Melanoma skin in vitro model

Cutaneous melanoma is the most aggressive form of skin cancer and one of the leading causes of cancer-related mortality due to its high metastatic ability.  The knowledge of tumor biology, molecular alterations and the interplay between the immune system in melanoma has led to the development of new therapeutic targets and consequently, the improvement of patient care.

Over the last decades, strong investment has been directed to the development of in vitro human skin models that closely mimic in vivo healthy and diseased skin. One of the central issues with cancer therapy is the low reproducibility between results observed in vitro and in clinical trials. 3D cultures may have a significant impact on the therapy of patients with melanoma and reduce animal testing.

In this context, this study aims to produce an innovative melanoma model with cells involved in tumor immune response (peripheral blood mononuclear cells, PBMCs).

The main goal of this project is to develop an humanized 3D melanoma model that includes dermis and epidermis with melanocytes and melanoma cells.