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Molecular Mechanisms of Pathogen Resistance

Research 

 

Selected Publications                     

Staphylococcus aureus       Fighting the innate immune system


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Carvalho SM, Freitas CS, Oliveira AS, and Saraiva LM (2021) Staphylococcus aureus flavohaemoglobin contributes to early stage biofilm development under nitrosative stress FEMS Microbiol. Lett., in press

Videira MAM, Lobo SAL, Sousa FL, Saraiva LM. (2020) FEBS J. Identification of the sirohaem biosynthesis pathway in Staphylococcus aureus. 287,1537-1553 

Videira MAM, Lobo SAL, Silva LSO, Palmer DJ, Warren MJ, Prieto M, Coutinho A, Sousa FL, Fernandes F, Saraiva LM. (2018) Staphylococcus aureus haem biosynthesis and acquisition pathways are linked through haem monooxygenase IsdG. Mol Microbiol. 109(3):385-400. 

Carvalho SM, de Jong A, Kloosterman TG, Kuipers OP, Saraiva LM. (2017) The Staphylococcus aureus α-Acetolactate Synthase ALS Confers Resistance to Nitrosative Stress. Front Microbiol. Jul 11;8:1273.

Lobo SAL, Scott A, Videira MAM, Winpenny D, Gardner M, Palmer MJ,  Schroeder S,  Lawrence A, Parkinson T, Warren MJ, and Saraiva LM (2015) Staphylococcus aureus haem biosynthesis: characterisation of the enzymes involved in final steps of the pathway. Mol. Microbiol., 97(3):472-87.

Nobre LS and Saraiva LM. (2013) Effect of combined oxidative and nitrosative stresses on Staphylococcus aureus transcriptome. Appl. Microbiol. Biotechnol. 97, 2563-73.

Nobre LS, Todorovic S, Tavares AFN, Oldfield P, Hildebrandt P, Teixeira M, and Saraiva, LM (2010) Binding of azole antibiotics to Staphylococcus aureus flavohemoglobin increases intracellular oxidative stress, J. Bacteriol. 192, 1527-33.

Tavares AFN, Nobre LS, Melo AMP, and Saraiva LM. (2009) A novel nitroreductase of Staphylococcus aureus with S-nitrosoglutathione reductase activity. J. Bacteriol. 191, 3403-3406.

   

       

Helicobacter species               Living in a nitrite rich environment

 

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Parente MR, Forte E, Falabella M, Boneca IG, Teixeira M, Giuffrè A, Saraiva LM. (2017) Nitrosative stress defences of the enterohepatic pathogenic bacterium Helicobacter pullorum. Sci Rep.  Aug 30;7(1):9909. 

Flint A, Stintzi A  Saraiva LM (2016) Oxidative and nitrosative stress defences of Helicobacter and Campylobacter species that counteract mammalian immunity. FEMS Microbiol Rev. Nov 1;40(6):938-960..

Parente MR, Monteiro JT, Martins GG, and Saraiva LM (2016) Helicobacter pullorum induces in murine macrophages nitric oxide release that promotes phagocytosis and killing, Microbiology. 162:503-12

Justino MC, Parente MR, Boneca IG, and Saraiva LM (2014) FrxA is a GSNO reductase enzyme that contributes to Helicobacter pylori pathogenicity. FEBS J. 281, 4495-4505.

Justino MC, Ecobichon C, Fernandes AF, Boneca IG, Saraiva LM. (2012) Helicobacter pylori has an unprecedented nitric oxide detoxifying system. Antioxid. Redox. Signal. 17, 1190-200.

 

Staphylococcus aureus 

Desulfovibrio spp.

Heme biosynthesis      

           

     

        

Zamarreno JB, Videira MAM , and Saraiva LM (2021) Regulation of bacterial haem biosynthesis. Coord. Chem. Reviews, in press.

Videira MAM, Lobo SAL, Sousa FL, Saraiva LM. (2020) FEBS J. Identification of the sirohaem biosynthesis pathway in Staphylococcus aureus. 287,1537-1553 

Videira MAM, Lobo SAL, Silva LSO, Palmer DJ, Warren MJ, Prieto M, Coutinho A, Sousa FL, Fernandes F, Saraiva LM. (2018) Staphylococcus aureus haem biosynthesis and acquisition pathways are linked through haem monooxygenase IsdG. Mol Microbiol. 109(3):385-400.

Lobo SA, Videira MA, Pacheco I, Wass MN, Warren MJ, Teixeira M, Matias PM, Romão CV, and Saraiva LM. 2017. Desulfovibrio vulgaris CbiKP cobaltochelatase: evolution of a haem binding protein orchestrated by the incorporation of two histidine residues. Environ Microbiol. 19, 106-118.

