Publications

Since 2015

Sandau US, Magaña SM, Costa J, Nolan JP, Ikezu T, Vella LJ, Jackson HK, Moreira LR, Palacio PL, Hill AF, Quinn JF, Van Keuren-Jensen KR, McFarland TJ, Palade J, Sribnick EA, Su H, Vekrellis K, Coyle B, Yang Y, Falcón-Perez JM, Nieuwland R, Saugstad JA; International Society for Extracellular Vesicles Cerebrospinal Fluid Task Force (2024) Recommendations for Reproducibility of Cerebrospinal Fluid Extracellular Vesicle Studies. J. Extracel. Ves. 13:12397. doi: 10.1002/jev2.12397. 

Vicente J, Guerreiro AC, Felgueiras B, Chapla DG, Moremen K, Costa J (2023) Glycosyltransferase 8 domain-containing protein 1 (GLT8D1) is a UDP-dependent galactosyltransferase. Sci. Rep. 13:21684. doi: 10.1038/s41598-023-48605-480.

Costa J, Hayes C, Lisacek F (2023) Protein glycosylation and glycoinformatics for novel biomarker discovery in neurodegenerative diseases. Aging Res. Rev. 89, 101991. https://doi.org/10.1016/j.arr.2023.101991.

Fernandes B, Castro R, Bhoelan F, Bemelman D, Gomes RA, Costa J, Gomes-Alves P, Stegmann T, Amacker M, Alves PM, Fleury S, Roldão A (2022) Insect Cells for High-Yield Production of SARS-CoV-2 Spike Protein: Building a Virosome-Based COVID-19 Vaccine Candidate. Pharmaceutics 14, 854. doi: 10.3390/pharmaceutics14040854. 

Neto A, Pinheiro Torres R, Ramiro S, Sardoo A, Rodrigues-Manica S, Lagoas-Gomes J, Domingues L, Lage Crespo C, Teixeira D, Sepriano A, Masi AT, Nair K, Gomes-Alves P, Costa J, Branco JC, Pimentel-Santos F (2022) Muscle dysfunction in axial spondylarthritis: the MyoSpA study. Clin. Experim. Rheumatol. 40, 267-273. doi: 10.55563/clinexprheumatol/9ljng7

Pimentel-Santos F, Manica SR, Masi AT, Lagoas-Gomes J, Santos MB, Ramiro S, Sepriano A, Nair K, Gomes-Alves P, Costa J, Branco JC (2021) Lumbar myofascial physical properties in healthy adults: myotonometry vs. shear wave elastography measurements. Acta Reumatol. Port. 46, 110-119. PMID: 34226434

Costa J, Gromicho M, Pronto-Laborinho A, Almeida C, Gomes RA, Guerreiro ACL, Oliva A, Pinto S, de Carvalho M (2021) Cerebrospinal fluid chitinases as biomarkers for amyotrophic lateral sclerosis. Diagnostics 11, 1210. doi: 10.3390/diagnostics11071210

Castro R, Nobre LS, Eleutério RP, Thomaz M, Pires A, Monteiro SM, Mendes S, Gomes RA, Clemente JJ, Sousa MFQ, Pinto F, Silva AC, Freitas MC, Lemos AR, Akpogheneta O, Kosack L, Bergman M-L, Duarte N, Matoso P, Costa J, Bandeiras TM, Gomes-Alves P, Gonçalves CP, Demengeot J, Alves PM (2021) Production of high-quality SARS-CoV-2 antigens: impact of bioprocess and storage on glycosylation, biophysical attributes, and ELISA serologic tests performance. Biotech. Bioengineer. 118, 2202-2219. doi: 10.1002/bit.27725

Costa J, Pronto-Laborinho A, Pinto S, Gromicho M, Bonucci S, Tranfield E, Correia C, Alexandre BM, de Carvalho M (2020) Investigating LGALS3BP/90K glycoprotein in the cerebrospinal fluid of patients with neurological diseases. Sci. Rep. 10, 5649. doi: 10.1038/s41598-020-62592-w.

