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Publications

 

Ferreira AC, Rebelo BA, Abranches R. (2023). A simplified protocol for Agrobacterium-mediated transformation of cell suspension cultures of the model species Medicago truncatula A17. Plant Cell, Tissue and Organ Culture (PCTOC). DOI: 10.1007/s11240-023-02495-6

 

Rebelo BA, Folgado A, Ferreira AC, Abranches R. (2022). Production of the SARS-CoV-2 Spike protein and its Receptor Binding Domain in plant cell suspension cultures. Front. Plant Sci. 13:995429. doi: 10.3389/fpls.2022.995429

 

Afonso C, Bragança AR, Rebelo BA, Serra TS, Abranches R. (2022). Optimal Nitrate Supplementation in Phaeodactylum tricornutum Culture Medium Increases Biomass and Fucoxanthin Production. Foods 2022, 11(4) 568; https://doi.org/10.3390/foods11040568

 

Folgado A, Abranches R. (2021). Tobacco BY2 cells expressing recombinant cardosin B as an alternative for production of active milk clotting enzymes. Scientific Reports  11, 14501. https://doi.org/10.1038/s41598-021-93882-6

 
 
Lobato Gomez et al. (2021). Contributions of the international plant science community to the fight against human infectious diseases – part 1: epidemic and pandemic diseases. Plant Biotechnology Journal. Published June 28. https://doi.org/10.1111/pbi.13657
 
 
He et al. (2021). Contributions of the international plant science community to the fight against infectious diseases in humans – part 2: affordable drugs in edible plants for endemic and re‐emerging diseases. Plant Biotechnology Journal. Published June 28. https://doi.org/10.1111/pbi.13658
 
 
Folgado A, Serra, AT, Prazeres I, Bento-Silva A, Bronze MR, Abranches R (2021). Hairy roots cultures of Cynara cardunculus L. as a valuable source of hydroxycinnamic acid compounds. Plant Cell, Tissue and Organ Culture (PCTOC) 147, 37–47. DOI: 10.1007/s11240-021-02102-6
 
 
Rebelo BA, Farrona S, Ventura MR, Abranches R (2020). Canthaxanthin, a Red-Hot Carotenoid: Applications, Synthesis, and Biosynthetic Evolution. Plants 9, 1039. https://doi.org/10.3390/plants9081039
 
 
Folgado A, Abranches R (2020). Plant Aspartic Proteases for Industrial Applications: Thistle Get Better. Plants 9, 147. DOI:10.3390/plants9020147 
 
 
Rebelo B, Santos RB, Ascenso O, Nogueira AC, Lousa D, Abranches R#, Ventura MR#. (2020). Synthesis and biological effects of small molecule enhancers for improved recombinant protein production in plant cell cultures. Bioorganic Chemistry 94, 103452. DOI: 10.1016/j.bioorg.2019.103452
 
 
Folgado A, Pires AS, Figueiredo AC, Pimentel C, Abranches R. (2020). Toward alternative sources of milk coagulants for cheese manufacturing: establishment of hairy roots culture and protease characterization from Cynara cardunculus L. Plant Cell Reports 39, 89–100. DOI: 10.1007/s00299-019-02475-1.
 
 
Folgado A & Abranches R. (2019). O cardo e a indústria de queijo: ferramentas biotecnológicas para a produção de enzimas utilizadas na coagulação do leite. Revista de Ciências Agrárias 42, 817-828. DOI: 10.19084/rca.17565.
 
 
Santos RB, Pires AS, van der Hoorn RAL, Schiermeyer A, Abranches R. (2019). Generation of transgenic cell suspension cultures of the model legume Medicago truncatula: A rapid method for Agrobacterium mediated gene transfer. Plant Cell, Tissue and Organ Culture (PCTOC) 136, 445-450. DOI:10.1007/s11240-018-1525-3. 
 