Lobo SAL, Scott A, Videira MAM, Winpenny D, Gardner M, Palmer MJ,  Schroeder S,  Lawrence A, Parkinson T, Warren MJ, and Saraiva LM (2015) Staphylococcus aureus haem biosynthesis: characterisation of the enzymes involved in final steps of the pathway. Mol. Microbiol., 97(3):472-87.

Lobo SAL, Lawrence AD, Romão CV, Warren MJ, Teixeira M, and Saraiva LM (2014) Characterization of Desulfovibrio vulgaris haem b synthase, a radical SAM family member. BBA Proteins and Proteomics, 1844, 1238-47.

Bali S, Lawrence AD, Lobo SA, Saraiva LM, Golding BT, Palmer DJ, Howard MJ, Ferguson SJ and Warren MJ. (2011) Molecular hijacking of siroheme for the synthesis of heme and d1 heme. Proc. Natl. Acad. Sci USA, 108, 18206-5.

Romão CV, Ladakis D, Lobo SA, Carrondo MA, Brindley AA, Deery E, Matias PM, Pickersgill MW, Saraiva LM and Warren MJ (2011) Evolution in a family of chelatases facilitated by the introduction of active site asymmetry and protein oligomerization. Proc. Natl. Acad. Sci. USA.108, 97-102.

 

        

Escherichia coli 

 

Nitrosative/oxidative stress defences against mammalian macrophages

 

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Silva LSO, Matias PM, Romão CV, Saraiva LM. (2021) Structural Basis of RICs Iron Donation for Iron-Sulfur Cluster Biogenesis. Front Microbiol. 12:670681.

Silva LSO, Baptista JM, Batley C, Andrews SC, Saraiva LM. ( 2018) The Di-iron RIC Protein (YtfE) of Escherichia coli Interacts with the DNA-Binding Protein from Starved Cells (Dps) To Diminish RIC Protein-Mediated Redox Stress. J Bacteriol. pii: e00527-18

Silva LO, Nobre LS, Mil-Homens D, Fialho A, Saraiva LM. (2018) Repair of Iron Centers RIC protein contributes to the virulence of Staphylococcus aureus. Virulence. 9:1, 312-317

Nobre LS,  Meloni D, Teixeira M, Viscogliosi E, and Saraiva LM (2016) Trichomonas vaginalis Repair of Iron Centres proteins: the different role of two paralogs. Protist. 167, 222–233. 

Nobre LS, Lousa D,  Pacheco I, Soares CM, Teixeira M and Saraiva LM (2015)  Insights into the structure of the diiron site of RIC from Escherichia coli. FEBS Lett. 589 (4):426-431.

Nobre LS, Garcia-Serres R, Todorovic S, Hildebrandt P, Teixeira M,  Latour JM,  and Saraiva LM. (2014) Escherichia coli RIC is able to donate iron to iron-sulfur clusters. PLOS One , 16, 9(4), e95222.

Justino MC, Almeida CC, Teixeira M, and Saraiva LM. (2007) Escherichia coli di-iron YtfE protein is necessary for the repair of stress damaged iron-sulfur clusters. J. Biol. Chem. 282, 10352-10359.

 

CORMs 

 

 

New antibiotics

 

Mendes SS, Miranda V, Saraiva LM. (2021) Antioxidants (Basel). Hydrogen Sulfide and Carbon Monoxide Tolerance in Bacteria. 10, 729.

Carvalho SM, Marques J, Romão CC, Saraiva LM (2019) Metabolomics of Escherichia coli Treated with the Antimicrobial Carbon Monoxide-Releasing Molecule CORM-3 Reveals Tricarboxylic Acid Cycle as Major Target. Antimicrob Agents Chemother. pii: e00643-19

Nobre LS, Jeremias H, Romão CC and Saraiva LM (2016) Examining the antimicrobial activity and toxicity to animal cells of different types of CO-releasing molecules,  Dalton Trans. 45,1455-66.

Tavares AF, Parente MR, Justino MC, Oleastro M, Nobre LS, and Saraiva LM. (2013) The bactericidal activity of carbon monoxide-releasing molecules against Helicobacter pylori. PLoS One. 8(12):e83157.

Tavares AF, Teixeira M, Romao CC, Seixas JD, Nobre LS, and Saraiva LM. (2011) Reactive oxygen species mediate bactericidal killing elicited by carbon monoxide-releasing molecules. J. Biol Chem. 286, 26708-17. 

Nobre LS, Seixas JD, Romão CC, and Saraiva LM. (2007) The antimicrobial action of carbon monoxide releasing compounds. Antimicrob. Agents Chemother. 51, 4303-07.

     

    

 


Keywords

 

           

Staphylococcus aureus, Helicobacter pylori, Desulfovibrio, sulphate-reducing bacteria, Escherichia coli;

Bacteria, anaerobic microorganisms, pathogen, antibiotic, antibiotic resistance, immune system, carbon monoxide, iron metabolism, heme;

Cell culture, molecular genetics, genomics, transcriptomics, microbiology, biochemistry.

