Publications
2019
- Santos S.P., Yang Y., Rosa M.T.G., Rodrigues M.A.A., Bouthier De La Tour C., Sommer S., Teixeira M., Carrondo M.A., Cloetens P., Abreu I.A., Romão C.V.* (2019) The interplay between Fe and Mn in Deinococcus radiodurans triggers cellular protection during induced paraquat oxidative stress. Scientific Reports. Accepted
- Martins M.C.#, Romão C.V.#, Folgosa F.#, Borges P.T., Frazão C., Teixeira M.(2019) How superoxide reductases and Flavodiiron Proteins combat oxidative stress in anaerobes FRBM 14, 36-60
https://doi.org/10.1016/j.freeradbiomed.2019.01.051
- Borges P.T, Romão C.V., Saraiva L., Gonçalves V.L., Carrondo M.A., Teixeira M., Frazão C. (2019) Analysis of a new flavodiiron core structural arrangement in Flv1-ΔFlR enzyme from Synechocystis sp. PCC6803, Journal Structural Biology 205, 91-102
https://doi.org/10.1016/j.jsb.2018.11.004
2018
- Romão C. V.*, Matias P.M., Sousa C.M, Pinho F.G., Pinto A.F., Teixeira M., Bandeiras T.M. (2018) Insights into the structures of superoxide reductases from the symbionts Ignicoccus hospitalis and Nanoarchaeum equitans. Biochemistry 57, 5271–5281
https://doi.org/10.1021/acs.biochem.8b00334
- Almadanim M. C., Gonçalves N. M., Rosa M. T. G., Alexandre B. M., Cordeiro A. M, Rodrigues M., Saibo N.J., Soares C. M., Romão C. V., Oliveira M., Abreu I. A. (2018) The rice cold-responsive calcium-dependent protein kinase OsCPK17 is regulated by alternative splicing and post-translational modifications. BBA- Mol Cell Res 1865, 231-246. https://doi.org/10.1016/j.bbamcr.2017.10.010
2017
- Santos S.P., Cuypers M.G., Round A., Finet S., Narayanan T., Mitchell E.P., Romão C.V.* (2017) SAXS structural studies of Dps from Deinococcus radiodurans highlights the conformation of the mobile N-terminal extensions JMB 429, 667-687
http://dx.doi.org/10.1016/j.jmb.2017.01.008
- Lobo S.A.L., Videira M.A.M., Pacheco I., Warren M.J., Teixeira M., Matias P.M., Romão C.V., Saraiva L.M. (2017) Desulfovibrio vulgaris CbiKp cobaltochelatase: evolution of a haem binding protein orchestrated by the incorporation of two histidine residues Environmental Microbiology 19, 106-118
http://dx.doi.org/10.1111/1462-2920.13479
2016-2014
- Martins I., Romão C.V., Goulart J., Cerqueira T., Santos R.S., Bettencourt R. (2016) Activity of antioxidant enzymes in response to atmospheric pressure induced physiological stress in deep-sea hydrothermal vent mussel Bathymodiolus azoricus Marine Environmental Research 114, 65-73
http://dx.doi.org/10.1016/j.marenvres.2016.01.003
- Romão C.V., Vicente J.B., Borges P.T., Frazão, C., Teixeira M. (2016 ) The dual function of flavodiiron proteins: oxygen and/or nitric oxide reductases, J Biol Inorg Chem
http://dx.doi.org/10.1007/s00775-015-1329-4
- Sousa C.M., Carpentier P., Matias P.M., Testa F., Pinho F., Sarti P., Guiffrèe A., Bandeiras T.M., Romão C.V.* (2015) Superoxide reductase from Giardia intestinalis: structural characterization of the first SOR from a eukaryotic organism shows an iron centre highly sensitive to photo-reduction Acta Crystallographic D71, 2236-47 http://dx.doi.org/10.1107/S1399004715015825
- Santos S.P., Mitchell E.P. Franquelim H.G. , Castanho M.A.R.B. , Abreu I.A., Romão C.V. * (2015) Dps from Deinococcus radiodurans: oligomeric forms of Dps1 with distinct cellular functions and Dps2 involved in metal storage FEBS J
http://dx.doi.org/10.1111/febs.13420
- Pinto A.F., Romão C.V., Pinto L.C., Huber H., Saraiva L.M., Todorovic S., Cabelli D., Teixeira M. (2015) Superoxide reduction by a superoxide reductase lacking the highly conserved lysine residue, J Biol Inorg Chem 20, 155-164
http://dx.doi.org/10.1007/s00775-014-1222-6
- Gonçalves V.L., Vicente J.B., Pinto L., Romão C.V., Frazão C., Sarti P., Giuffrè A., Teixeira M. (2014) Flavodiiron oxygen reductase from Entamoeba histolytica: Modulation of substrate preference, J. Biol. Chem 289, 28260-70 http://dx.doi.org/10.1074/jbc.M114.579086
- Borges P. T., Frazão C., Miranda C. S., Carrondo, M.A., Romão C.V.* (2014) Structure of the mono-functional heme catalase DR1998 from Deinococcus radiodurans, FEBS Letters.