Costa J, Streich L, Pinto S, Pronto-Laborinho A, Nimtz M, Conradt HS, de Carvalho M (2019) Exploring Cerebrospinal Fluid IgG N-Glycosylation as Potential Biomarker for Amyotrophic Lateral Sclerosis. Molecular Neurobiol. 56, 5729–5739. doi: 10.1007/s12035-019-1482-9.

Costa J, Gatermann M, Nimtz M, Kandzia S, Glatzel M, Conradt HS (2018) N-Glycosylation of extracellular vesicles from HEK-293 and glioma cell lines. Anal Chem. 90, 7871-7879. doi: 10.1021/acs.analchem.7b05455.

Carvalho SB, Moreira AS, Gomes J, Carrondo MJT, Thorntond DJ, Alves PM, Costa J, Peixoto C (2018) A detection and quantification label-free tool to speed up downstream processing of mucins. PLOS One. 13, e0190974. doi: 10.1371/journal.pone.0190974.

Costa J (2017) Glycoconjugates from extracellular vesicles: Structures, functions and emerging potential as cancer biomarkers. BBA Reviews on Cancer. 1868, 157-166. doi: 10.1016/j.bbcan.2017.03.007.

Gonçalves M, de Carvalho M, Peixoto C, Alves P, Barreto C, Oliva A, Pinto S, Laborinho-Pronto A, Gromicho G, Costa J (2017) Phosphoneurofilament heavy chain and vascular endothelial growth factor as cerebrospinal fluid biomarkers for Amyotrophic Lateral Sclerosis. Amyotrophic Lateral Sclerosis and Frontotemporal Degeneration 18, 134-136. doi: 10.1080/21678421.2016.1212894.

Oeckl P, Jardel C, Salachas F, Lamari F, Andersen PM, Bowser R, de Carvalho M, Costa J, van Damme P, Gray E, Grosskreutz J, Hernández-Barral M, Herukka SK, Huss A, Jeromin A, Kirby J, Kuzma-Kozakiewicz M, Amador MM, Mora JS, Morelli C, Muckova P, Petri S, Poesen K, Rhode H, Rikardssond AK, Robberecht W, Mahillo AIR, Shaw P, Silani V, Steinacker P, Turner MR, Tüzün E, Yetimler B, Ludolph AC, Otto M (2016) Multicenter validation of CSF neurofilaments as diagnostic biomarkers for ALS. Amyotrophic Lateral Sclerosis and Frontotemporal Degeneration 17, 404-413. doi: 10.3109/21678421.2016.1167913.

Costa J, de Carvalho M (2016)  Emerging molecular biomarker targets for amyotrophic lateral sclerosis. Clin. Chim. Acta. 455, 7-14. doi: 10.1016/j.cca.2016.01.011.

Alves M, Lamego J, Bandeiras T, Castro R, Tomás H, Coroadinha AS, Costa J, Simplício AL (2016) Human carboxylesterase 2: Studies on the role of glycosylation for enzymatic activity. Biochemistry and Biophysics Reports.  5, 105-110. doi: 10.1016/j.bbrep.2015.11.018. 

Gonçalves M, Tillack L, de Carvalho M, Pinto S, Conradt HS, Costa J (2015) Phosphoneurofilament heavy chain and N-glycomics from the cerebrospinal fluid in Amyotrophic Lateral Sclerosis. Clin. Chim. Acta 438, 342-349. doi: 10.1016/j.cca.2014.09.011.

Gomes J, Gomes-Alves P, Carvalho SB, Peixoto C, Alves PM, Altevogt P, Costa J (2015) Extracellular Vesicles from Ovarian Carcinoma Cells Display Specific Glycosignatures. Biomolecules 5, 1741-61. doi: 10.3390/biom5031741. 