 

 

See all publications

Pubmed Link

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Group Members

 

      

Lígia M. Saraiva

Head of Laboratory

Habilitacion (Agregação), ITQB-Universidade Nova de Lisboa 

Ph.D in Biochemistry - Biochemical Physics, Universidade Nova de Lisboa
Degree in Chemical Engineering, Instituto Superior Técnico

Phone: 214469328.
E-mail: lst@itqb.unl.pt

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Sandra Carvalho

Post-Doctoral Researche

Ph.D in Biochemistry, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa

Degree in Biochemistry, Faculdade de Ciências e Tecnologia da Universidade de Coimbra

Phone: 214469329. E-mail: smcc@itqb.unl.pt

Discover novel mechanisms of resistance to nitrosative stress

   

Marco Videira

Post-Doctoral Researcher

Ph.D in Biochemistry, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa

Master in Biomedical Sciences, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa

Degree in Biochemistry, Faculdade de Ciências e Tecnologia da Universidade Nova de Lisboa

Phone: 214469329. E-mail: m.videira@itqb.unl.pt

Tetrapyrrole biosynthesis in Staphylococcus aureus

 

 Zamarreno Beas

Post-Doctoral Researcher

Ph.D in Microbiology, Centre Nationale de Recherche Scientific, Université Aix-Marseille

Degree In Microbiology Biotechnology and Vegetal Biology, Université Aix-Marseille

Phone: 214469329. E-mail: jzamarreno@itqb.unl.pt

Tetrapyrrole biosynthesis in Campylobacter jejuni

 

Liliana Silva

Ph.D student

Master in Clinical Analysis, Faculdade de Farmácia da Universidade de Coimbra

Degree in Bioanalytical Sciences, Faculdade de Farmácia da Universidade de Coimbra

Phone: 214469329. E-mail: lsilva@itqb.unl.pt

Study of RIC proteins of Escherichia coli and Staphylococcus aureus 

   

Ana Sofia Mendes

Ph.D student 

Master in Biochemistry for Health, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa

Degree in Cellular and Molecular Biology, Faculdade de Ciências e Tecnologia da Universidade Nova de Lisboa

Phone: 214469329. E-mail: ass.mendes@itqb.unl.pt

Study of the antimicrobial properties of carbon monoxide-releasing molecules (CORMs)

   

Joana Sofia Monteiro Batista

Ph.D student

Master in Translational Cardiovascular Medicine, University of Bristol, United Kingdom

Degree in Clinical Analysis and Public Health, Escola Superior de Tecnologias de Lisboa (ESTEsL), Portugal

Phone: 214469329. E-mail: joana.batista@itqb.unl.pt

Study of tetrapyrrole homeostasis in gut pathogens

 

 

Ana Rita Oliveira

Ph.D student

Master in Biotechnology, Faculdade de Ciências e Tecnologia da Universidade Nova de Lisboa

Degree in Biomedical Sciences, Universidade de Aveiro

Phone: 214469329. E-mail: ars.oliveira@itqb.unl.pt

Study of the resistance of S. epidermidis to nitrosative stress

   

 

     

 

Former members

Jacqueline Fernandes, Cristiano Conte, Alexandra Lourenço, Paula Lourenço, Helena Duarte, Rute Valente, Susana Pascoal, Patricia Noronha da Costa (PhD), Claudia Almeida, Vera Gonçalves (PhD), Joana Baptista (PhD), Ana Filipa Tavares (PhD), Marta Campos Justino (PhD), Mafalda Figueiredo (PhD), Sara Sousa, Sofia Costa, Vera Mónica Gonçalves, Nuno Carvalho, Mafalda Barros, Catarina Godinho, Luis Sobral, Fábio Pereira, Cláudia Ribeiro, Ana Antão, Inês Ladeira, Inês Santos, Roberta Benedetto, Lígia Nobre, Margarida Parente, Susana Lobo, Patrícia Ferreira, Cátia Família, Joana Marques, Cláudia Freitas.

 

Group and Lab Photos

  

 

             

                            December 2020

 

 

May 2018

   January 2020

 

June 2016

Christmas 2017

                                               

                      Vimeiro, Portugal 15-18th March 2015 

Bacterial Electron Transfer Processes and their Regulation

                            June 2016                                                                            

 

 Christmas 2014  

 

Christmas 2014

June 2014

March 2013

June 2012

February 2011

September 2010

September 2006

September 2013

April 2013

July 2012

June 2011

November 2010

July 2009

 

July 2008

12-22nd March 2006

Meeting on Microbial Respiratory Chains

 

 

July 2008

 

12-22nd March 2006

Meeting on Microbial Respiratory Chains

 

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