Publications
Papers in international scientific periodicals with referees
2013
50 – A. Barbas, A. O. Popescu, C. Frazão, C. M. Arraiano, A. M. Fialho, “Rossmann-fold motifs can confer multiple functions to metabolic enzymes: RNA binding and ribonuclease activity of a UDP-glucose dehydrogenase” BBRC (2013) 430:218-224.
49 - T.M. Bandeiras, M.C. Freitas, D. Petrasch, A. Kletzin, C. Frazao, "SAD phasing towards structure determination of a thermostable Rieske ferredoxin with a novel stabilizing disulfide bridge" Acta Crystallog (2010) F66: 269-271
2012
48 - L. Pereira, I. H. Saraiva, R. Coelho, D. K. Newman, R. O. Louro and C. Frazão, “Crystallization and preliminary crystallographic studies of FoxE from Rhodobacter ferrooxidans SW2, an FeII oxidoreductase involved in photoferrotrophy” Acta Crystallog (2012) F69: 555-558.
2011
47 - J. Rocha, A. O. Popescu, P. Borges, D. Mil-Homens, L. M. Moreira, I. Sá-Correia, A. M. Fialho, C. Frazão, "Structure of Burkholderia cepacia UDP-glucose dehydrogenase (UGD) BceC and role of Tyr10 in final hydrolysis of UGD thioester intermediate" J. Bacteriology (2011) 193(15): 3978-3987.
46 - A. Veith, T. Urich, K. Seyfarth, J. Protze, C. Frazao, A. Kletzin, "Substrate pathways and mechanisms of inhibition in the sulfur oxygenase reductase of Acidianus ambivalens" Frontiers in Microbiology (2011) 2(37):1-12 http://dx.doi.org/10.3389/fmicb.2011.00037
2010
45 - J. Rocha, A. O. Popescu, I. Sá-Correia, A. M. Fialho, C. Frazao, "Cloning, Expression, Purification, Crystallization and Preliminary Crystallographic Studies of BceC, a UDP-glucose Dehydrogenase from Burkholderia cepacia IST 408" Acta Crystallog (2010) F66: 269-271.
44- J. Rocha, A. T. Granja, I. Sá-Correia, A. M. Fialho, C. Frazao, "Cloning, expression, purification, crystallization and preliminary crystallographic studies of UgdG, an UDP-glucose dehydrogenase from Sphingomonas elodea ATCC 31461" Acta Crystallog (2010) F66: 69-72.
2008
43 – M. R. Freixo, A. Karmali, C. Frazão, J. M. Arteiro, “Production of laccase and xylanase from Coriolus versicolor grown on tomato pomace and their chromatographic behaviour on immobilized metal chelates” Proc Biochemistry (2008) 43, 1265-1274.
42 – D. Aragão, E. P. Mitchell, C. Frazão, M. A. Carrondo, P. F. Lindley, “Structural and functional relationships in the hybrid cluster protein family: structure of the anaerobically purified hybrid cluster protein from Desulfovibrio vulgaris at 1.35 Å resolution” Acta Crystallog (2008) D64, 665-674.
41 - C. Frazão, D. Aragão, R. Coelho, S. S. Leal, C. M. Gomes, M. Teixeira, M. A. Carrondo, “Crystallographic analysis of the intact metal centres [3Fe4S]1+/0 and [4Fe4S]2+/1+ in a Zn2+-containing ferredoxin” FEBS Letters (2008) 582, 763-767.
2007
40 - J. Andrade, A. Karmali, M. A. Carrondo, C. Frazão, "Structure of Amidase from Pseudomonas aeruginosa Showing a Trapped Acyl Transfer Reaction Intermediate State" JBC (2007) 282, 18598-19605.
39 - J. Andrade, A. Karmali, M. A. Carrondo, C. Frazão, "Crystallization, diffraction data collection and preliminary crystallographic analysis of hexagonal crystals from Pseudomonas aeruginosa amidase", Acta Crystallog (2007) F63, 214-216.
38 - D. Aragão, A. F. Fialho, A. R. Marques, E. P. Mitchell, I. Sá-Correia, C. Frazão, "The Complex of Sphingomonas eloedea ATCC 31461 Glucose-1-phosphate Uridylyltransferase with Glucose-1-Phosphate reveals a Novel Quaternary Structure, Unique among Nucleoside Diphosphate-Sugar Pyrophosphorylase Members" J. Bacteriology (2007) 189, 4520-4528.
2006
37 - D. Aragão, A. R. Marques, C. Frazão, F. J. Enguita, M. A. Carrondo, A. M. Fialho, I. Sá-Correia, E. P. Mitchell, " Cloning, expression, purification, crystallization and preliminary structure determination of glucose-1-phosphate uridylyltransferase (UgpG) from Sphingomonas elodea ATCC 31461 bound to glucose-1-phosphate", Acta Crystallog (2006) F62, 930-934.