 
Santos RB, Chandra B, Mandal MK, Kaschani F, Kaiser M, Both L, van der Hoorn RAL, Schiermeyer A, Abranches R. (2018). Low protease content in Medicago truncatula cell cultures facilitates recombinant protein production. Biotechnology Journal 13(7):e1800050. doi: 10.1002/biot.201800050. 
 
 
Perfeito C, Ambrosio M, Santos RB, Afonso CN, Abranches R. (2018). Increasing fucoxanthin production in Phaeodactylum tricornutum using genetic engineering and optimization of culture conditions. Frontiers in Marine Science IMMR2018. DOI: 10.3389/conf.FMARS.2018.06.00082
 
 
Santos RB, Pires AS and Abranches R. (2017). Addition of a histone deacetylase inhibitor increases recombinant protein expression in Medicago truncatula cell cultures. Scientific Reports 7: 16756. DOI:10.1038/s41598-017-17006-9 
 
 

Santos RB, Abranches R, Fischer R, Sack M and Holland T. (2016). Putting the spotlight back on plant suspension cultures. Frontiers in Plant Science 7:297. doi: 10.3389/fpls.2016.00297

 

Pires AS, Azinheira HG, Cabral A, Tavares S, Tavares D, Castro M, Varzea V, Silva MC, Abranches R, Loureiro J and Talhinhas P. (2016). Cytogenomic characterisation of Colletotrichum kahawae, the causal agent of Coffee Berry Disease, reveals diversity in minichromosome profiles and genome size expansion. Plant Pathology 65, 968–977 DOI: 10.1111/ppa.12479

 

Santos AP, Abranches R, Oliveira MM and Shaw P. (2015) Plasticity of Chromatin Organization in the Plant Interphase Nucleus. In "Nuclear Functions in Plant Transcription, Signaling and Development”, Springer New York, pp. 57-79.

 

Rosa S, Ntoukakis V, Ohmido N, Pendle A, Abranches R and Shaw P. (2014). Cell differentiation and development in Arabidopsis are associated with changes in histone dynamics at the single cell level. The Plant Cell 26: 4821–4833. doi: http://dx.doi.org/10.1105/tpc.114.133793

 

Tavares S, Ramos AP, Pires AS, Azinheira HG, Caldeirinha P, Link T, Abranches R, Silva MC, Voegele RT, Loureiro J and Talhinhas P. (2014). Genome size analyses of Pucciniales reveal the largest fungal genomes. Frontiers in Plant Science 5:422. doi: 10.3389/fpls.2014.00422

 

Pires AS, Santos RB, Nogueira AC and Abranches R. (2014). Production of human Lipocalin-type Prostaglandin D synthase in the model plant Medicago truncatula. In Vitro Cellular and Developmental Biology 50, 276-281.DOI276-281. DOI: 1007/s11627-013-9584-y

 

Pires AS, Rosa S, Castanheira S, Fevereiro P and Abranches R. (2012). Expression of a recombinant human Erythropoietin in suspension cell cultures of Arabidopsis, Tobacco and Medicago. Plant Cell, Tissue and Organ Culture 110, 171-181. DOI:10.1007/s11240-012-0141-x

 

Pires AS, Geraldo N, Cerqueira T and Abranches R. (2012). Integrated approaches to studying Medicago truncatula genome structure and function and their applications in biotechnology. Molecular Breeding 30, 1431-1442. DOI:10.1007/s11032-012-9729-4

 

Santos RB, Pires AS, Silva HS and Abranches R. (2012). Assessment of Medicago based systems for the production of human proteins: Microscopy analysis of the subcellular deposition patterns of the recombinant product. Microscopy and Microanalysis 18S5, 11-12. doi:10.1017/S1431927612012718

 

Gonzalez-Melendi P, Pires AS and Abranches R. (2009). Cell line-dependent sorting of recombinant phytase in cell cultures of Medicago truncatula. Functional Plant Biology 36, 431-441. DOI: 10.1071/FP08260

 

Geraldo N and Abranches R. (2008). Immunolocalization of histone modifications as a tool to visualize chromatin dynamics in plants. Microscopy and Microanalysis 14S3, 130-133. DOI 10.1017/S1431927608089642

