Program
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| DAY 1: Thursday 22 September 2011 | |
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| Location: Instituto Gulbenkian de Ciência - IGC | |
| Xavier Salvatella and Santi Esteban-Martin Institute For Research in Biomedicine (Barcelona, Spain) | |
| 9h00 – 11h00 | Introduction to protein dynamics and molecular dynamics simulations |
| 11h00 – 11h30 | Coffee break |
| 11h30-12h30 | Effect of molecular dynamics on NMR parameters - NMR as a tool to study molecular dynamics |
| 12h30 – 14h00 | Lunch |
| 14h00-16h00 | Tutorial on Molecular Dynamics |
| 16h00-16h30 | Coffee Break |
| 16h30-18h00 | Tutorial on back-calculation of NMR parameters from trajectories |
| DAY 2: Friday 23 September 2011 | |
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| Location: Instituto Gulbenkian de Ciência - IGC | |
| Antonio Pineda-Lucena and Leonor Puchades Prince Felipe Research Centre (Valencia, Spain) | |
| 9h00 – 11h00 | Rational Drug Design |
| 11h00 – 11h30 | Coffee break |
| 11h30-12h30 | Utilities: Protein databases, visualization, computational tools |
| 12h30 – 14h00 | Lunch |
| 14h00-16h00 | Chemoinformatics/Protein-ligand docking with AutoDock |
| 16h00-16h30 | Coffee Break |
| 16h30-18h00 | AutoDock tutorial |
| DAY 3: Saturday 24 September 2011 | |
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| Location: Instituto Gulbenkian de Ciência - IGC | |
| Cameron Mackereth and Samir Amrane Institut Européen de Chimie Biologique (Bordeaux, France) | |
| 9h00 – 11h00 | Protein-protein interactions Introduction to the various classes of protein-protein association |
| 11h00 – 11h30 | Coffee break |
| 11h30-12h30 | Short-range protein-protein contacts Highlight current methods to determine direct contacts between residues. |
| 12h30 – 14h00 | Lunch |
| 14h00-16h00 | Long-range distance information Use of paramagnetic relaxation enhancement to characterize long-range distances and transient complexes. Incorporating complementary methods such as small-angle scattering (SAXS/SANS). Practical. |
| 16h00-16h30 | Coffee Break |
| 16h30-18h00 | Domain-domain orientation Residual dipolar coupling to look at the orientation of protein domains in both stable and dynamic complexes. Practical. |
| DAY 4: Monday 26 September 2011 | |
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| Location: Instituto Gulbenkian de Ciência - IGC | |
| Tim Stevens and Wayne Boucher | |
| 9h00 – 11h00 | Loading spectra Spectrum window navigation |
| 11h00 – 11h30 | Coffee break |
| 11h30-12h30 | Peak picking and assignment |
| 12h30 – 14h00 | Lunch |
| 14h00-16h00 | Assigning resonances & spin systems |
| 16h00-16h30 | Coffee Break |
| 16h30-18h00 | Sequential Assignment |
| DAY 5: Tuesday 27 September 2011 | |
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| Location: Instituto Gulbenkian de Ciência - IGC | |
| Tim Stevens, Wayne Boucher and Nina Luckgei | |
| 9h00 – 11h00 | Solid state spectra & experiment types |
| 11h00 – 11h30 | Coffee break |
| 11H30-12H30 | Using and defining isotope labelling schemes |
| 12h30 – 14h00 | Lunch |
| 14h00-16h00 | Assigning a mixed 13C/12C glycerol labelled sample |
| 16h00-16h30 | Coffee Break |
| 16h30-18h00 | Assigning double quantum spectra |
| DAY 6: Wednesday 28 September 2011 | |
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| Location: Instituto de Tecnologia Química e Biológica - ITQB | |
| Solid State Software Development Group Meeting | |
| 9h00-9h30 | Protein ssNMR and beyond Abhishek Cukkemane Bijvoet Center for Biomolecular Research (Utrecht, The Netherlands) |
| 9h30-10h00 | 3D assignments strategies for solid proteins Anja Bockmann Institut de Biologie et Chimie des Protéines (Lyon, France) |
| 10h00-10h30 | 13C spin dilution for resonance assignment and detection of distance restraints Adam Lange Max Planck Institute for Biophysical Chemistry (Göttingen, Germany) |
