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2015

  1. Gualda EJ, Pereira H, Vale T, Estrada MF, Brito C, Moreno N. 2015. SPIM-fluid: open source light-sheet based platform for high-throughput imaging. Biomed Opt Express. http://dx.doi.org/10.1364/BOE.6.004447

  2. Nestola P, Peixoto C, Villain L, Alves PM, Carrondo MJT, Mota JPB. 2015. Rational development of two flowthrough purification strategies for adenovirus type 5 and retro virus-like particles, Journal of Chromatography A. http://dx.doi.org/10.1016/j.chroma.2015.11.037

  3. Sousa MFQ, Silva MM, Roldão A, Alves PM, Serra M, Giroux D, Hashimura Y, Wesselschmidt R, Lee B, Carrondo MJT. 2015. Production of oncolytic adenovirus and human mesenchymal stem cells in a single-use, Vertical-Wheel bioreactor system: Impact of bioreactor design on performance of microcarrier-based cell culture processes. Biotechnol Progress. http://dx.doi.org/10.1002/btpr.2158 

  4. Rebelo SP, Costa R, Silva MM, Marcelino P, Brito C, Alves PM. 2015. Three-dimensional co-culture of human hepatocytes and mesenchymal stem cells: improved functionality in long-term bioreactor cultures. J Tissue Eng Regen Med. http://dx.doi.org/10.1002/term.2099

  5. Rebelo SP, Costa R, Estrada M, Shevchenko V, Brito C, Alves PM. 2015. HepaRG microencapsulated spheroids in DMSO‑free culture: novel culturing approaches for enhanced xenobiotic and biosynthetic metabolism. Archives of Toxicology. Published on line 09 August 2014. http://dx.doi.org/10.1007/s00204-014-1320-9

  6. Piersanti S, Burla R, Licursi V, Brito C, La Torre M, Alves PM, Simao D, Mottini C, Salinas S, Negri R, Tagliafico E, Kremer EJ, Saggio I. 2015. Transcriptional Response of Human Neurospheres to Helper-Dependent CAV-2 Vectors Involves the Modulation of DNA Damage Response, Microtubule and Centromere Gene Groups. PLoS One. 10(7):e0133607. http://dx.doi.org/10.1371/journal.pone.0133607  

  7. Rodrigues A F, Guerreiro M R, Formas-Oliveira A S, Fernandes P, Blechert A-K, Genzel Y, Alves P M, Hu W S, Coroadinha AS. 2015. Increased titer and reduced lactate accumulation in recombinant retrovirus production through the down-regulation of HIF1 and PDK. Biotechnology and Bioengineering Jul 1. http://dx.doi.org/10.1002/bit.25691.

  8. Rodrigues AF, Soares HR, Guerreiro MR, Alves PM, Coroadinha AS. 2015. Viral vaccines and their manufacturing cell substrates: New trends and designs in modern vaccinology. Biotechnol J. Sep;10(9):1329-44. http://dx.doi.org/10.1002/biot.201400387.

  9. Silva MM, Rodrigues AF, Correia C, Sousa MQF, Brito C, Coroadinha AS, Serra M, Alves PM. 2015. Robust expansion of human pluripotent stem cells: integration of bioprocess design with transcriptomic and metabolomic characterization. Stem Cells and Translational Medicine, Jul;4(7):731-42 http://dx.doi.org/10.5966/sctm.2014-0270.

  10. Rodrigues AF, Formas-Oliveira AS, Guerreiro M, Tomas H, Alves PM and Coroadinha AS. 2015. Single step cloning-screening method: a new tool for developing and studying high titer viral vector producer cells. Gene Ther, May 4. http://dx.doi.org/10.1038/gt.2015.44

  11. Castro R, Fernandes P, Laske T, Sousa MFQ, Genzel Y, Scharfenberg K, Alves PM, Coroadinha AS. 2015. Production of canine adenovirus type 2 in serum-free suspension cultures of MDCK cells. Appl Microbiol Biotechnol http://dx.doi.org/10.1007/s00253-015-6636-8

  12. Cunha B, Aguiar T, Silva MM, Silva R, Sousa MF, Pineda E, Peixoto C, Carrondo MJ, Serra M, Alves PM. 2015. Exploring continuous and integrated strategies for the up- and downstream processing of human mesenchymal stem cells. Journal of Biotechnology - special issue on integrated continuous biomanufacturing, ahead of print 2015 Mar 4, http://dx.doi.org/10.1016/j.jbiotec.2015.02.023   

  13. Vlaming M, van Duijn E, Dillingh MR, Brands R, Windhorst AD, Hendrikse NH, Bosgra S, Burggraaf J, de Koning MC, Fidder A, Mocking J, Sandman H, de Ligt R, Fabriek BO, Pasman WJ, Seinen W, Alves T, Carrondo M, Peixoto C, Peeters P, Vaes W. 2015 . Microdosing of a carbon-14 labeled protein in healthy volunteers accurately predicts its pharmacokinetics at therapeutic dosages. Clin Pharmacol Ther. Apr 13. http://dx.doi.org/10.1002/cpt.131.