 

Pires AS, Cabral MG, Fevereiro P, Stoger E and Abranches R. (2008). High levels of stable phytase accumulate in the culture medium of transgenic Medicago truncatula cell suspension cultures. Biotechnology Journal 3, 916-923. DOI 10.1002/biot.200800044

 

Abranches R, Arcalis E, Marcel S, Altmann F, Ribeiro-Pedro M, Rodriguez J and Stoger E. (2008). Functional specialization of Medicago truncatula leaves and seeds does not affect the subcellular localization of a recombinant protein. Planta 227, 649-58. DOI 10.1007/s00425-007-0647-3.

 

Santos AP, Wegel E, Allen GC, Thompson WF, Stoger E, Shaw P and Abranches R. (2006). In situ methods to localize transgenes and transcripts in interphase nuclei: a tool for transgenic plant research. Plant Methods 2006, 2:18 DOI 10.1186/1746-4811-2-18

 

Kohli A, Gonzalez-Melendi P, Abranches R, Capell T, Stoger E and Christou P. (2006). The quest to understand the basis and mechanisms that control expression of introduced transgenes in crop plants. Plant Signaling & Behavior 1, 185-195.

 

Cabral G, Pires AS, González-Melendi P and Abranches R.(2006). Characterization of Medicago truncatula cell suspension cultures producing valuable recombinant proteins. Microbial Cell Factories 5 (Suppl 1):P92. doi:10.1186/1475-2859-5-S1-P92

 

Abranches R, Marcel S, Arcalis E, Altmann F, Fevereiro P and Stoger E. (2005). Plants as bioreactors: a comparative study suggests that Medicago truncatula is a promising production system. Journal of Biotechnology 120, 121-134.

 

Abranches R, Shultz RW, Thompson WF and Allen GC. (2005). Matrix Attachment Regions and regulated transcription increase and stabilize transgene expression. Plant Biotechnology Journal 3, 535-543.

 

Kohli A, Twyman RM, Abranches R, Wegel E, Stoger E and Christou P. (2003) Transgene integration, organization and interaction in plants. Plant Molecular Biology 52, 247-258.

 

Shaw PJ, Abranches R, Santos AP, Beven AF, Stoger E, Wegel E and Gonzalez-Melendi P. (2002). The architecture of interphase chromosomes and nucleolar transcription sites in plants. Journal of Structural Biology 140, 31-38.

 

Callaway AS, Abranches R, Scroggs J, Allen GC and Thompson WF. (2002). High-throughput transgene copy number estimation by competitive PCR. Plant Molecular Biology Reporter 20, 265-277.

 

Santos AP, Abranches R, Stoger E, Beven A, Viegas W and Shaw PJ. (2002). The architecture of interphase chromosomes and gene positioning are altered by changes in DNA methylation and histone acetylation. Journal of Cell Science 115, 4597-4605.

 

Abranches R, Santos AP, Wegel E, Williams S, Castilho A, Christou P, Shaw P and Stoger E. (2000). Widely separated multiple transgene integration sites in wheat chromosomes are brought together at interphase. The Plant Journal 24, 713-723.

 

Gonzalez-Melendi P, Beven A, Boudonck K, Abranches R, Wells B, Dolan L and Shaw P. (2000). The nucleus: a highly organized but dynamic structure. Journal of Microscopy 198, 199-207.

 

Abranches R, Beven AF, Aragon-Alcaide L and Shaw PJ. (1998). Transcription sites are not correlated with chromosome territories in wheat nuclei. Journal of Cell Biology 143, 5-12.

 

Abranches R and  Shaw P. (1998). Transcription sites and chromosome domains. Molecular Biology of the Cell 9, 2613.

 

Amado L, Abranches R, Neves N and Viegas W. (1997). Development-dependent inheritance of 5-azacytidine induced epimutations in triticale: analysis of rDNA expression patterns. Chromosome Research 5: 445-450.

 

 

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