2018
1. Correia C, Koshkin A, Duarte P, et al. 3D aggregate culture improves metabolic maturation of human pluripotent stem cell derived cardiomyocytes. Biotechnol Bioeng. 2018;115(3):630-644. doi:10.1002/bit.26504.
2. Vidigal J, Fernandes B, Dias MM, et al. RMCE-based insect cell platform to produce membrane proteins captured on HIV-1 Gag virus-like particles. Appl Microbiol Biotechnol. 2018;102(2):655-666. doi:10.1007/s00253-017-8628-3.
3. Sequeira DP, Correia R, Carrondo MJT, Roldão A, Teixeira AP, Alves PM. Combining stable insect cell lines with baculovirus-mediated expression for multi-HA influenza VLP production. Vaccine. 2018;36(22):3112-3123. doi:10.1016/j.vaccine.2017.02.043.
4. Soares HR, Almeida AI, Tomás HA, Alves PM, Coroadinha AS. Flexible pseudotyping of retrovirus using recombinase-mediated cassette exchange. Biotechnol Lett. 2018;40(4):633-639. doi:10.1007/s10529-018-2515-6.
5. Carvalho SB, Moreira AS, Gomes J, et al. A detection and quantification label-free tool to speed up downstream processing of model mucins. PLoS One. 2018;13(1):e0190974. doi:10.1371/journal.pone.0190974.
6. Sebastião MJ, Pereira R, Serra M, Gomes-Alves P, Alves PM. Unveiling Human Cardiac Fibroblast Membrane Proteome. Proteomics. 2018;18(13):e1700446. doi:10.1002/pmic.201700446.
7. Rebelo SP, Pinto C, Martins TR, et al. 3D-3-culture: A tool to unveil macrophage plasticity in the tumour microenvironment. Biomaterials. 2018;163:185-197. doi:10.1016/j.biomaterials.2018.02.030.
8. Schmolka N, Papotto PH, Romero PV, et al. MicroRNA-146a controls functional plasticity in gammadelta T cells by targeting NOD1. Sci Immunol. 2018;3(23):eaao1392. doi: 10.1126/sciimmunol.aao1392
9. B Carvalho S, Fortuna AR, Wolff MW, et al. Purification of influenza virus-like particles using sulfated cellulose membrane adsorbers. J Chem Technol Biotechnol. 2017;93(7). doi:10.1002/jctb.5474.
10. Tomás HA, Rodrigues AF, Carrondo MJT, Coroadinha AS. LentiPro26: Novel stable cell lines for constitutive lentiviral vector production. Sci Rep. 2018;8(5271). doi:10.1038/s41598-018-23593-y.
11. Simão D, Silva MM, Terrasso AP, et al. Recapitulation of Human Neural Microenvironment Signatures in iPSC-Derived NPC 3D Differentiation. Stem cell reports. 2018;11(2):552-564. doi:10.1016/j.stemcr.2018.06.020.
12. Nunes SC, Ramos C, Lopes-Coelho F, et al. Cysteine allows ovarian cancer cells to adapt to hypoxia and to escape from carboplatin cytotoxicity. Sci Rep. 2018;8(1). doi:10.1038/s41598-018-27753-y.
13. Ferreira A, Lamy M, Margarida Rocha M, Silva G, Bandeiras TM, Barbas A. Production and characterization of a novel Delta-like 1 functional unit as a tool for Notch pathway activation and generation of a specific antibody. Protein Expr Purif. 2018;146:8-16. doi:10.1016/j.pep.2018.01.008.
14. Coelho R, Marcos-Silva L, Mendes N, et al. Mucins and Truncated O-Glycans Unveil Phenotypic Discrepancies between Serous Ovarian Cancer Cell Lines and Primary Tumours. Int J Mol Sci. 2018;19(7):E2045. doi:10.3390/ijms19072045.
15. Silva MM, Gomes-Alves P, Rosa S, et al. Full-length human CCBE1 production and purification: leveraging bioprocess development for high quality glycosylation attributes and functionality. J Biotechnol. 2018;285(May):6-14. doi:10.1016/j.jbiotec.2018.08.015.
16. Samaras JJ, Abecasis B, Serra M, Ducci A, Micheletti M. Impact of Hydrodynamics on iPSC-derived Cardiomyocyte Differentiation Processes. J Biotechnol. 2018;287(March):18-27. doi:10.1016/j.jbiotec.2018.07.028.
17. Sebastião MJ, Menta R, Serra M, et al. Human cardiac stem cells inhibit lymphocyte proliferation through paracrine mechanisms that correlate with indoleamine 2,3-dioxygenase induction and activity. Stem Cell Res Ther. 2018;2(290):1-7. doi:10.1186/s13287-018-1010-2
18. Hu D, Linders A, Yamak A, et al. Metabolic Maturation of Human Pluripotent Stem Cell Derived Cardiomyocytes by Inhibition of HIF1α and LDHA Basic Science Research Cell Signaling/Signal Transduction Contractile Function Metabolism Stem Cells. 2018:1066-1079. doi:10.1161/CIRCRESAHA.118.313249.
19. Serra M, Cunha B, Peixoto C, Gomes-Alves P, Alves PM Advancing Manufacture of hMSC Therapies: technological challenges in cell bioprocessing and characterization. Current Opinion in Chemical Engineering. 2018 22: 226-235 doi:10.1016/j.coche.2018.11.003
20. Ana Teresa Serra, Margarida Serra, Ana Carina Silva, et al. Scalable culture strategies for the expansion of patient-derived cancer stem cell lines Stem Cells International. Stem Cells International 2018 doi:10.1155/2019/8347595
21. Bayó-Puxan, N., Terrasso, A.P., Creyssels, S. et al. 2018. Lysosomal and network alterations in human mucopolysaccharidosis type VII iPSC-derived neurons. Sci Rep 8, 16644. https://doi.org/10.1038/s41598-018-34523-3
22. Lemos MAN, Patiño SFS, Bernardino TC, Coroadinha AS, Soares H, Astray RM, Pereira CA, Jorge SAC. 2018. Intracellular Delivery of HCV NS3p gene using vectored particles. J Biotechnol. May 20;274:33-39. https://doi.org/10.1016/j.jbiotec.2018.03.010
23. Moleirinho MG, Rosa S, Carrondo MJT, Silva RJS, Hagner-McWhirter Å, Ahlén G, Lundgren M, Alves PM, Peixoto C. 2018. Clinical-Grade Oncolytic Adenovirus Purification Using Polysorbate 20 as an Alternative for Cell Lysis. Curr Gene Ther. 18(6):366-374. https://doi.org/10.2174/1566523218666181109141257