From 1998 to 2014

Nimtz M, Grabenhorst E, Gambert U, Costa J, Wray V, Morr M, Thiem J, Conradt HS (1998) In vitro α1-3 or α1-4 fucosylation of type I and type II oligosaccharides with secreted forms of recombinant human fucosyltransferases III and VI. Glycoconjug. J. 15, 873-883. 

Glathe S, Kervinen J, Nimtz M, Li GH, Tobin GJ, Copeland TD, Ashford DA, Wlodawer A, Costa J (1998) Transport and activation of the vacuolar aspartic proteinase phytepsin in barley (Hordeum vulgare L.). J. Biol. Chem. 273, 31230-31236. 

Grabenhorst E, Nimtz M, Costa J, Conradt HS (1998) In vivo specificity of human α1,3/4-fucosyltransferases III-VII in the biosynthesis of Lewis X and sialyl-Lewis X motifs on complex-type N-glycans: coexpression studies from BHK-21 cells together with human β-trace protein. J. Biol. Chem. 273, 30985-30994. 

Costa J, Costa MT, Grabenhorst E, Nimtz M, Conradt HS (1998) Construction of recombinant BHK cell lines expressing wild-type and mutants of human α1,3/4-fucosyltransferase. Carbohyd. Polym. 37, 287-290. 

Kervinen J, Tobin GJ, Costa J, Waugh DS, Wlodawer A, Zdanov A (1999) Crystal structure of plant aspartic proteinase prophytepsin: inactivation and vacuolar targeting. EMBO J. 18, 3947-3955.

Frazão C, Bento I, Costa J, Soares CM, Veríssimo P, Faro C, Pires E, Cooper J, Carrondo MA (1999) Crystal structure of cardosin A, a glycosylated and Arg-Gly-Asp containing aspartic proteinase from the flowers of Cynara cardunculus L. J. Biol. Chem. 274, 27694-27701. 

Sousa VL, Costa MT, Palma AS, Enguita F, Costa J (2001) Localization, purification and specificity of the full-length membrane bound form of human recombinant α1,3/4-fucosyltransferase from BHK-21B cells. Biochem. J. 357, 803-810. 

Palma AS, Verde CV, Pires AS, Fevereiro PS, Costa J (2001) A novel plant α4-fucosyltransferase (Vaccinium myrtillus L.) synthesises the Lewisa adhesion determinant. FEBS Let. 499, 235-238. 

Morais VA, Serpa J, Palma AS, Costa T, Maranga L, Costa J (2001) Expression and characterization of recombinant human α-3/4-fucosyltransferase III from Spodoptera frugiperda (Sf9) and Trichoplusia ni (Tn) cells using the baculovirus expression system. Biochem. J. 353, 719-725. 

Teixeira A, David L, Reis CA, Costa J, Sobrinho-Simões M (2002) Expression of mucins (MUC1, MUC2, MUC5AC and MUC6) and type 1 Lewis antigens in cases with and without Helicobacter pylori colonization in metaplastic glands of the stomach. J. Pathol. 197, 37-43. 

Morais V, Costa MT, Costa J (2003) N-glycosylation of recombinant human fucosyltransferase III is required for its in vivo folding in mammalian and insect cells. Biochim. Biophys. Acta (General Subjects) 1619, 133-138. 

Sousa VL, Brito C, Costa T, Lanoix J, Nilsson T, Costa J (2003) Importance of Cys, Gln, and Tyr from the transmembrane domain of human α3/4-fucosyltransferase III for its localization and sorting in the Golgi of baby hamster kidney cells. J. Biol. Chem. 278, 7624-7629.

Morais VA, Crystal AS, Pijak DS, Carlin D, Costa J, Lee VM, Doms RW (2003) The transmembrane domain region of nicastrin mediates direct interactions with APH-1 and the gamma-secretase complex. J. Biol. Chem. 278, 43284-43291. 

Morais, V.A., Costa, J. (2003) Stable expression of recombinant α3/4 fucosyltransferase III in Spodoptera frugiperda Sf9 cells. J. Biotechnol. 106, 69-75. 

