Research Facilities
ITQB NOVA Technicians
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Catarina Correia |
Conceição Almeida |
Cristina Leitão |
Cristina Timóteo |
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Helena Matias |
João Carita |
Pedro Lamosa |
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Teresa B. da Silva |
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Acknowledgment Research Services
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NMR Facility CERMAX - facility video ITQB NOVA hosts the largest Portuguese NMR facility - Centro de Ressonância Magnética António Xavier (CERMAX). CERMAX has several NMR spectrometers (300, 400, 500 and 800 MHz), including the highest field NMR spectrometer in Portugal. This equipment is part of the National NMR Facility. Contacts: Ricardo Louro (cermax.direction@itqb.unl.pt) available both to ITQB NOVA and outside researchers |
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Mass Spec Facility UniMS UniMS provides state-of-the-art Mass Spectrometry services to the scientific community and Industry, guaranteed by the continuing increase in Mass Spectrometry know-how and infrastructures. Contacts: unims.direction@itqb.unl.pt | unims.direction@ibet.pt available both to ITQB NOVA and outside researchers |
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Microbial Cell Production This facility has the mission to provide technical support to research groups in bacteria/yeast cultivation and associated molecular biology techniques. Contacts: João Carita (carita@itqb.unl.pt) and Teresa Baptista da Silva (teresas@itqb.unl.pt) available to ITQB NOVA researchers |
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Protein Purification & Characterization This facility provides assistance and expertise in protein purification using fast pressure liquid chromatography systems and biochemical characterization of proteins. Contacts: Cristina Timóteo (cristina.timoteo@itqb.unl.pt), Teresa Baptista da Silva (teresas@itqb.unl.pt). available to ITQB NOVA researchers N-terminal Sequencing This facility offers expertise and assistance in protein and peptide N-terminal sequencing (Edman degradation method). Contacts: Paula Chicau (chicau@itqb.unl.pt). available to ITQB NOVA and outside researchers |
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Biophysical Resources This facility has the mission to provide technical support to research groups in several precision instruments for characterization of macromolecules and their interactions. Contacts: Paula Chicau (chicau@itqb.unl.pt) available to ITQB NOVA and outside researchers |
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Small Molecule Analysis The goal of this facility is to provide assistance and technical advice in analytical and semi-preparative high performance chromatography (HPLC). Contacts: Cristina Leitão (mleitao@itqb.unl.pt) available to ITQB NOVA researchers Elemental Analysis Elemental analysis facility provides an accurate determination of carbon, hydrogen, nitrogen and sulfur composition using a Leco TruSpec Micro Elemental Analyzer. Contacts: Conceição Almeida (salmeida@itqb.unl.pt) available to ITQB NOVA and outside researchers |
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Greenhouses & Plant Chambers
The aim of this facility is to provide technical and logistic assistance to plant growth, propagation and protection under controlled environment conditions. Contact: Renato Afonso (renatojta@itqb.unl.pt) available to ITQB NOVA researchers |
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Bacterial Imaging Cluster BIC - facility video The Bacterial Imaging Cluster (BIC) at ITQB NOVA is based on the experience, knowledge and expertise of research groups that are studying different aspects of the bacterial cell biology in order to define new anti-infective strategies against bacterial pathogens. The type of work that is possible at BIC - ITQB NOVA includes, but is not restricted to the following examples: i) the analysis of bacterial cell shape and how it varies using culturing conditions, such as the presence of anti-bacterial agents, and upon genetic lesions; ii) the analysis of bacterial gene expression at the single cell and population levels, through the localization and quantification of the signal from fluorescent reporters or through the direct localization of mRNAs; iii) the study of protein sub-cellular localization during the bacterial cell cycle and differentiation processes; iv) the study of the dynamics of bacterial chromosome replication and segregation. BIC comprises light microscopy instrumentation that is optimized for imaging of fixed or live bacterial cells. It also includes a laser micropoint system coupled to a high-end camera, which allows fluorescence resonance after photobleaching (FRAP) experiments to be implemented. Appropriate filter combinations allow fluorescence resonance energy transfer (or FRET) applications. Image acquisition uses the Metamorph software suite and off-site licenses are available for image analysis and processing. Charges for external uses may be applied. BIC is located on the 5th floor of the ITQB NOVA main building. BIC is ITQB NOVA's node of Plataforma Portuguesa de BioImage PPBI Contacts: Mónica Serrano, Microbial Development Group (serrano@itqb.unl.pt), Pedro Matos Pereira, Intracellular Microbial Infection Biology Group (pmatos@itqb.unl.pt) and Carolina Feliciano, Bacterial Imaging Cluster (cfeliciano@itqb.unl.pt available to ITQB NOVA researchers |
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