José Parreira Salvado

PhD student - "Plants for Life" program

Name:  José Ricardo Torrão Dias Parreira Salvado

Citizenship:  Portuguese
 

 

ITQB NOVA – Plant Cell Biotechnology Laboratory

Instituto de Tecnologia Química e Biológica António Xavier
Universidade Nova de Lisboa
Av. da República, 2780-157 Oeiras – PORTUGAL
Tel: (351) 214 469 461   Fax: (351) 214 411 277

 

Keywords

 

Common bean, Phaseolus vulgaris, seed development, transcriptomics, proteomics


 Academic qualifications

2008 - Masters in Cellular Biology and Biotechnology, Faculty of Sciences, University of Lisbon, Portugal.

2007 - Degree in Cellular Biology and Biotechnology, Faculty of Sciences, University of Lisbon.

 

Professional and research experience

Mar. 2015 – present: PhD student, Plant Cell Biotechnology Laboratory, ITQB, Universidade Nova de Lisboa, Portugal. Project: Unravelling seed development in Phaseolus vulgaris L.

Apr. 2014 - Feb. 2015: Researcher in the project “Lactation and milk production in goat (Capra hircus): identifying molecular markers underlying adaptation to seasonal weight loss.” PTDC/CVT/116499/2010. IBET - Instituto de Biologia Experimental Tecnológica and Faculdade de Medicina Veterinária da Universidade de Lisboa, Portugal.

Oct. 2012 - Mar. 2014: Researcher in the project “Deciphering grain filling mechanisms in Phaseolus vulgaris L. under water deficit” PTDC/AGR-GPL/110244/2009 - IICT, Instituto de Investigação Científica Tropical, and ITQB, Instituto de Tecnologia Química e Biológica, Oeiras, Portugal.

Jan. 2009 - Dec. 2010: Researcher in the project "Estudio de resistencia antibiótica, virulencia y epidemicidad en el microorganismo patógeno nosocomial Acinetobacter baumannii, mediante análisis proteómico" Instituto de Investigaciones Biomédicas de A Coruña (I.N.I.B.I.C.) Laboratorio de Microbiología y Patologías Infecciosas, A Coruña, Spain.

 

Attended courses

2016 - “Analysis and Integration of Transcriptome and Proteome Data”. EMBL Course. Feb. 14th-19th. EMBL, Heidelberg, Germany.

2015 - “Analysis of Small Non-coding RNAs: per aspera ad astra”. EMBO Practical Course. Jul. 25th-31st - CEITEC Campus, Brno, Czech Republic.

2014 - “Transcriptome Assembly, Automatic Annotation and Data Mining”, The Gulbenkian Training Programme in Bioinformatics - Oct. 6th-9th - Instituto Gulbenkian de Ciência, Oeiras, Portugal.

2012 - “Biostatistical Foundations in Bioinformatics” - Dec. 4th-7th - Instituto Gulbenkian de Ciência.

2012 - “Hunting for Promoters in NGS Data”, The Gulbenkian Training Programme in Bioinformatics - Oct. 22nd-25th - Instituto Gulbenkian de Ciência.

 

Technical expertise

Member of R&D projects

- “egoSELF: An Integrative Biology approach to study the molecular basis of inbreeding depression in Eucalyptus globulus”. PTDC/AGR-FOR/0931/2014. Funded by Fundação para a Ciência e a Tecnologia, Portugal.

- “Unravelling grain filling mechanism in Phaseolus vulgaris L. under water stress: a proteomics approach”. EU-FP7-PRIME-XS/288 - European FP7 Proteomics Infrastructure.

- “Lactation and milk production in goat (Capra hircus): identifying molecular markers underlying adaptation to seasonal weight loss.” PTDC/CVT/116499/2010. ITQB, IBET and Faculdade de Medicina Veterinária. Funded by Fundação para a Ciência e a Tecnologia, Portugal.

- “Deciphering grain filling mechanisms in Phaseolus vulgaris L. under water deficit”. PTDC/AGR-GPL/110244/2009. IIICT and ITQB. Funded by Fundação para a Ciência e a Tecnologia, Portugal.

- "Estudio de resistencia antibiótica, virulencia y epidemicidad en el microorganismo patógeno nosocomial Acinetobacter baumannii, mediante análisis proteómico”. CHUAC and INIBIC, A Coruña, Spain.

