2019
1. Abecasis B, Gomes-Alves P, Rosa S, Gouveia PJ, Ferreira LS, Serra M, Alves PM. Unveiling the molecular crosstalk in a human induced pluripotent stem cell derived cardiac model. Biotechnol Bioeng. 2019. doi:10.1002/bit.26929
2. Torán JL, López JA, Gomes-Alves P, Aguilar S, Torroja C, Trevisan-Herraz M, Moscoso I, Sebastião MJ, Serra M, Brito C, Cruz FM, Sepúlveda JC, Abad JL, Galán-Arriola C, Ibanez B, Martínez F, Fernández ME, Fernández-Aviles F, Palacios I, R-Borlado L, Vázquez J, Alves PM, Bernad A. Definition of a cell surface signature for human cardiac progenitor cells after comprehensive comparative transcriptomic and proteomic characterization. Sci Rep. 2019. Mar 15;9(1):4647. doi: 10.1038/s41598-019-39571-x
3. Soares HR, Castro R, Tomás HA, Carrondo MJT, Alves PM, Coroadinha AS. Pseudotyping retrovirus like particles vaccine candidates with Hepatitis C virus envelope protein E2 requires the cellular expression of CD81. AMB Express. 2019. Feb 7;9(1):22. doi: 10.1186/s13568-019-0741-5.
4. Sebastião MJ, Serra M, Pereira R, Palacios I, Gomes-Alves P, Alves PM. Human cardiac progenitor cell activation and regeneration mechanisms: exploring a novel myocardial ischemia/reperfusion in vitro model. Stem Cell Res Ther. 2019 Mar 7;10(1):77. doi: 10.1186/s13287-019-1174-4.
5. Rego D, Redondo L, Casaleiro S, Sousa AP, Abreu S, Serra M, Espirito Santo V. Application of pulsed electric fields for the valorization of platelets with no therapeutic value for transfusion medicine. Technology 7:40-45. doi: 10.1142/S2339547819500031
6. Charlton Hume HK, Vidigal J, Carrondo MJT, Middelberg APJ, Roldão A, Lua LHL. Synthetic biology for bioengineering virus-like particle vaccines. Biotechnol Bioeng. 2019 Apr;116(4):919-935. doi: 10.1002/bit.26890
7. Guerreiro MR, Freitas DF, Alves PM, Coroadinha AS. Detection and Quantification of Label-Free Infectious Adenovirus Using a Switch-On Cell-Based Fluorescent Biosensor. ACS Sens. 2019 Jun 28;4(6):1654-1661. doi: 10.1021/acssensors.9b00489
8. Carvalho SB, Silva JS, Moreira AS, Cunha B, Clemente JJ, Alves PM, Carrondo MJT, Xenopoulos A, Peixoto C. Efficient filtration strategies for the clarification of influenza virus-like particles derived from insect cells. Separation and Purification Technology. 2019; 218: 81-88. doi: 10.1016/j.seppur.2019.02.040
9. Pinto C, Silva G, Ribeiro AS, Oliveira M, Garrido M, Bandeira VS, Nascimento A, Coroadinha AS, Peixoto C, Barbas A, Paredes J, Brito C, Alves PM. Evaluation of AAV-mediated delivery of shRNA to target basal-like breast cancer genetic vulnerabilities. J Biotechnol. 2019 Jul 20;300:70-77. doi: 10.1016/j.jbiotec.2019.05.016.