 Palma AS, Morais VA, Coelho AV, Costa J (2004) Effect of the manganese ion on human α3/4 fucosyltransferase III activity. Biometals 17, 35-43. 

Sousa VL, Brito C, Costa J (2004) Deletion of the cytoplasmic domain of human α3/4 fucosyltransferase III causes the shift of the enzyme to early Golgi compartments. Biochim. Biophys. Acta (General Subjects) 1675, 95-104. 

Marcos NT, Pinho S, Grandela C, Cruz A, Samyn-Petit B, Harduin-Lepers A, Almeida R, Silva F, Morais V, Costa J, Kihlberg J, Clausen H, Reis CA (2004) Role of the human ST6GalNAc-I and ST6GalNAc-II in the synthesis of the cancer-associated sialyl-Tn antigen. Can. Res. 64, 7050-7057. 

Palma A, de Carvalho M, Barata N, Evangelista T, Chicau P, Regalla M, Costa J (2005) Biochemical characterization of plasma in amyotrophic lateral sclerosis: amino acid and protein composition. Amyotrophic Lateral Sclerosis 6, 104-110.

Henriques ST, Costa J, Castanho MARB (2005) Translocation of β-galactosidase mediated by the cell-penetrating peptide pep-1 into lipid vesicles and human HeLa cells is driven by membrane electrostatic potential. Biochemistry 44, 10189-10198.

Henriques ST, Costa J, Castanho MARB (2005) Re-evaluating the role of strongly charged sequences in amphipathic cell-penetrating peptides. A fluorescence study using Pep-1. FEBS Let. 579, 4498-4502.

Palma A, Feizi T, Zhang Y, Stoll M S, Lawson AM, Diaz-Rodriguez E, Campanero-Rhodes MA, Costa J, Gordon S, Brown GD, Chai W (2006) Ligands for the b-glucan receptor, Dectin-1, assigned using "designer" microarrays of oligosaccharide probes (neoglycolipids) generated from glucan polysaccharides. J. Biol. Chem. 281, 5771-5779.

Escrevente C, Machado E, Brito C, Reis CA, Stoeck A, Runz S, Marmé A, Altevogt P, Costa J (2006) Different expression levels of α3/4 fucosyltransferases and Lewis determinants in ovarian carcinoma tissues and cell lines. Int. J. Oncol. 29, 557-566. 

Morais VA, Brito C, Pijak DS, Crystal AS, Fortna RR, Li T, Wong PC, Doms RW, Costa J (2006) N-Glycosylation of human nicastrin is required for full g-secretase complex activity and for interaction with calnexin and ERGIC-53. Biochim. Biophys. Acta - Molecular Basis of Disease 1762, 802-810. 

Gouveia R, Morais VA, Peixoto C, Sousa M, Regalla M, Alves P, Costa J (2007) Production and purification of functional truncated soluble forms of human recombinant L1 cell adhesion glycoprotein from Spodoptera frugiperda Sf9 cells. Prot. Exp. Purif. 52, 182-193.

Brito C, Escrevente C, Reis C, Lee VM, Trojanowski JQ, Costa J (2007) Increased levels of fucosyltransferase IX and carbohydrate Lewisx adhesion determinant in human NT2N neurons. J. Neurosci. Res. 85, 1260-1270. 

Brito C, Gouveia R, Costa J (2007) Stable expression of an active soluble recombinant form of human fucosyltransferase IX in Spodoptera frugiperda Sf9 cells. Biotechnol. Let. 29, 1623-1630.

Gomes C, Keller S, Altevogt P, Costa J (2007) Evidence for secretion of Cu, Zn superoxide dismutase via exosomes from a cell model of amyotrophic lateral sclerosis. Neurosci. Let. 428, 43-46. doi: 10.1016/j.neulet.2007.09.024. 

Serra M, Leite SB, Brito C, Costa J, Carrondo MJ, Alves PM (2007) Novel culture strategy for human stem cell proliferation and neuronal differentiation. J. Neurosci. Res. 85, 3557-3566.

