Physicochemical characterization of small drug molecules by capillary electrophoresis
The physicochemical properties of pharmaceuticals such as acid dissociation constant (pKa), octanol-water partition coefficiente (log Pow), solubility or protein binding constant are very important in drug design, candidate selection, drug delivery and pharmacokinetic studies. Capillary electrophoresis (CE) is a simple, versatile, automated, and powerful separation technique and widely applied in physicochemical profiling for pharmaceuticals. It has advantages over other methodologies, such as high separation efficiency, short analysis time and low sample and electrolyte (buffer) consumption.
In our laboratory capillary electrophoresis is routinely used for the establishment of physicochemical properties of pharmaceuticals and have been applied on the determination of the ionization profiles of flavonoids, amines prodrugs and others small drug molecules.