36 - C. Frazão, C. E. McVey, M. Amblar, A. Barbas, C. Vonrhein, C. M. Arraiano, M. A. Carrondo, "Unravelling the dynamics of RNA degradation by RNase II and its RNA-bound complex", Nature (2006) 443, 110-114.
35 - C. E. McVey, M. Amblar, A. Barbas, F. Cairrao, R. Coelho, C. Romao, C.M. Arraiano, M. A. Carrondo, C. Frazao, "Expression, purification, crystallization and preliminary diffraction data characterization of Escherichia coli ribonuclease II (RNase II)", Acta Crystallog (2006) F62, 684-687.
34 - T. Urich, C.M. Gomes, A. Kletzin, C. Frazao, "X-ray Structure of a self-compartmentalizing sulfur cycle metalloenzyme", Science (2006) 311, 996-1000.
2005
33 - T. Urich, R. Coelho, A. Kletzin, C. Frazao, "The Sulfur Oxygenase Reductase from Acidianus ambivalens is an icosatetramer as shown by Crystallization and Patterson analysis", Biochimica et Biophysica Acta (2005) 1747, 267-270.
32 - C. Frazao, Dina C. Simes, R. Coelho, D. Alves, M. K. Williamson, P. A. Price, M. L. Cancela, M. A. Carrondo, "Structural Evidence of a Fourth Gla Residue in Fish Osteocalcin: Biological Implications", Biochemistry (2005) 44, 1234-1242.
2004
31 - I. J. Correia, C. M. Paquete, A. Coelho, C. C. Almeida, T. Catarino, R. O. Louro, C. Frazão, L. M. Saraiva, M. A. Carrondo, D. L. Turner, A. V. Xavier "Proton-assisted two-electron transfer in natural variants of tetraheme cytochromes from Desulfomicrobium Sp.", JBC (2004) 279, 52227-52237.
2003
30 - D. Aragão, S. Macedo, E. P. Mitchell, C. V. Romão, M. Y. Liu, C. Frazão, L. M. Saraiva, A. V. Xavier, J. LeGall, W. M. van Dongen, W. R. Hagen, M. Teixeira, M. A. Carrondo, P. Lindley, "Reduced hybrid cluster proteins (HCP) from Desulfovibrio desulfuricans ATCC 27774 and Desulfovibrio vulgaris (Hildenborough): X-ray structures at high resolution using synchrotron radiation", JBIC (2003) 8, 540-548.
29 - B. L. Victor, J. B. Vicente, R. Rodrigues, S. Oliveira, C. Rodrigues-Pousada, C. Frazão, C. M. Gomes, M. Teixeira, C. M. Soares, "Docking and electron transfer studies between rubredoxin and rubredoxin:oxygen oxidoreductase", JBIC (2003) 8, 475-488.
28 - D. Aragão, C. Frazão, L. Sieker, G. M. Sheldrick, J. LeGall, M. A. Carrondo, "Crystal structure of the di-tetraheme cytochrome c3 from Desulfovibrio gigas at 1.2 Å resolution" Acta Crystallog (2003) D59, 644-653.
2002
27 - C. M. Gomes, C. Frazão, A. V. Xavier, J. Legall, M. Teixeira, "Functional control of the binuclear metal site in the metallo-beta-lactamase-like fold by subtle amino acid replacements", Protein Science (2002) 11, 707-712.
2001
26 - C. Frazão, F. J. Enguita, R. Coelho, G. M. Sheldrick, J. A. Navarro, M.Hervás, M. A. De la Rosa, M. A. Carrondo, "Crystal structure of low-potential cytochrome c549 from Synechocystis sp. PCC 6803, at 1.21 Å resolution" JBIC (2001) 6, 324-332.
2000
25 - C. Frazão, G. Silva, C. M. Gomes, P. Matias, R. Coelho, L. Sieker, S. Macedo, M. Y. Liu, S. Oliveira, M. Teixeira, A. V. Xavier, C. Rodrigues-Pousada, M. A. Carrondo, J. Le Gall, "Dioxygen reduction in strict anaerobes, structure of Desulfovibrio gigas rubredoxin:oxygen oxidoreductase", Nat Struct Biol (2000) 7(11), 1041-1045.
1999
24 - C. Frazão, L. Sieker, R. Coelho, J. Morais, I. Pacheco, L. Chen, J. LeGall, Z. Dauter, K. Wilson, M. A. Carrondo, "Crystallization and preliminary diffraction data analysis of both single and pseudo-merohedrally twinned crystals of rubredoxin oxygen oxidoreductase from Desulfovibrio gigas". Acta Crystallog (1999) D55, 1465-1467.