Gomes C, Palma AS, Almeida R, Regalla M, McCluskey LF, Trojanowski JQ, Costa J (2008) Establishment of a cell model of ALS disease: Golgi apparatus disruption independent from apoptosis. Biotechnol. Let. 30, 603-610. 

Morais VA, Leight S, Pijak DS, Lee VMY, Costa J (2008) Cellular localization of Nicastrin affects amyloid beta species production FEBS Let. 582, 427-433. doi: 10.1016/j.febslet.2008.01.003. 

Brito C, Kandzia S, Graça T, Conradt HS, Costa J (2008) Human fucosyltransferase IX efficiently fucosylates N-linked asialoglycoproteins with the synthesis of the adhesion Lewisx determinant. Biochimie 90, 1279-1290. doi: 10.1016/j.biochi.2008.03.002. Front cover.

Escrevente C, Morais VA, Keller S, Soares CM, Altevogt P, Costa J (2008) Functional role of N-glycosylation from ADAM10 in processing, activity, stability and localization of the enzyme. Biochimica Biophysica Acta - General subjects 1780, 905-913. doi: 10.1016/j.bbagen.2008.03.004. 

Gouveia RM, Gomes CM, Sousa M, Alves PM, Costa J (2008) Kinetic analysis of L1 homophilic interaction. Role of the first four immunoglobulin domains and implications on binding mechanism. J. Biol. Chem. 283, 28038-28047. doi: 10.1074/jbc.M804991200.

Palma AS, de Carvalho M, Grammel N, Pinto S, Barata N, Conradt HS, Costa J (2008) Proteomic analysis of plasma from Portuguese patients with Familial Amyotrophic Lateral Sclerosis. Amyotrophic Lateral Sclerosis 9, 339-349. doi: 10.1080/17482960801934239. 

Brito C, Danglot L, Galli T, Costa J (2009) Subcellular localization of the carbohydrate Lewisx adhesion structure in hippocampus cell cultures. Brain Res. 1287, 39-46. doi: 10.1016/j.brainres.2009.06.075.

Carvalho AS, Harduin-Lepers A, Magalhães A, Machado E, Mendes N, Costa LT, Matthiesen R, Almeida R, Costa J, Reis CA (2010) Differential expression of alpha2,3-sialyltransferases and alpha1,3/4-fucosyltransferases regulates the levels of sialyl Lewis a and sialyl Lewis x in gastrointestinal carcinoma cells. Int. J. Biochem. Cell Biol. 42, 80-89. doi: 10.1016/j.biocel.2009.09.010.

Gouveia R, Kandzia S, Conradt HS, Costa J (2010) N-glycosylation of human cell adhesion molecule L1 expressed in insect cells using the stable expression system. Effect of dimethyl sulfoxide. J. Biotechnol. 145, 130-138. doi: 10.1016/j.jbiotec.2009.10.018.

Wolterink S, Moldenhauer G, Fogel M, Kiefel H, Pfeifer M, Lüttgau S, Gouveia R, Costa J, Endell J, Moebius U, Altevogt P (2010) Therapeutic antibodies to human L1CAM: functional characterization and application in a mouse model for ovarian carcinoma. Cancer Res. 70, 2504-2515. doi: 10.1158/0008-5472.CAN-09-3730.

Kristiansen G, Machado E, Bretz N, Rupp C, Winzer KJ, König AK, Moldenhauer G, Marmé F, Costa J, Altevogt P (2010) Molecular and clinical dissection of CD24 antibody specificity by a comprehensive comparative analysis. Lab Invest. 90, 1102-1116. doi: 10.1038/labinvest.2010.70.

Gomes C, Escrevente C, Costa J (2010) Mutant superoxide dismutase 1 overexpression in NSC-34 cells: Effect of trehalose on aggregation, TDP-43 localization and levels of co-expressed glycoproteins. Neurosci. Lett. 475, 145–149. doi: 10.1016/j.neulet.2010.03.065.