10. Pais DAM, Portela RMC, Carrondo MJT, Isidro IA, Alves PM. Enabling PAT in insect cell bioprocesses: In situ monitoring of recombinant adeno-associated virus production by fluorescence spectroscopy. Biotechnol Bioeng. 2019 Jul 17 doi: 10.1002/bit.27117
11. Carvalho SB, Silva RJS, Moleirinho MG, Cunha B, Moreira AS, Xenopoulos A, Alves PM, Carrondo MJT, Peixoto C. Membrane‐Based Approach for the Downstream Processing of Influenza Virus‐Like Particles. Biotechnol J. 2019. 14(8): e1800570. doi: 10.1002/biot.201800570
12. Soares HR, Ferreira-Fernandes M, Marchel M, Alves PM, Coroadinha AS. Enhancing Hepatitis C Virus pseudoparticles infectivity through p7NS2 cellular expression. J Virol Methods. 2019. 11:113714. doi: 10.1016/j.jviromet.2019.113714
13. Sousa M, Fenge C, Rupprecht J, Tappe A, Greller G, Alves P, Carrondo M, Roldão A. Process intensification for Peste des Petites Ruminants Virus vaccine production. Vaccine. 2019. doi: 10.1016/j.vaccine.2019.07.009
14. Moleirinho MG, Silva RJS, Carrondo MJT, Alves PM, Peixoto C. Exosome-based therapeutics: Purification using semi-continuous multi-column chromatography. Separation and Purification Technology. 2019; 224:515-523. doi: 10.1016/j.seppur.2019.04.060
15. Sebastião MJ, Gomes-Alves P, Reis I, Sanchez B, Palacios I, Serra M, Alves PM. Bioreactor-based 3D human myocardial ischemia/reperfusion in vitro model: a novel tool to unveil key paracrine factors upon AMI. Translational Research. 2019. doi: 10.1016/j.trsl.2019.09.001
16. Arez F, Rebelo SP, Fontinha D, Simão D, Martins TR, Machado M, Fischli C, Oeuvray C, Badolo L, Carrondo MJT, Rottmann M, Spangenberg T, Brito C, Greco B, Prudêncio M, Alves PM. Flexible 3D Cell-Based Platforms for the Discovery and Profiling of Novel Drugs Targeting Plasmodium Hepatic Infection. ACS Infectious Diseases. 2019. doi: 10.1021/acsinfecdis.9b00144
17. Fernandes B, Vidigal J, Correia R, Carrondo MJT, Alves PM, Teixeira AP, Roldão A. Adaptive laboratory evolution of stable insect cell lines for improved HIV-Gag VLPs production. J Biotechnol. 2019. doi: 10.1016/j.jbiotec.2019.10.004
18. Tomás HA, Mestre DA, Rodrigues AF, Guerreiro MR, Carrondo MJT, Coroadinha AS. Improved GaLV-TR Glycoproteins to Pseudotype Lentiviral Vectors: Impact of Viral Protease Activity in the Production of LV Pseudotypes. Mol Ther Methods Clin Dev. 2019. 15:1-8. doi:10.1016/j.omtm.2019.08.001
19. Suárez-Patiño SF, Bernardino TC, Núñez EGF, Astray RM, Pereira CA, Soares HR, Coroadinha AS, Jorge SAC. Semliki Forest Virus replicon particles production in serum-free medium BHK-21 cell cultures and their use to express different proteins. Cytotechnology. 2019. 71(5): 949-962. doi:10.1007/s10616-019-00337-y
20. Marchel M, Soares HR, Vormittag P, Hubbuch J, Coroadinha AS, Marrucho IM. High-throughput screening of aqueous biphasic systems with ionic liquids as additives for extraction and purification of enveloped virus-like particles. Engineering Reports. 2019. 1(1): e12030. doi:10.1002/eng2.12030
21. Moleirinho MG, Silva RJS, Alves PM, Carrondo MJT, Peixoto C. Current challenges in biotherapeutic particles manufacturing. Expert Opinion on Biological Therapy. 2019. doi:10.1080/14712598.2020.1693541
22. Sales-Dias J, Silva G, Lamy M, Ferreira A, Barbas A. The Notch ligand DLL1 exerts carcinogenic features in human breast cancer cells. PLoS One. 2019. 14(5): e0217002. doi: 10.1371/journal.pone.0217002