| 10h30-10h50 | Sequential backbone assignment, based on 1H-detection at ultra-fast MAS Guido Pintacuda CNRS / Ecole Normale Supérieure de Lyon |
| 10h50-11h20 | Coffee break |
| 11h20-11h50 | Developments for v3 and solid state in CCPN software Tim Stevens University of Cambridge (Cambridge, United Kingdom) |
| 11h50-12h30 | Discussion |
| 3rd EU/CCPN Conference | |
| NMR applications in Solution | |
| 12h00 – 12h30 | REGISTRATION |
| 12h30-13h45 | Lunch |
| 13h45 | Meeting starts |
13h50-14h00 | Welcome and Introduction Applications in Solution Annalisa Pastore National Institute for Medical Research -NIMR (London, United Kingdom) |
| 14h00 – 14h30 | To solve the structure of a protein DNA complex involving the DNA binding domain of Ler, a member of the H-NS family of proteins Tiago Cordeiro Institut de Recerca Biomèdica (Barcelona, Spain) |
| 14h30 – 15h00 | Structure and interactions of HMGB1 Katherine Stott University of Cambridge (Cambridge, United Kingdom) |
| 15h00 – 15h30 | Structural studies of the Nucleosome by NMR Hugo van Ingen University of Utrecht (Utrecht, Netherlands) |
| 15h30 – 16h00 | Tea |
| 16h00 – 16h30 | Structural studies of the regulatory domain of PLCbamma1 Diego Esposito National Institute for Medical Research -NIMR (London, United Kingdom) |
| 16h30 – 17h15 | New and different CcpNmr software Rasmus Fogh, Tim Stevens University of Cambridge (Cambridge, United Kingdom) |
| 17h15 – 18h00 | Discussion |
| 18h00-19h00 | Poster Session |
| 19h45 | Dinner |
| DAY 7: Thursday 29 September 2011 | |
|---|---|
| Location: Instituto de Tecnologia Química e Biológica - ITQB | |
| NMR applications in Solids | |
| 8h00 | Breakfast (at the Hotel) |
| 8h50 | Meeting starts |
8h50 – 9h00 | Applications in Solids Hartmut Oschkinat Leibniz-Institut für Molekulare Pharmakologie (Berlin, Germany) |
| 9h00 – 9h30 | Structure and Dynamis of Prions by Solid-State NMR Beat Meier Eidgenössische Technische Hochschule (Zürich, Germany) |
| 9h30 – 10h00 | Solid-State NMR studies of Alzheimer's disease beta-amyloid aggregates Bernd Reif Technische Universität München (München, Germany) |
| 10h00 – 10h30 | Coffee break |
| 10h30 – 11h00 | Solid-State NMR studies of the Sup35 prion Nina Luckgei Institut de Biologie et Chimie des Protéines (Lyon, France) |
| 11h00 – 11h30 | De novo Structure of Yad A Membrane Anchor Domain by Solid-State NMR Shakeel Ahmad Shahid Leibniz-Institut für Molekulare Pharmakologie (Berlin, Germany) |
| 11h30 – 12h15 | Discussion |
| 12h15 – 13h00 | Lunch |
| 13h00 – 14h30 | Poster Session |
| 15h00 – 19h | Depart for coastal walk |
| 20h | Conference dinner Poster Prize Presentation |
| DAY 8: Friday 30 September 2011 | |
|---|---|
| Location: Instituto de Tecnologia Química e Biológica - ITQB | |
| Protein Expression | |
| 8h00 | Breakfast (at the Hotel) |
| 8h50 | Meeting starts |
8h50 – 9h00 | Protein Expression Imré Berger European Molecular Biology Laboratory (Heidelberg, Germany) |
| 9h00 – 9h30 | Multi-expression technologies for complex production in prokaryotic and eukariotic hosts Imré Berger European Molecular Biology Laboratory (Heidelberg, Germany) |
| 9h30 – 10h00 | ESPRIT and CoESPRIT: Soluble fragment screening for structural biology Darren Hart European Molecular Biology Laboratory (Heidelberg, Germany) |
| 10h00-10h30 | Strategies for cell-free expression and NMR structural characterisation of alpha-helical membrane proteins Sina Reckel Goethe University (Frankfurt, Germany) |
| 10h30 – 11h00 | Coffee break |
| 11h00 – 11h30 | Preparation of membrane protein samples for solution NMR studies Sebastian Hiller Biozentrum, University of Basel (Basel, Switzerland) |
| 11h30 – 12h15 | Discussion |
| 12h15 – 12h35 | Discussion for overall meeting |
| 12h35 - 12h45 | Closing remarks Annalisa Pastore |
| 12h45 – 13h30 | Lunch |
| 13h35 | Conference ends |