Publications

Articles

Cugno G., Parreira J.R., Ferlizza E., Hernández-Castellano L.E., Carneiro M., Renaut J., Castro N., Arguello A., Capote J., Campos A.M., Almeida A.M. (2016) The goat (Capra hircus) mammary gland mitochondrial proteome: a study on the effect of weight loss using Blue-Native PAGE and two-dimensional gel electrophoresis. PLoS One 11(3):e0151599.   [Full text links - Open Access]

Parreira J.R., Bouraada J., Fitzpatrick M.A., Silvestre S., Bernardes da Silva A.B., Marques da Silva J., Almeida A.M., Fevereiro P., Altelaar A.F.M., Araújo S.S. (2016) Differential proteomics reveals the hallmarks of seed development in common bean (Phaseolus vulgaris L.). Journal of Proteomics 143: 188-198.   [Abstract and full text links]

Almeida A.M., Parreira J.R., Santos R., Duque A.S., Francisco R., Tomé D.F.A., Ricardo C.P., Coelho A.V., Fevereiro P. (2012) A proteomics study of the induction of somatic embryogenesis in Medicago truncatula using 2DE and MALDI-TOF/TOF. Physiologia Plantarum 146: 236–249.   [Abstract and full text links]

Cabral M.P., Soares N.C., Aranda J., Parreira J.R., Rumbo C., Poza M., Valle J., Calamia V., Lasa Í., Bou G. (2011) Proteomic and functional analyses reveal a unique lifestyle for Acinetobacter baumannii biofilms and a key role for histidine metabolism. Journal of Proteome Research 10: 3399-3417.   [Abstract and full text links]

Aranda J., Poza M., Pardo B.G., Rumbo S., Rumbo C., Parreira J.R., Rodríguez-Velo P., Bou G. (2010) A rapid and simple method for constructing stable mutants of Acinetobacter baumannii. BMC Microbiology 10:279.   [Full text links - Open Access]

Soares N.C., Cabral M.P., Parreira J.R., Gayoso C., Barba M.J., Bou G. (2009) 2-DE analysis indicates that Acinetobacter baumannii displays a robust and versatile metabolism. Proteome Science 7:37.   [Full text links - Open Access]

Book chapters

Parreira J.R., Araújo S.S. (2018) Studying the animal transcriptome: state of the art and challenges in the context of animal and veterinary sciences. In: Almeida A.M., Eckersall D., Miller I., eds. Proteomics in Domestic Animals: from Farm to Systems Biology (in press). Springer.   [Book information]

Parreira J.R., Branco D., Almeida A.M., Czubacka A., Agacka-Moldoch M., Paiva J.A.P., Tavares-Cadete F., Araújo S.S. (2016) Systems biology approaches to improve drought stress in plants: state of the art and future challenges. In: Hossain M.A., Wani S.H., Bhattachajee S., Burritt D., Tran L.S.P., eds. Drought stress tolerance in plants. Volume 2 - Molecular and Genetic Perspectives. pp. 433-471. Springer, Cham.   [Abstract and full text links]

Thesis

Parreira Salvado, J.R. (2008) “A study of the differentially expressed proteins during the induction of somatic embryogenesis in Medicago truncatula – a proteomic approach”. Master thesis, Faculty of Sciences, University of Lisbon, Portugal. Supervisor: Dr. Pedro Fevereiro.

Conference proceedings

Almeida A.M., Parreira J., Francisco R., Santos R., Duque S., Tomé D., Ricardo C.P., Coelho A., Fevereiro P.S. (2010) Proteomics of somatic embryogenesis in the model legume barrel medic (Medicago truncatula). 4th EuPA Scientific Meeting – A Proteomics Odyssey towards next decades: 238-240. Edited by G. Marko-Varga and T. Simões, EUPA – European Proteomics Association. Estoril, Portugal. ISBN: 978-963-86156-5-7.


 Posters

1) “Protein metabolism during seed development in Phaseolus vulgaris L.” Diving into integrative cell phenotyping though “omics”. COST Action FA1306. 1-2 Feb. 2016, Versailles, France.

2) “Using proteomics to understand seed development in Phaseolus vulgaris L.: developing resources to modulate seed quality traits”. 110° Congresso della Società Botanica Italiana. 14-18 Sep. 2015, Pavia, Italy.

3) “NGS-based gene expression profile study in the goat mammary gland: the effect of undernutrition in two breeds with different levels of adaptation to nutritional stress”. ADSA-ASAS Joint Annual Meeting. 12-16 Jul. 2015, Orlando, Florida, USA.

4) “Unraveling seed development mechanism in Phaseolus vulgaris L.: a proteomic approach” The quest for tolerant varieties: phenotyping at plant and cellular level. COST Action FA1306. 22-24 Jun. 2015, Gatersleben, Germany.

