoprotectant, freezing rate, and storage temperature. Sci Pharm, 77:133-41. http://dx.doi.org/10.3797/scipharm.0810-07