2020

1.         Raposo CD, Costa R, Petrova KT, Brito C, Scotti MT, Cardoso MM. Development of Novel Galactosylated PLGA Nanoparticles for Hepatocyte Targeting Using Molecular Modelling. Polymers. 2020. 12(1): 94. doi:10.3390/polym12010094

2.        Moreira AS, Silva AC, Sousa MFQ, Hagner-McWhirterc A, Ahlénc G, Lundgren M, Coroadinha AS, Alves PM, Peixoto C, Carrondo MJT. Establishing Suspension Cell Cultures for Improved Manufacturing of Oncolytic Adenovirus. Biotechnol J. 2020. 16:e1900411. doi:10.1002/biot.201900411

3.        Silva RJS, Moleirinho MG, Moreira AS, Xenopoulos A, Alves PM, Carrondo MJT, Peixoto C. A Flow-Through Chromatographic Strategy for Hepatitis C Virus-Like Particles Purification. Processes. 2020. 8(1): 85. doi:10.3390/pr8010085

4.        Lopes-Coelho F, Silva F, Gouveia-Fernandes S, Martins C, Lopes N, Domingues G, Brito C, Almeida AM, Pereira SA, Serpa J. Monocytes as endothelial percursor cells (EPCs), another brick in the wall to disentangle tumor angiogenesis. Cells. 2020. 9(1): 107. doi:10.3390/cells9010107

5.        Moleirinho MG, Fernandes RP, Carvalho SB, Bezemer S, Detmers F, Hermans P, Silva RJS, Alves PM, Carrondo MJT, Peixoto C. Baculovirus affinity removal in viral-based bioprocesses. Separation and Purification Technology. 2020. 241: 116693. doi:10.1016/j.seppur.2020.116693

6.        Vasconcelos e Sá J, Simão D, Terrasso AP, Silva MM, Brito C, Isidro IA, Alves PM, Carrondo MJT. Unveiling dynamic metabolic signatures in human induced pluripotent and neural stem cells. PLOS Computational Biology. 2020. 16(4): e1007780. doi:10.1371/journal.pcbi.1007780

7.        Pais DA, Galrão PR, Kryzhanska A, Barbau J, Isidro IA, Alves PM. Holographic Imaging of Insect Cell Cultures: Online Non-Invasive Monitoring of Adeno-Associated Virus Production and Cell Concentration. Processes. 2020. 8(4): 487. doi:10.3390/pr8040487

8.        Formas-Oliveira AS, Basílio JS, Rodrigues AF, Coroadinha AS. Overexpression of ER Protein Processing and Apoptosis Regulator Genes in Human Embryonic Kidney 293 Cells Improves Gene Therapy Vectors Production. Biotechnology Journal. 2020. 1900562. doi:10.1002/biot.201900562

9.        Ferreira MV, Cabral ET, Coroadinha AS. Progress and Perspectives in the Development of Lentiviral Vector Producer Cells. Biotechnology Journal. 2020. 2000017. doi:10.1002/biot.202000017

10.        Sousa BB, Sousa MFQ, Marques MC, Seixas JD, Brito JA, Matias PM, Bernardes GJL, Roldão A. A scalable insect cell-based production process of the human recombinant BMX for in-vitro covalent ligand high-throughput screening. Bioprocess and Biosystems Engineering. 2020. 43(9). doi:10.1007/s00449-020-02421-6

11.        Cartaxo AS, Estrada MF, Domenici G, Roque R, Silva F, Gualda EJ, Loza-Alvarez P, Sflomos G, Brisken C, Alves PM, André S, Brito C. A novel culture method that sustains ERα signaling in human breast cancer tissue microstructures. Journal of Experimental & Clinical Cancer Research. 2020. 39:161. doi:10.1186/s13046-020-01653-4

12.        Rodrigues AF, Fernandes P, Laske T, Castro R, Alves PM, Genzel Y, Coroadinha AS. Cell Bank Origin of MDCK Parental Cells Shapes Adaptation to Serum-Free Suspension Culture and Canine Adenoviral Vector Production. International Journal of Molecular Sciences. 2020. 21(17):6111. doi:10.3390/ijms21176111

13.        Moleirinho MG, Feast S, Moreira AS, Silva RJS, Alves PM, Carrondo MJT, Huber T, Fee C, Peixoto C. 3D-printed ordered bed structures for chromatographic purification of enveloped and non-enveloped viral particles. Separation and Purification Technology. 2020. 254:117681. doi:10.1016/j.seppur.2020.117681

14.        Abecasis B, Canhão PGM, Almeida HV, Calmeiro T, Fortunato E, Gomes-Alves P, Serra M, Alves PM. Towards a microencapsulated 3D hiPSC-derived in vitro cardiac microtissue for recapitulation of human heart microenvironment features. Frontiers in Bioengineering and Biotechnology. 2020. 8:580744. doi:10.3389/fbioe.2020.580744

15.        Cartaxo AL, Almeida J, Gualda EJ, Marsal M, Loza-Alvarez P, Brito C, Isidro IA. A computational diffusion model to study antibody transport within reconstructed tumor microenvironments. BMC Bioinformatics. 2020. 21:529. doi: 10.1186/s12859-020-03854-2

16.       Pais DAM, Brown C, Neuman A, Mathur K, Isidro IA, Alves PM, Slade PG. Dielectric spectroscopy to improve the production of rAAV used in Gene Therapy. Processes. 2020. 8(11):1456. doi:10.3390/pr8111456

17.       Abreu S, Silva F, Mendes R, Mendes TF, Teixeira M, Santo VE, Boghaert E, Félix A, Brito C. Patient-derived ovarian cancer explants: preserved viability and histopathological features in long-term agitation-based cultures. Scientific Reports. 2020. 10:19462. doi: 10.1038/s41598-020-76291-z

18.       Moreira AS, Cavaco D, Faria T, Alves PM, Carrondo MJT, Peixoto C. Advances in  Lentivirus Purification. Biotechnology Journal. 2020. doi:10.1002/biot.202000019

19. Guerreiro MR, Fernandes AR, Coroadinha AS. Evaluation of Structurally Distorted Split GFP Fluorescent Sensors for Cell-Based Detection of Viral Proteolytic Activity. Sensors. 2020. 21(1), 24. doi:10.3390/s21010024

20. Correia R, Fernandes B, Alves PM, Carrondo MJT, Roldao A. Improving Influenza HA-Vlps Production in Insect High Five Cells via Adaptive Laboratory Evolution. Vaccines. 2020. 8(4), 589. doi:10.3390/vaccines8040589

21. Thomas D, Sagar S, Liu X, et al. Isoforms of MUC16 activate oncogenic signaling through EGF receptors to enhance the progression of pancreatic cancer. Molecular Therapy. 2020. doi:10.1016/j.ymthe.2020.12.029

22.  Lobo SA, Bączyk P, Wyss B, Widmer JC, Jesus LP, Gomes J, Batista AP, Hartmann S, Wassmann P. Stability liabilities of biotherapeutic proteins: Early assessment as mitigation strategy. Journal of Pharmaceutical and Biomedical Analysis. 2020. https://doi.org/10.1016/j.jpba.2020.113650