23 - C. Frazão, L. Sieker, G. Sheldrick, V. Lamzin, J. LeGall, M. A. Carrondo, "Ab initio structure solution of a dimeric cytochrome c3 from Desulfovibrio gigas containing disulphide bridges". JBIC (1999) 4, 162-165.
22 - C. Frazão, I. Bento, J. Costa, C. M. Soares, P. Veríssimo, C. Faro, E. Pires, J. Cooper, M. A. Carrondo. "Crystal structure of cardosin A, a glycosylated and Arg-Gly-Asp-containing aspartic proteinase from the flowers of Cynara cardunculus L.", JBC (1999) 274(39), 27694-27701.
1998
21 - I. Bento, C. Frazão, R. Coelho, K. Wilson, Z. Dauter, M. A. Carrondo, "Crystallization and preliminary X-ray crystallographic studies of the plant aspartic proteinase cardosin A". Acta Crystallog (1998) D54, 991-993.
1997
20 - J. Costa, D. A. Ashford, M. Nimtz, I. Bento, C. Frazão, C. L. Esteves, C. J. Faro, J. Kervinen, E. Pires, P. Veríssimo, A. Wlodawer, M. A. Carrondo, "The glycosylation of the aspartic proteinases from barley (Hordeum vulgare L.) and cardoon (Cynara cardunculus L.)", Eur J Biochem (1997) 243, 695-700.
1995 - 1984
19 - Frazão, C. M. Soares, M. A. Carrondo, E. Pohl, Z. Dauter, K. S. Wilson, M. Hervás, J. A. Navarro, M. De la Rosa, G. M. Sheldrick, “Ab initio determination of the crystal structure of cytochrome c6. Comparison with plastocyanin”, Structure (1995) 3, 1159-1169.
18 - Morais, P. N. Palma, C. Frazão, J. Caldeira, J. LeGall, I. Moura, J. G. Moura, M. A. Carrondo, “Structure of the tetraheme cytochrome from Desulfovibrio desulfuricans ATCC 27774: X-ray diffraction and electron paramagnetic resonance studies”, Biochemistry (1995) 34, 12830-12841.
17 - C. Frazão, J. M. Dias, P. M. Matias, M. J. Romão, M. A. Carrondo, M. Hervás, J. A. Navarro, M. De la Rosa, G. M. Sheldrick, "Cytochrome c6 from the green alga Monoraphidium braunii. Crystallisation and preliminary diffraction studies", Acta Crystallog (1995) D51, 232-234.
16 - C. Frazão, J. Morais, P. M. Matias, M. A. Carrondo, "Crystallization and Preliminary X-Ray Diffraction Analysis of Tetra-heme Cytochrome c3 from Sulfate and Nitrate Reducing Desulfovibrio desulfuricans ATCC 27774", Acta Crystallog (1994), D50, 233-236.
15 - C. G. Dealwis, C. Frazão, M. Badasso, J. B. Cooper, I. J. Tickle, H. Driessen, T. L. Blundell, K. Murakami, H. Miyazaki, J. Sueiras-Diaz, D. M. Jones, M. Szelke, "X-ray analysis at 2.0 Å resolution of mouse submaxillary renin complexed with a decapepetide inhibitor CH-66, based on the 4-16 fragment of rat angiotensinogen", J Mol Biol (1994) 236, 342-360.
14 - C. Frazão, C. Topham, V. Dhanaraj, T. L. Blundell, "Comparative modelling of human renin: a retrospective evaluation of the model with respect to the X-ray crystal structure", Protein structure-function relationship, (Z. H. Zaidi, A. Abbasi, D. L. Smith, editors) , TWEL Publishers, 1993, 1-13, and Pure Appl Chem (1994) 66, 43-50.
13 - P. M. Matias, C. Frazão, J. Morais, M. Coll, M. A. Carrondo, "Structure Analysis of Cytochrome c3 from Desulfovibrio vulgaris Hildenborough at 1.9Å Resolution", J Mol Biol, (1993) 234, 680-699.
12 - J. Cooper, W. Quail, C. Frazão, S. I. Foundling, T. L. Blundell, C. Humblet, E. A. Lunney, W. T. Lowther, B. M. Dunn, "X-ray crystallographic analysis of inhibition of endothiapepsin by cyclohexyl renin inhibitors", Biochemistry (1992) 31, 8142-8150.