 5) “Unraveling seed development mechanism in Phaseolus vulgaris L. under water deficit: a transcriptomic approach” The quest for tolerant and stable varieties I: the role of “omics” - COST Action FA1306. 26-27 Feb. 2015, Carcavelos, Portugal.

6) “Developing an RNA extraction method for Next Generation Sequencing (NGS)-based transcriptomics studies in the goat mammary gland”. First Dairy Care Conference. 22-23 Aug. 2014, Copenhagen, Denmark.

7) “Unraveling grain filling mechanisms in P. vulgaris L. under water deficit: a transcriptomics and proteomic approach”. 6th International Food Legumes Research Conference and 7th International Conference on Legume Genetics and Genomics. 6-11 Jul. 2014, Saskatoon, SK, Canada.

8) “An optimized RNA Extraction protocol for leaves, pods and seeds from Phaseolus vulgaris L.” XIII Congresso Luso-Espanhol de Fisiologia Vegetal - 24-28 July 2013, Lisbon, Portugal.

9) “An optimized RNA Extraction protocol for leaves, pods and seeds from Phaseolus vulgaris L.” 1st Legume Society Conference. 8-11 May 2013. Novi Sad, Serbia.

10) “Proteins expressed during the induction of somatic embryogenesis in the model legume Medicago truncatula”, poster presented at the COST FA0603 WG1 Meeting: Technical aspects inherent to Plant Proteomics, May 2009,  Viterbo, Italy.

Meetings Organization, Science Communication

- “VI Encontro - Biotecnologia e Agricultura: O Futuro é Agora”. 13 Jul. 2017, Campus da Foz, Universidade Católica Portuguesa, Porto, Portugal.

- “Workshop on legume transformation: old and new genetic engineering techniques to cope with environmental challenges”. 26-30 June 2017, ITQB NOVA, Oeiras, Portugal.

- “V Encontro - Biotecnologia e Agricultura: O Futuro é Agora”. 16 Oct. 2015, Coimbra, Portugal.

- “Drawing the fascination for plants in sketchbooks”. Plant Fascination Week. 18-22 May 2015, ITQB, Oeiras.

- “3rd Forest Genomics Meeting: Regulation of genome expression dynamics in forest tree”. 3 Dec. 2014, Oeiras.

- “IV Encontro - Biotecnologia e Agricultura: O Futuro é Agora”. 11 Jun. 2014, Santarém, Portugal.

- “Integrating legume biology for sustainable agriculture”. 12-16 Nov. 2007, Lisbon, Portugal.

Honours, Prizes and Scholarships

- PhD scholarship on the “Plants for Life” PhD Program 2015 (PD/BD/113474/2015). ITQB, Oeiras, Portugal.

- EMBL Corporate Partnership Travel Grant to attend: Analysis and Integration of Transcriptome and Proteome Data. EMBL Course. 14-19 Feb. 2016. EMBL, Heidelberg, Germany.

- Travel grant to attend: Diving into integrative cell phenotyping though “omics” (COST Action FA1306): 1-2 Feb. 2016, Versailles France.

- Travel grant to attend: 110° Congresso della Società Botanica Italiana. 14-18 Sep. 2015, Pavia, Italy.

- EMBL Corporate Partnership Registration Fee waiver to attend: Analysis of Small Non-coding RNAs: per aspera ad astra. EMBO Practical Course. 25-31 Jul. 2015, CEITEC Campus, Brno, Czech Republic.

- Travel grant to attend: The quest for tolerant varieties: phenotyping at plant and cellular level (COST Action FA1306): 22-24 Jun. 2015, Gatersleben, Germany.

- Travel grant to attend: First Dairy Care Conference - COST Action FA1308. 22-23 Aug. 2014, Copenhagen, Denmark.

- Research scholarship on the FCT project PTDC/CVT/116499/201.

- Research scholarship on the FCT project PTDC/AGR-GPL/110244/2009.

- Contract as a biology/scientific researcher in Fundación Complexo Hospitalario Universitario A Coruña, Spain.

- Member of the Legume Society (http://ils.nsseme.com/).

- Member of the Portuguese Society of Plant Physiology.

- Member of the COST Action FA1306 - “The quest for tolerant varieties - Phenotyping at plant and cellular level”.

- Member of the COST Action FA1308 - “DairyCare”.

- 3rd Place on the "Paixão pelo desenho, amor pelo Ambiente", FNAC/Caran d’Ache.

 

Plant Cell Biotechnology Laboratory

 

Last updated on 28 December 2017