  14. Fernandes P, Almeida AI, Kremer EJ, Alves PM, Coroadinha AS. 2015. Canine helper-dependent vectors production: implications of Cre activity and co-infection on adenovirus propagation. Sci Rep. Mar 16;5:9135. http://dx.doi.org/10.1038/srep09135

  15. Materne EM, Ramme AP, Terrasso AP, Serra M, Alves PM, Brito C, Sakharov DA, Tonevitsky AG, Lauster R, Marx U. 2015. A multi-organ chip co-culture of neurospheres and liver equivalents for long-term substance testing. J Biotechnol. Feb 9. pii: S0168-1656(15)00045-0. http://dx.doi.org/10.1016/j.jbiotec.2015.02.002

  16. Suter-Dick L, Alves PM, Blaauboer BJ, Bremm KD, Brito C, Coecke S, Flick B, Fowler P, Hescheler J, Ingelman-Sundberg M, Jennings P, Kelm JM, Manou I, Mistry P, Moretto A, Roth A, Stedman D, van de Water B, Beilmann M. 2015. Stem Cell-Derived Systems in Toxicology Assessment. Stem Cells Dev. Mar 31. https://doi.org/10.1089/scd.2014.0540

  17. Terrasso AP, Pinto C, Serra M, Filipe A, Almeida S, Ferreira AL, Pedroso P, Brito C, Alves PM. Novel scalable 3D cell based model for in vitro neurotoxicity testing: Combining human differentiated neurospheres with gene expression and functional endpoints. J Biotechnol. 2015 Jan 5. pii: S0168-1656(14)01047-5. http://dx.doi.org/10.1016/j.jbiotec.2014.12.011

  18. Fernandes P, Simão D, Guerreiro MR, Kremer EJ, Coroadinha AS, Alves PM. 2015. Impact of adenovirus life cycle progression on the generation of canine helper-dependent vectors. Gene Ther. Jan;22(1):40-9. http://dx.doi.org/10.1038/gt.2014.92

  19. Sarmento L, Póvoa V, Nascimento R, Real G, Antunes I, Martins L, Moita M, Alves PM, Abecasis M, Moita L, Parkhouse R, Meijerink J and Barata JT. 2015. CHK1 overexpression in T-cell acute lymphoblastic leukemia is essential for proliferation and survival by preventing excessive replication stress. Oncogene. Jun 4;34(23):2978-90. http://dx.doi.org/10.1038/onc.2014.248.

  20. Cunha B, Peixoto C, Silva M, Carrondo MJT, Serra M, Alves PM 2015. Filtration methodologies for the clarification and concentration of human mesenchymal stem cells. Journal of Membrane Science, 478:117-129. http://dx.doi.org/10.1016/j.memsci.2014.12.041

  21. Nestola P, Peixoto C, Silva RJS, Alves PM, Mota JPB, Carrondo MJT 2015. Improved virus purification processes for vaccines and gene therapy. Biotechnology and Bioengineering, 112(5):843-57. http://dx.doi.org/10.1002/bit.25545

  22. Nestola P, Silva RJS, Peixoto C, Alves PM, Carrondo MJT, Mota JPB 2015. Robust design of adenovirus purification by two-column, simulated countercurrent, size-exclusion chromatography. Journal of Biotechnology; in press. http://dx.doi.org/10.1016/j.jbiotec.2015.01.030

  23. Silva AC, Simão D, Sousa MFQ, Küppers C, Lucas T, Cruz P, Carrondo MJ,  Kochanek S, Alves PM 2015 Human Amniocyte-derived Cells for the Production of Adenoviral Vectors. Biotechnology Journal, May;10(5):760-71. http://dx.doi.org/10.1002/biot.201400765

  24. Gomes-Alves P, Serra M, Brito C, Borlado L, López JA, Vázquez J, Carrondo MJ, Bernad A, Alves PM 2015 Exploring analytical proteomics platforms towards the definition of human cardiac stem cells receptome. Proteomics, 15(7):1332-7. http://dx.doi.org/10.1002/pmic.201400318

  25. Simão D, Pinto C, Piersanti S, Weston A, Peddie CJ, Bastos AE, Licursi V, Schwarz SC, Collinson LM, Salinas S, Serra M, Teixeira AP, Saggio I, Lima PA, Kremer EJ, Schiavo G, Brito C, Alves PM. 2015. Modeling human neural functionality in vitro: three-dimensional culture for dopaminergic differentiation. Tissue Eng Part A. 2015 Feb;21(3-4):654-68. http://dx.doi.org/10.1089/ten.TEA.2014.0079

  26. Fernandes, C. S. M., Gonçalves, B., Sousa, M., Martins, D. L., Barroso, T., Pina, A. S., … Roque, A. C. A. 2015. Biobased Monoliths for Adenovirus Purification. ACS Applied Materials & Interfaces, 7(12), 6605–6612. https://doi.org/10.1021/am508907b

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