Costa J, Gomes C, de Carvalho M (2010) Diagnosis, Pathogenesis and Therapeutic Targets in Amyotrophic Lateral Sclerosis (Review article). CNS Neurol Disord Drug Targets 9, 764-78.

Machado E, Kandzia S, Carilho R, Altevogt P, Conradt HS, Costa J (2011) N-Glycosylation of total cellular glycoproteins from the human ovarian carcinoma SKOV3 cell line and of recombinantly expressed human erythropoietin. Glycobiology 21, 376-86. doi: 10.1093/glycob/cwq170. 

Escrevente C, Keller S, Altevogt P, Costa J (2011) Interaction and uptake of exosomes by ovarian cancer cells. BMC Can.11, 108. doi: 10.1186/1471-2407-11-108.

Jorge CD, Ventura R, Maycock C, Outeiro TF, Santos H, Costa J (2011)  Assessment of the efficacy of solutes from extremophiles on protein aggregation in cell models of Huntington´s and Parkinson´s diseases. Neurochem. Res. 36, 1005-1011. doi: 10.1007/s11064-011-0440-3. 

Carilho R, de Carvalho M, Kuehl U, Pinto S, Pinto A, Kromminga A, Costa J (2011) Erythropoietin and Amyotrophic Lateral Sclerosis: plasma level determination. Amyotrophic Lateral Sclerosis 12, 439-443. doi: 10.3109/17482968.2011.584881.

Otto M, Bowser R, Turner M, Berry J, Brettschneider J, Connor J, Costa J, Cudkowicz  M, Glass J, Jahn O, Lehnert S, Malaspina A, Parnetti L, Petzold A, Shaw P, Sherman A, Steinacker P, Süßmuth S, Teunissen C, Tumani H, Wuolikainen A, Ludolph A (2012) Roadmap and standard operating procedures for biobanking and discovery of neurochemical markers in ALS. Amyotrophic Lateral Sclerosis 13, 1-10. doi: 10.3109/17482968.2011.627589.

Gouveia R, Schaffer L, Papp S, Kandzia S, Head SR, Kleene R, Schachner M, Conradt HS, Costa J (2012) Expression of glycogenes in differentiating human NT2N neurons. Downregulation of fucosyltransferase 9 leads to decreased Lewisx levels and impaired neurite outgrowth. Biochimica Biophysica Acta - General subjects 1820, 2007–2019. doi: 10.1016/j.bbagen.2012.09.004. 

Wang Y, Pan H, Gouveia R, Zhao W, Shen Y, Kleene R, Costa J, Loers G, Schachner M (2012) Recombinant antibody fragments directed against different epitopes of the human neural cell adhesion molecule L1 act as inhibitors or activators of L1 function. PLOS One 7, e52404. doi: 10.1371/journal.pone.0052404.

Escrevente C, Grammel N, Kandzia S, Zeiser J, Tranfield EM, Conradt HS, Costa J (2013) Sialoglycoproteins and N-glycans from secreted exosomes of ovarian carcinoma cells. PLOS One 8(10): e78631. doi: 10.1371/journal.pone.0078631.

Carilho R, de Carvalho M, Swash M, Pinto S, Pinto A, Costa J (2014) Vascular endothelial growth factor and amyotrophic lateral sclerosis: The Interplay with exercise and non-invasive ventilation. Muscle Nerve 49:545-50. doi: 10.1002/mus.23955.

Lehnert S, Costa J, de Carvalho M, Kirby J, Kuzma-Kozakiewicz M, Morelli C, Robberecht W, Shaw P, Silani V, Steinacker P, Tumani H, van Damme P, Ludolph A, Otto M (2014) Multicentre quality control evaluation of different biomarker candidates for amyotrophic lateral sclerosis. Amyotrophic Lateral Sclerosis and Frontotemporal Degeneration 15:344-50. doi: 10.3109/21678421.2014.884592.