http://dx.doi.org/10.1111/febs.12895
- Horch M., Pinto A.F., Utesch T., Mroginskie M.A., Romão C.V., Teixeira M., Hildebrandt P., Zebger I. (2014) Reductive Activation and Structural Rearrangement in Superoxide Reductase: A Combined Infrared Spectroscopic and Computational Study, Phys Chem Chem Phys. http://dx.doi.org/10.1039/c4cp00884g
- Lobo S.A.L., Lawrence A.D., Romão C.V., Warren M.J., Teixeira M., Saraiva L.M., Characterization of Desulfovibrio vulgaris haem b synthase, a radical SAM family member, BBA- Proteins and Proteomics 1877, 1238-1247
http://dx.doi.org/10.1016/j.bbapap.2014.03.016
- Marques A. T., Santos S. P., Rosa M. G., Rodrigues M. A. A., Abreu I. A., Frazão C., Romão C. V.* Expression, purification and crystallization of MnSOD from Arabidopsis thaliana, Acta Cryst. F70, 669-672
http://dx.doi.org/10.1107/S2053230X14007687
- Borges P. T., Miranda C. S., Santos S. P., Carita J. N., Frazao C., Romão C. V.* 2014 Purification, crystallization and phase determination of the DR1998 haem b catalase from Deinococcus radiodurans, Acta Cryst. F70, 659-662
http://dx.doi.org/10.1107/S2053230X1400764X
2011-2012
- Romão C.V, Archer M., Lobo S.A.,Louro R.O., Pereira I.A. C., Saraiva L.M.,Teixeira M. and Matias P.M. (2012) Diversity of Heme Proteins in Sulfate Reducing Bacteria HANDBOOK OF PORPHYRIN SCIENCE - With Applications to Chemistry, Physics, Materials Science, Engineering, Biology and Medicine edited by Kadish K.M., Smith K.M. and Guilard R. 19 (89) pp 139-230, World Scientific Publishing Co. Pte. Ltd., Singapore PDF
- Santos S.P., Bandeiras T.M., Pinto A.F., Teixeira M., Carrondo M.A., Romão C.V. (2012) Thermofluor-based optimization strategy for the stabilization and crystallization of Campylobacter jejuni desulforubrerythrin Protein Expression and Purification 81, 193-200. PDF PUBMED
- Pinho F.G., Pinto, A.F., Pinto L.C., Huber H., Romão C.V., Teixeira M., Matias P.M. and Bandeiras T.M. (2011) Superoxide reductase from Nanoarchaeum equitans: expression, purification, crystallization and preliminary X-ray crystallographic analysis Acta Cryst. F67, 591–595. PDF PUBMED.
- Pinto A.F., Todorovic S., Hildebrandt P., Yamazaki M., Amano F., Igimi S., Romão C.V.* and Teixeira M. Desulforubrerythrin from Campylobacter jejuni, a novel multi-domain protein. J. Biol. Inorg Chem, 16, 501-50. PDF PDF Suppl PUBMED.
- Romão C.V., Ladakis D., Lobo S.A.L., Carrondo M.A., Brindley A.A., Deeryb E., Matias P.M., Pickersgillc R.W., Saraiva L.M., Warren M.J. (2011) Evolution in a family of chelatases facilitated by the introduction of active site asymmetry and protein oligomerization, PNAS, 108, 97-102. PDF PDF Suppl PUBMED
2008-2010
- Pinho, F.G., Romão, C.V., Pinto, A.F., Saraiva, L.M., Huber, H., Matias, P.M., Carrondo, M.A., Teixeira, M. and Bandeiras, T.M., 2010 Cloning, purification, crystallization and X-ray crystallographic analysis of Ignicoccus hospitalis neelaredoxin Acta Cryst. F66, 605-607. PDF PUBMED
- Bandeiras, T.M., Romão C.V., Rodrigues, J.V., Teixeira M., Matias; P.M. (2010) Purification, crystallization and X-ray crystallographic analysis of Archaeoglobus fulgidus neelaredoxin Acta Cryst F66, 316-319. PDF PUBMED
- Toussaint, L., Cuypers M.G., Bertrand L, Hue L., Romão C.V., Saraiva L.M., Teixeira M., Meyer-Klaucke W., Feiters M.C., Crichton R.R. (2009) Comparative Fe and Zn K-edge X-ray absorption spectroscopic study of the ferroxidase centres of human H-chain ferritin and bacterioferritin from Desulfovibrio desulfuricans J.Biol.Inorg.Chem.14, 35-49. PDF PUBMED
- Lobo, S.A.L, Brindley, A.A., Romão, C.V., Leech, H.K., Warren, M.J., Saraiva, L. (2008) Two Distinct Roles for Two Functional Cobaltochelatases (CbiK) in Desulfovibrio vulgaris Hildenborough Biochemistry, 47, 5851–5857. PDF PUBMED