11 - V. Dhanaraj, C. Dealwis, C. Frazão, M. Badasso, I. J. Tickle, J. B. Cooper, M. Newman, C. Aguilar, S. P. Wood, T. L. Blundell, P. M. Hobart, K. F. Geoghegan, M. J. Ammirati, D. E. Danley, B. A. O´Connor, D. J. Hoover, "Molecular Recognition and Drug Design: The Structural Basis of Specificity of Human and Mouse Renins Defined by X-Ray Analysis of Peptide Inhibitor Complexes" in ´Molecular Recognition: Chemical and Biochemical Problems II´, edited by S. M. Roberts, Special Publication Nº.111, The Royal Society of Chemistry, Cambridge 1992.
10 - V. Dhanaraj, C. G. Dealwis, C. Frazão, M. Badasso, B. L. Sibanda, I. J. Tickle, J. B. Cooper, H. P. C. Driessen, M. Newman, C. Aguilar, S. P. Wood, T. L. Blundell, P. M. Hobart, K. F. Geoghegan, M. J. Ammirati, D. E. Danley, B. A. O´Connor, D. J. Hoover, "X-ray analysis of peptide-inhibitor complexes define the structural basis of specificity for human and mouse renins", Nature (1992) 357, 466-472.
9 - M. Badasso, C. Frazão, B. L. Sibanda, V. Dhanaraj, C. Dealwis, J. B. Cooper, S. P. Wood, T. L. Blundell, K. Murakami, H. Miyazaki, P. M. Hobart, K. F. Geoghegan, M. J. Ammirati, A. L. Lanzetti, D. E. Danley, B. A. O´Connor, D. J. Hoover, J. Sueiras-Diaz, D. M. Jones, M. Szelke, "Crystallization and Preliminary X-ray Analysis of Complexes of Peptide Inhibitors with Human Recombinant and Mouse Submandibular Renins", J Molecular Biology (1992) 223, 447-453.
8 - C. M. Topham, E. Salih, C. Frazão, D. Kowlessur, J. P. Overington, M. Thomas, S. M. Brocklehurst, M. Patel, E. W. Thomas, K. Brocklehurst, "Structure-function relationships in the cysteine proteinases actinidin, papain and papaya proteinase omega. Three-dimensional structure of papaya proteinase omega deducedby knowledge-based modelling and active-centre reactivity probe kinetics and kinetics of catalysis", Biochem J (1991) 280 (Pt. 1), 79-92.
7 - M. Newman, M. Safro, C. Frazão, G. Khan, A. Zdanov, I. Tickle, T. L. Blundell, N. Andreeva, "X-Ray Analysis of Aspartic Proteases IV. Structure and refinement at 2.2 Å of Bovine Chymosin", J Molecular Biology (1991) 221,1295-1309.
6 - P. Strop, J. Sedlacek, J. Stys, Z. Kaderabkova, I. Blaha, L. Pavlickova, J. Pohl, M. Fabry, V. Kostka, M. Newman, C. Frazão, A. Shearer, I. J. Tickle, T. L. Blundell, "Engineering Enzyme Sub-site Specificity: Preparation, Kinetic, Characterisation and X-ray Analysis at 2.0 Å of Val111Phe Site-mutated Calf Chymosin", Biochemistry (1990) 29, 9863-71.
5 - D. G. Anderson, D. W. H. Rankin, H. E. Robertson, C. M. Frazão, H. Schmidbaur, "The Synthesis and Molecular Structure of Tetra(isopropyl)silane", Chem Ber (1989) 122, 2213-2218.
4 - H. Schmidbaur, C. M. Frazão, G. Reber, G. Müller, "Contributions to the synthesis and structural chemistry of some alkenyl- and allenylphosphines", Chem Ber (1989) 122 (2), 259.
3 - M. J. Calhorda, M. A. A. Carrondo, A. R. Dias, C. F. Frazão, M. B. Hursthouse, J. A. M. Simões, C. Teixeira, "Energetics of Metal-Sulfur Bonds in the Complexes M(eta5-C5H5)2(SC2H5)2 (M = Ti, W) and W(eta5-C5H5)2(SC6H5)2. Molecular Structure of Ti(eta5-C5H5)2(SC2H5)2", Inorg Chem J (1988) 27, 2513-2518.2
2 - H. Schmidbaur, A. Schier, C. M. F. Frazão, G. Müller, "Structural Correlations between the Tetraisopropylphosphonium Cation and the Triisopropylphosphonium Isopropylide. An X-Ray Study", JACS (1986) 108, 976-982.
1 - M. J. Calhorda, C. F. Frazão, J. A. M. Simões, "Metal-Carbon ´Bond-Strengths´ in Cr(CO)6, Cr(eta-C6H6)2, and Cr(CO)3(eta-C6H6)", J Organomet Chem (1984) 262, 305-314.