2000-2007
- Cuypers, M, Mitchell, E.P., Romão, C.V., McSweeney, S. (2007), The Crystal Structure of the Dps2 from Deinococcus radiodurans Reveals an Unusual Pore Profile with a Non-specific Metal Binding Site J Mol Biol, 371, 787-799 (COVER). PDF PUBMED
- Romão, C.V., Mitchell, E.P., McSweeney, S. (2006) The crystal structure of Deinococcus radiodurans Dps protein (DR2263) reveals the presence of a novel metal centre in the N-terminus, J.Biol.Inorg.Chem., 11, 891-902. PDF PUBMED
- McVey, C.E., Amblar, M. , Barbas, A., Cairrão, F., Coelho, R., Romão, C. Arraiano, C.M., Carrondo, M.A. and Frazão, C. (2006) Expression, purification, crystallization and preliminary diffraction data characterization of Escherichia coli ribonuclease II (Rnase II) Acta Cryst F62, 684-687. PDF PUBMED
- Almeida, C.C., Romão, C.V., Lindley, P.F., Teixeira, M. and Saraiva, L.M. (2006) The role of the Hybrid Cluster Protein in Oxidative Stress Defense J Biol Chem 281 32445-32450. PDF PUBMED
- Aragão D., Macedo S., Mitchell E.P., Romão C.V., Liu M.Y., Frazao C., Saraiva L.M., Xavier A.X., LeGall J., van Dongen W.M.A.M. , Hagen W.R., Teixeira M., Carrondo M.A. and Lindley P. (2003) Reduced hybrid cluster proteins (HCP) from Desulfovibrio desulfuricans ATCC 27774 and Desulfovibrio vulgaris (Hildenborough): X-ray structures at high resolution using synchrotron radiation J Biol Inorg Chem 8, 540–548. PDF PUBMED
- Macedo, S., Romão, C.V., Mitchell, E.P., Matias, P.M., Liu, M.-Y., Xavier, A.V., LeGall, J., Teixeira, M., Lindley, P. and Carrondo, M.A. (2003) The nature of the di-iron site in the bacterioferritin from Desulfovibrio desulfuricans, Nat. Strut. Biol.,10, 285-290. PDF PUBMED
- Macedo, S., Mitchell, E.P., Romão, C.V., Cooper, S.J., Coelho, R., Liu, M.-Y., Xavier, A.V., LeGall, J., Bailey, S., Garner, D. Hagen, W.R., Teixeira, M., Carrondo, M.A. and Lindley, P. (2002) Hybrid cluster proteins (HCPs) from Desulfovibrio desulfuricans ATCC 27774 and Desulfovibrio vulgaris (Hildenborough): X-ray structures at 1.25 Å resolution using synchrotron radiation, J.Biol.Inorg. Chem., 7, 514-525. PDF PUBMED
- da Costa, P.N., Romão, C.V., LeGall, J., Xavier, A.V., Melo, E., Teixeira, M. and Saraiva, L.M. (2001) The genetic organization of Desulfovibrio desulfuricans ATCC 27774 bacterioferritin and rubredoxin-2 genes: involvement of rubredoxin in iron metabolism, Mol. Microbiol, 41, 217-227. PDF PUBMED
- Romão, C.V., Louro, R., Timkovich, R., Lübben, M., Liu, M.-Y., LeGall, J., Xavier, A.V., Teixeira, M. (2000) Iron-Coproporphyrin III a bacterioferritin from the strict anaerobe Desulfovibrio desulfuricans ATCC 27774, FEBS Lett, 480, 213-216. PDF PUBMED
- Romão, C.V., Regalla, M., Liu, M.-Y., LeGall, J., Xavier, A.V., Teixeira, M. (2000) A bacterioferritin from the strict anaerobe Desulfovibrio desulfuricans ATCC 27774, Biochemistry, 39, 6841-6849. PDF PUBMED
1997-1999
- Romão, C.V.; Liu, M.Y.; LeGall, J.; Gomes, C.M.; Braga, V.; Pacheco, I.; Xavier, A.V.; Teixeira, M. (1999) The superoxide dismutase activity of desulfoferrodoxin from Desulfovibrio desulfuricans ATCC 27774, Eur. J. Biochem. 261, 438-443. PDF PUBMED
- Pereira, I.C.; Romão, C.V.; Xavier, A.V.; LeGall, J.; Teixeira, M. (1998) Electron transfer between hydrogenases and mono- and multiheme cytochromes in Desulfovibrio ssp, J.Biol.Inorg. Chem. 8, 494-498. PDF
- Romão, C.V.; Pereira, I.C.; Xavier, A.V.; LeGall, J.; Teixeira, M. (1997) Characterization of the [NiFe] Hydrogenase from the sulfate reducer Desulfovibrio vulgaris Hildenborough, Biochem. Biophys. Res. Comm. 240, 75-79. PDF PUBMED
